Pengaruh Pemberian Susu Kuda Fermentasi Terhadap Antibodi Imunoglobulin a (Iga) Mencit Setelah Vaksinasi Hepatitis A

IgA-deficient in individuals have developed mucosal infections, atopy, autoimmune diseasesand rheumatic. Under the circumstance, the development of an immunomodulator that stimulatesimmune system, especially IgA, is truly desired. The research is objected on finding out the effect offermented horse milk treatment on IgA antibody in Hepatitis A immunized mice. IgA concentrationin serum and lymphocytes was evaluated using indirect ELISA method. Fermented horse milkgroup was compared with water group to calculate the percentage of IgA concentration increase.Univariate analysis was used to analyze Optical Density (OD) product from ELISA. The resultshowed that fermented horse milk increased 46.20 % IgA concentration in serum and 95.47 % inspleen lymphocytes. Univariate analysis showed that fermented horse milk increased significantlyIgA spleen lymphocytes but not IgA serum

Pemanfaatan Kulit Telur Ayam dan Abu Layang Batubara sebagai Katalis Heterogen untuk Reaksi Transesterifikasi Minyak Nyamplung (Calophyllum Inophyllum Linn)

Padatan CaO telah berhasil dipreparasi dengan kalsinasi kulit telur ayam pada suhu 1000 ºC. Hasil karaterisasi dengan XRD dan SEM menunjukkan bahwa CaO yang dihasilkan memiliki pola difraktogram serta morfologi yang sama dengan CaO standar. Padatan CaO hasil preparasi tersebut ditanamkan pada permukaan abu layang dengan metode impregnasi, sehingga diperoleh katalis CaO/abu layang dan CaO/abu layang leaching. Hasil karakterisasi dengan SEM dan FTIR menunjukkan bahwa CaO menempel pada permukaan abu layang, serta membentuk ikatan Si-O-Ca dengan silika dari abu layang. Hasil uji katalis pada reaksi transesterifikasi minyak nyamplung dengan metanol menunjukkan bahwa kadar FAME paling besar dicapai oleh katalis 75CFAL, diikuti oleh katalis 75CFA, 50CFAL, CaO, dan 50CFA. Katalis CaO dengan pendukung abu layang menunjukkan aktivitas lebih tinggi dari pada katalis Ca

Uji Aktivitas Imunostimulator Fraksi Etil Asetat Ekstrak Etanol Kelopak Bunga Rosella (Hibiscus Sabdariffa L.) Terhadap Proliferasi Sel Limfosit Mencit Galur Swiss Secara in Vitro Beserta Identifikasi Kandungan Senyawa Kimianya

Immunostimulatory agent has an important role in the treatment of infectious disease caused by pathogen. Roselle calyx (Hibiscus sabdariffa L.) used by community as a health drink to enhance body\u27s immune system. The aims of this research was to know immunostimulatory activity of ethyl acetate fraction of ethanolic extract of roselle calyx on lymphocyte cell proliferation Swiss strain mice and to identified the chemical compounds of the fraction. Roselle calyx was extracted by maceration method using ethanol 96% as a solvent. The ethanolic extract was partitioned gradually by n-hexan and ethyl acetate. Series of ethyl acetate fraction were made to concentration 10, 20, 50, 100, 200, 400 μg/mL and positive control PHA 10 μg/mL for tested to lymphocyte cell culture. Immunostimulatory activity test was done according to MTT Assay method. Lymphocyte proliferation activity was analyzed statistically to the value of Optical Density (OD) result of the Enzyme Linked Immunosorbent Assay (ELISA) reader using Kruskal Wallis Test continued by Mann Whitney Test (p<0,05). Identification of the contents phenolic compounds and flavonoids performed by Thin Layer Chromatography (TLC). The result of the research showed that ethyl acetate fraction had lymphocyte cell proliferation activity at the concentration of 50, 100, 200, and 400 μg/mL. The ethyl acetate fraction of roselle calyx contained phenolic compounds and flavonoids

Uji Aktivitas Imunostimulator Fraksi Etil Asetat Ekstrak Etanol Kelopak Bunga Rosella (Hibiscus Sabdariffa L.) Terhadap Proliferasi Sel Limfosit Mencit Galur Swiss Secara in Vitro Beserta Identifikasi Kandungan Senyawa Kimianya

Immunostimulatory agent has an important role in the treatment of infectious disease caused by pathogen. Roselle calyx (Hibiscus sabdariffa L.) used by community as a health drink to enhance body's immune system. The aims of this research was to know immunostimulatory activity of ethyl acetate fraction of ethanolic extract of roselle calyx on lymphocyte cell proliferation Swiss strain mice and to identified the chemical compounds of the fraction. Roselle calyx was extracted by maceration method using ethanol 96% as a solvent. The ethanolic extract was partitioned gradually by n-hexan and ethyl acetate. Series of ethyl acetate fraction were made to concentration 10, 20, 50, 100, 200, 400 μg/mL and positive control PHA 10 μg/mL for tested to lymphocyte cell culture. Immunostimulatory activity test was done according to MTT Assay method. Lymphocyte proliferation activity was analyzed statistically to the value of Optical Density (OD) result of the Enzyme Linked Immunosorbent Assay (ELISA) reader using Kruskal Wallis Test continued by Mann Whitney Test (p<0,05). Identification of the contents phenolic compounds and flavonoids performed by Thin Layer Chromatography (TLC). The result of the research showed that ethyl acetate fraction had lymphocyte cell proliferation activity at the concentration of 50, 100, 200, and 400 μg/mL. The ethyl acetate fraction of roselle calyx contained phenolic compounds and flavonoids

Pengaruh Infusa Wortel (Daucus Carota L.) Terhadap Histopatologi Ginjal Tikus Jantan Yang Diinduksi Uranium

