Manipulation of lipid rafts in neuronal cells

Lipid rafts are specialized plasma membrane micro-domains highly enriched in cholesterol, sphingolipids and glycosylphosphatidylinositol (GPI) anchored proteins. Lipid rafts are thought to be located in the exofacial leaflet of plasma membranes. Functionally, lipid rafts are involved in intracellular trafficking of proteins and lipids, secretory and endocytotic pathways, signal transduction, inflammation and in cell-surface proteolysis. There has been substantial interest in lipid rafts in brain, both with respect to normal functioning and with certain neurodegenerative diseases. Based on the impact of lipid rafts on multitude biochemical pathways, modulation of lipid rafts is used to study related disease pathways and probably offers a target for pharmacological intervention. Lipid rafts can be targeted by modulation of its main components, namely cholesterol and sphingolipids. Other approaches include the modulation of membrane dynamics and it has been reported that protein-lipid interactions can vary the occurrence and composition of these membrane micro-domains. The present review summarizes the possibilities to modulate lipid rafts with focus on neuronal cells. Keywords: Lipid raft, cholesterol, membrane fluidity, statin, cyclodextrine, docosahexaenoic acid

The reality of design process planning

Most companies struggle with the efficiency of their processes. One contributory factor is the lack of efficient process planning. This paper describes current planning practise in industry, which uses a multitude of different plans in parallel. The units of planning and their resulting plans roughly fall into product plans considering cost, bill of material and procurement considerations; process plans including different milestone, task and activity plans and quality plans. This paper maps out the ownership of these plans, and establishes that organisations work because individuals use more then one plan and have a tacit understanding of the relationships between these plans. The lack of effective plans affects the company through a lack of understanding of process connectivity and in consequence bad communication

NASA metric system study

Statistical analysis of metric and English unit usage and trends in technical documents generated by NASA Center

Towards a change process planning tool

The relationship between a product and its design process is generally complex and not fully understood. When modifying a product, industry still rarely considers the implementation process and its consequences for other design activities in the company, which is hard to assess with conventional planning methods. Although change processes are highly constrained, product and process constraints are not usually considered together or traded off against each other when planning the change. Inadequate assessment and planning of the change implementation process can lead to costly knock-on effects across the product and the design process. This paper argues for a combination of change and process research and discusses requirements for a change process planning tool. It proposes a system for the analysis of the impact of change on the product as well as other company activities. Then, a more informed selection between change alternatives is possible

Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations

A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens, respectively. In experimentally infected dogs and cats, coproantigens were first detectable 6-17 days postinfection (PI) in samples of 8 dogs (worm burdens at necropsy: 6,330-43,200) and from 11 days PI onward in samples of 5 cats infected with 20-6,833 worms. After anthelmintic treatment of 4 dogs and 5 cats at day 20 PI, coproantigen excretion disappeared within 3-5 days. The sensitivity of the ELISA was 83.6% in 55 foxes infected with 4-60,000 E. multilocularis, but reached 93.3% in the 45 foxes harboring more than 20 worms. The EM-ELISA was used in surveys of 'normal' dog and cat populations in Switzerland. Among 660 dogs and 263 cats, 5 dogs and 2 cats exhibited a positive reaction. In 2 of these dogs (0.30%) and 1 cat (0.38%), intestinal E. multilocularis infections were confirmed by necropsy, polymerase chain reaction PCR, or both. The specificities of the ELISA in these groups were found to be 99.5% and 99.6%, respectively, if positive ELISA results that could not be confirmed by other methods were classified as 'false positive' reactions

The spiral of applied research: A methodological view on integrated design research

Abstract not available

Model granularity and related concepts

Models are integral to engineering design and basis for many decisions. Therefore, it is necessary to comprehend how a model’s properties might influence its behaviour. Model granularity is an important property but has so far only received limited attention. The terminology used to describe granularity and related phenomena varies and pertinent concepts are distributed across communities. This article positions granularity in the theoretical background of models, collects formal definitions for relevant terms from a range of communities and discusses the implications for engineering design

Parameter trails

Successful communication is vital for the success of any design project. However, communication often fails, adversely affecting design process efficiency and product quality.understand the connections between different aspects of design and don–t know where to find out more information or who to talk to. This paper presents a new model, developed from current project planning techniques, which supports communication using parameter-specific data. It enables designers to question information, inform their colleagues pro-actively and assess the impact of changing parameter values on subsequent design tasks. Such interaction is critical in allowing designers to see how their own tasks fit into the overall product design