A Pilot Study of the Tuning in to Kids Parenting Program in Iran for Reducing Preschool Children�s Anxiety

Objectives: Tuning in to Kids (TIK) is a group parenting program that targets parent emotion socialization (emotional awareness and regulation, meta-emotion and emotion coaching skills) to improve children�s functioning. The aim of this pilot study was to investigate the effectiveness of this program when used with parents of anxious preschool children. Methods: The study used a randomized controlled design. Fifty-six mothers of children who scored one standard deviation above the mean on the parent report of the Preschool Anxiety Scale (PAS) were selected from 358 families who expressed interest in participating in a parenting program. Selected participants were randomly assigned to intervention (n = 30) or control (n = 26) conditions. Participants in the intervention condition attended the 6 session TIK program followed by two booster sessions at monthly intervals thereafter. Post-intervention and 6- months later the PAS was re-administered to participants in both conditions. Results: Mixed Repeated Measure ANOVA analysis showed a significant difference between participants in the two conditions on parent-reported anxiety at post-test and 6-month follow-up. Clinical significance analyses showed 69 of the intervention group in comparison to 18 of the control group had parent-reported change into the normal range for anxiety scores. These changes remained stable at 6-month follow-up (60 compared to 23). Conclusion: The study suggests that the TIK program shows preliminary effectiveness when used in Iran with preschool children with anxiety. © 2019, The Author(s)

Quantitative Analysis of Histone Modifications: Formaldehyde Is a Source of Pathological N6-Formyllysine That Is Refractory to Histone Deacetylases

Aberrant protein modifications play an important role in the pathophysiology of many human diseases, in terms of both dysfunction of physiological modifications and the formation of pathological modifications by reaction of proteins with endogenous electrophiles. Recent studies have identified a chemical homolog of lysine acetylation, N[superscript 6]-formyllysine, as an abundant modification of histone and chromatin proteins, one possible source of which is the reaction of lysine with 3′-formylphosphate residues from DNA oxidation. Using a new liquid chromatography-coupled to tandem mass spectrometry method to quantify all N[superscript 6]-methyl-, -acetyl- and -formyl-lysine modifications, we now report that endogenous formaldehyde is a major source of N[superscript 6]-formyllysine and that this adduct is widespread among cellular proteins in all compartments. N[superscript 6]-formyllysine was evenly distributed among different classes of histone proteins from human TK6 cells at 1–4 modifications per 10[superscript 4] lysines, which contrasted strongly with lysine acetylation and mono-, di-, and tri-methylation levels of 1.5-380, 5-870, 0-1400, and 0-390 per 10[superscript 4] lysines, respectively. While isotope labeling studies revealed that lysine demethylation is not a source of N[superscript 6]-formyllysine in histones, formaldehyde exposure was observed to cause a dose-dependent increase in N[superscript 6]-formyllysine, with use of [[superscript 13]C,[superscript 2]H[subscript 2]]-formaldehyde revealing unchanged levels of adducts derived from endogenous sources. Inhibitors of class I and class II histone deacetylases did not affect the levels of N[superscript 6]-formyllysine in TK6 cells, and the class III histone deacetylase, SIRT1, had minimal activity (<10%) with a peptide substrate containing the formyl adduct. These data suggest that N[superscript 6]-formyllysine is refractory to removal by histone deacetylases, which supports the idea that this abundant protein modification could interfere with normal regulation of gene expression if it arises at conserved sites of physiological protein secondary modification

Abstract Number ‐ 271: Comorbidities: Severe Stroke in Thrombolysis for Ischemic Stroke with Elevated Diastolic Blood Pressure

Introduction Patients are at risk for different outcomes following stroke based on their diastolic blood pressure on admission. In this study, we determine differences in risk factors between patients with DBP  = 80 mmHg and then determine which of these risk factors are associated with worsening neurologic outcomes after rtPA administration. Methods A retrospective analysis was performed by looking at demographics, history, and clinical factors that are known risk factors for acute ischemic stroke. Data were obtained from a stroke registry that includes patient data from between 2010 and 2016. Univariate analysis was performed based on presenting diastolic blood pressure group. Patients who did not receive rtPA were excluded from further study and then patients were further divided by NIHSS > = 7 or > 7. Logistic regression was used to further evaluate variables. Odds Ratios with 95% Confidence Intervals (C.I.) were calculated and used to predict worsening neurologic outcomes for stroke patients based on their blood‐pressure group. Results In the population of stroke patients with diastolic blood pressure  = 80 mmHg, increasing age (OR = 1.026, 95% C.I. = 1.012 – 1.04. P‐value =  = 80 mmHg with acute ischemic stroke and these groups also had different risk factors for severe stroke after rtPA administration. In both groups, increasing age was associated with worsening neurologic outcomes, while direct admission was associated with improving outcomes. In the DBP  = 80 mmHg group, a history of alcohol or drug use and increased heart rate were associated with worsening neurologic outcomes