Ginjal merupakan organ vital yang berperan sangat penting dalam mempertahankan kestabilan lingkungan dalam tubuh dengan fungsi utama yaitu filtrasi oleh glomerulus, reabsorbsi dan sekresi tubulus. Kerusakan sel ginjal sampai kematian sel akan menyebabkan fungsi ginjal terganggu. Efek paparan senyawa radioaktif salah satunya dapat menyebabkan terjadinya gangguan sel-sel ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh infusa wortel (Daucus carota L.) dalam mencegah gangguan sel ginjal akibat paparan uranium. Uji proteksi dilakukan dengan membagi 30 tikus jantan dewasa menjadi enam kelompok masing-masing kelompok terdiri dari 5 ekor. Kelompok I tanpa diberi perlakuan, kelompok II diberi uranium 8 ppm dosis 0,01 mL/g BB sebagai kontrol negatif, kelompok III sebagai kontrol positif dengan vitamin C 200 mg/70 kg BB yang diberikan 15 menit sebelum pemajanan uranium, kelompok IV,V, dan VI sebagai kelompok uji proteksi kerusakan sel ginjal diberi infusa wortel (Daucus carota L.) berturut-turut 10%, 20%, 30% dengan dosis 0,01 mL/g BB 15 menit sebelum diberi uranium 8 ppm dosis 0,01 mL/g BB. Lima hari kemudian hewan dikorbankan dan diambil organ ginjalnya untuk dilakukan pemeriksaan makroskopis dan mikroskopis berupa pengamatan kondisi fisik organ ginjal, pengamatan terhadap nekrosis sel ginjal, dan skoring tipe kerusakan untuk menganalisis efek proteksinya. Hasil pemeriksaan histopatologi menyimpulkan bahwa infusa wortel dapat mengurangi kerusakan sel ginjal akibat paparan senyawa radioaktif uranium.Kidney is the vital organ which has an importance role in maintaining environmental stability of the body, with the main functions are glomerulus filtration, reabsorption and secretion by tubulus. The damage of kidney cells will reduce kidney function. If a kidney is exposed to radioactive substance it could deteriorate the kidney. The aim of this research was to find out the effect of carrot infusion used to prevent kidney cells disorder due to uranium exposure. Thirty rats wistar strain divided into 5 groups were used in this study, comprising of 5 rats in each groups throughout the experiment. Group I was a normal control group. Group II was given uranium of 8 ppm 0,01 mL/g BW as negative control group. Group III was given vitamin C of 200 mg/70 kgBW 15 minute prior to uranium exposure. Group IV-VI were given carrot infusion at 10%, 20%, 30% dose of 0,01 mL/g BW 15 minutes prior to uranium exposure. The rats were sacrificed in day-5, to observe the kidneys damage upon macroscopy and microscopy examination, involving physical condition of the kidney, necrosis of kidney cells and scoring the damage of kidney cells. The microscopic examination toward necrosis of kidney cells showed that carrot infusion can reduce cell damage due to radioactive compound of uranium

Active Cutaneous Anaphylaxis-inhibitory Activity of the N-hexane Extract of Morinda Citrifolia L. Fruits in Hepatitis B Vaccine-induced Wistar Rats

Noni (Morinda citrifolia L.) is one of medicinal plants that are commonly known for its medical treatment USAge. Noni is reported for the pharmacological effects, including analgesic, antihypertensive and immunomodulator. This research aims to prove that the n-hexane extract of noni fruits can inhibit active cutaneous anaphylaxis reaction in male wistar rats induced hepatitis B vaccine. This research used an active cutaneous anaphylaxis methode followed by hystopathological observation. Active cutaneous anaphylaxis test was done for 2 weeks. Test animals were divided into five groups: untreated control, treatment with n-hexane extract of noni dose of 50mg, 100mg dan 200mg/kgBW rats, and cromolyn treated group with dose of 2. mg/kg BW rats as positive control. Animals were sensitized 2times (once every week) with hepatitis B vaccine (dose of 145µg/kgBW) subcutaneously on the back. In the second week, random four rats of each groups were sacrificed and the skin tissues and livers were taken. Tissues were created as histopathology preparations with hematoxyllin-eosin coloring. The presence of anaphylaxis reactions, shown by inflammatory cell infiltration, were observed from the histopathology preparations. The result of this research showed that the administration of n-hexane extract of noni fruits treatment dose of 50mg, 100mg, and 200mg/kgBW on male Wistar rats induced hepatitis B vaccine can inhibit active cutaneous anaphylaxis reaction

Uji Aktivitas Imunomodulator Fermentasi Teh Hitam Jamur Kombucha Terhadap Fagositosis Makrofag Mencit Galur Balb/c Secara in Vitro

Black tea and kombucha is beverage drink was known that can increase immunity. This study aims to not only determine the fermented black tea kombucha as an immunomodulatory againts macrophages cell phagocytic of mice strains Balb/C in vitro, but also to determine concentration with duration fermentation that produces the highest activity Fermentation kombucha was carried out for 5, 10 and 15 days with steeping black tea has been given sugar. Fermented black tea kombucha was made to any series concentrations of 6,25; 12,5; 25; 50 and 100 µg/mL, as a positive control used lipopolysaccharide (LPS) 10 mg/mL there were tested in vitro on activity phagocytic macrophage cell of mice strains Balb/C. Value of Phagocytic Index (phagocytic of latex in 100 macrophage) and phagocytic capacity (amount of macrophage that phagocytic latex) were analyzed using SPSS 16 for windows with methode Friedman test followed by Mann Withney test. The results showed that fermented black tea kombucha has the highest as immunostimulatory activity on macrophage phagocytic at the concentration of 100 µg/mL and 10 days duration fermentation