SHORT COMMUNICATION - Partial Molecular Characterization of Sm8, a Tegumental Antigen of Schistosoma mansoni

Sm8 is a major tegumental antigen of Schistosoma mansoni   . The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative leucine zipper pattern. The mRNA and the protein are predominantly expressed in adult worms

Humoral immune response of patients bitten by the snake Bothrops erythromelas

Submitted by Nuzia Santos ([email protected]) on 2012-11-06T13:41:52Z No. of bitstreams: 1 73.2010.pdf: 627949 bytes, checksum: e6f6124db34cf0f866fc505801a77c8b (MD5)Made available in DSpace on 2012-11-06T13:41:52Z (GMT). No. of bitstreams: 1 73.2010.pdf: 627949 bytes, checksum: e6f6124db34cf0f866fc505801a77c8b (MD5) Previous issue date: 2010State University of Paraíba. Center of Biological and Health Sciences. Departament of Biology. Campina Grande, PB, Brazil/ Fundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, Brazil.Fundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, BrazilFundação Oswaldo Cruz. Research Center René Rachou. Laboratory of Biomarkers and Monitoring. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Research Center René Rachou. Laboratory of Biomarkers and Monitoring. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Research Center Aggeu Magalhães. Departament of Immunology Recife, PE, BrazilIntroducao: Envenenamentos ofídicos consistem problema de saúde pública em áreas rurais de países tropicais e subtropicais, mas pouco sabe-se sobre a resposta imune apresentada pelos indivíduos picados, por isso a avaliação da produção de IgM por pacientes picados por Bothrops erythromelas identificando a eficácia do tratamento nesse tipo de envenenamento. Metodos: O veneno de Bothrops erythromelas foi submetido a eletroforese e transferido para nitrocelulose, seguindo incubação com soro de pacientes. Resultados: Foi observada proteína de 38KDa antes e 24 horas após o tratamento. Conclusoes: Os resultados sugerem que essa proteína poderia ser utilizada como marcador para indivíduos envenenados pela serpente Bothrops erythromelas.Introduction: Snake envenomings are a health problem in rural áreas of tropical and subtropical countries, but little is known regarding the immune response presented by bitten individuals. The IgM production of patients bitten by Bothrops erythromelas snake was analyzed to identify the effectiveness of treatment in this type of envenomation. Methods: Bothrops erythromelas venom was submitted to electrophoresis and transferred to a nitrocellulose sheet, following incubation with patients’ sera. Results: A 38 KDa protein was detected before and 24 h after therapy. Conclusions: The result suggests that this protein could be used as a marker for individuals envenomed by Bothrops. erythromelas