Different forms of loop-mediated isothermal amplification (LAMP) for the detection of human viruses

Infections caused by viruses in humans can result in serious illnesses and even death. This means that early detection and diagnosis is extremely important for the control of these diseases. Currently, the loop-mediated isothermal amplification (LAMP) is regarded as a reliable method because as well as being cost-effective, simple and innovative, it allows nucleic acids to be amplified without the need for a large laboratory management structure. Thus, LAMP assay can be viewed as a highly efficient molecular-based advanced detection technique for several pathogens, including viruses. Systems based on LAMP have the following features: isothermal amplification, reproducibility and robustness, and they can be used when there is scarce or low quality genetic material. The amplification involves making use of, a maximum set of three specifically- designed primers and a DNA polymerase with strand-shifting activity. In most cases, this technique is far superior to other molecular techniques such as, for example, the conventional polymerase chain reaction (PCR) and its variants. The main advantage of the LAMP method is its cost-effectiveness, as it can be employed by means of a thermocycler, water bath or thermoblock. In this mini-review, information is provided about some of the main variants of the LAMP technique which have been designed for the diagnosis of several human viral pathogens

SHORT COMMUNICATION - Partial Molecular Characterization of Sm8, a Tegumental Antigen of Schistosoma mansoni

Sm8 is a major tegumental antigen of Schistosoma mansoni   . The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative leucine zipper pattern. The mRNA and the protein are predominantly expressed in adult worms

Humoral immune response of patients bitten by the snake Bothrops erythromelas

Submitted by Nuzia Santos ([email protected]) on 2012-11-06T13:41:52Z No. of bitstreams: 1 73.2010.pdf: 627949 bytes, checksum: e6f6124db34cf0f866fc505801a77c8b (MD5)Made available in DSpace on 2012-11-06T13:41:52Z (GMT). No. of bitstreams: 1 73.2010.pdf: 627949 bytes, checksum: e6f6124db34cf0f866fc505801a77c8b (MD5) Previous issue date: 2010State University of Paraíba. Center of Biological and Health Sciences. Departament of Biology. Campina Grande, PB, Brazil/ Fundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, Brazil.Fundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, BrazilFundação Oswaldo Cruz. Research Center René Rachou. Laboratory of Biomarkers and Monitoring. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Research Center René Rachou. Laboratory of Biomarkers and Monitoring. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, BrazilIntroducao: Envenenamentos ofídicos consistem problema de saúde pública em áreas rurais de países tropicais e subtropicais, mas pouco sabe-se sobre a resposta imune apresentada pelos indivíduos picados, por isso a avaliação da produção de IgM por pacientes picados por Bothrops erythromelas identificando a eficácia do tratamento nesse tipo de envenenamento. Metodos: O veneno de Bothrops erythromelas foi submetido a eletroforese e transferido para nitrocelulose, seguindo incubação com soro de pacientes. Resultados: Foi observada proteína de 38KDa antes e 24 horas após o tratamento. Conclusoes: Os resultados sugerem que essa proteína poderia ser utilizada como marcador para indivíduos envenenados pela serpente Bothrops erythromelas.Introduction: Snake envenomings are a health problem in rural áreas of tropical and subtropical countries, but little is known regarding the immune response presented by bitten individuals. The IgM production of patients bitten by Bothrops erythromelas snake was analyzed to identify the effectiveness of treatment in this type of envenomation. Methods: Bothrops erythromelas venom was submitted to electrophoresis and transferred to a nitrocellulose sheet, following incubation with patients’ sera. Results: A 38 KDa protein was detected before and 24 h after therapy. Conclusions: The result suggests that this protein could be used as a marker for individuals envenomed by Bothrops. erythromelas