6,443 research outputs found
An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs
For the sensitive detection of eggs of Echinococcus multilocularis in fox faeces by PCR we have evaluated a method based on the previous concentration of helminth eggs by a combination of sequential sieving of faecal samples and flotation of the eggs in zinc chloride solution. The eggs were microscopically detected in the fractions retained in 40 and 20µm mesh sieves. DNA of the taeniid eggs retained in the 20 µm sieve was obtained after alkaline lysis and PCR was performed using E. multilocularis species-specific primers. Compared to the parasitological findings after examination of the small intestines of the foxes, the specificity of the PCR was 100% (no false-positive result with 20 foxes free of E. multilocularis) and the sensitivity was 94% (33 positive results from total 35 foxes proven to be infected with E. multilocularis). Both false-negative results were obtained with faeces from foxes harbouring immature worms. Using faecal volumes between 2 and 20 ml, no inhibition of PCR was observed as was demonstrated by the amplification of size-modified target in parallel reactions. The tests were undertaken with fresh faeces stored in 70% ethanol, but egg detection by PCR was also possible after inactivation of eggs by freezing the faeces at −80°C for one week or by incubation at +70°C for 2
Entanglement in SO(3)-invariant bipartite quantum systems
The structure of the state spaces of bipartite (N tensor N) quantum systems
which are invariant under product representations of the group SO(3) of
three-dimensional proper rotations is analyzed. The subsystems represent
particles of arbitrary spin j which transform according to an irreducible
representation of the rotation group. A positive map theta is introduced which
describes the time reversal symmetry of the local states and which is unitarily
equivalent to the transposition of matrices. It is shown that the partial time
reversal transformation theta_2 = (I tensor theta) acting on the composite
system can be expressed in terms of the invariant 6-j symbols introduced by
Wigner into the quantum theory of angular momentum. This fact enables a
complete geometrical construction of the manifold of states with positive
partial transposition and of the sets of separable and entangled states of (4
tensor 4) systems. The separable states are shown to form a three-dimensional
prism and a three-dimensional manifold of bound entangled states is identified.
A positive maps is obtained which yields, together with the time reversal, a
necessary and sufficient condition for the separability of states of (4 tensor
4) systems. The relations to the reduction criterion and to the recently
proposed cross norm criterion for separability are discussed.Comment: 15 pages, 3 figure
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Comparative study of design: application to Engineering Design
A recent exploratory study examines design processes across domains and compares them. This is achieved through a series of interdisciplinary, participative workshops. A systematic framework is used to collect data from expert witnesses who are practising designers across domains from engineering through architecture to product design and fashion, including film production, pharmaceutical drugs, food, packaging, graphics and multimedia and software. Similarities and differences across domains are described which indicate the types of comparative analysis we have been able to do from our data. The paper goes further and speculates on possible lessons for selected areas of engineering design which can be drawn from comparison with processes in other domains. As such this comparative design study offers the potential for improving engineering design processes. More generally it is a first step in creating a discipline of comparative design which aims to provide a new rich picture of design processes
Reentrant glass transition in a colloid-polymer mixture with depletion attractions
Performing light scattering experiments we show that introducing short-ranged
attraction to a colloidal suspension of nearly hard spheres by addition of free
polymer produces new glass transition phenomena. We observe a dramatic
acceleration of the density fluctuations amounting to the melting of a
colloidal glass. Increasing the strength of the attractions the system freezes
into another nonergodic state sharing some qualitative features with gel states
occurring at lower colloid packing fractions. This reentrant glass transition
is in qualitative agreement with recent theoretical predictions.Comment: 14 pages, 3 figure
The interaction of class and gender in illness narratives
This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2008 BSA Publications Ltd.Perspectives on gender and identity that emphasize variability of performance, local context and individual agency have displaced earlier paradigms.These are now perceived to have supported gender stereotypes and language ideologies by emphasizing gender difference and homogeneity within genders. In a secondary analysis of health and illness narratives we explore the interaction of class and gender in individuals' constructions of gendered identity. High social class men perform gender in particularly varied ways and we speculate that this variable repertoire, including the use of what was once termed `women's language', is linked to a capacity to maintain social distinction and authority. Men's performance of conventional masculinity is often threatened by both the experience of illness and being interviewed about personal experience. Lower social class women in particular demonstrate an intensification of a pre-existing informal family and support group culture, marking successful members by awarding them the accolade of being `lovely'.ESR
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