Effects of combination of ethylenediaminetetraacetic acid and microbial phytase on the serum concentration and digestibility of some minerals in broiler chicks

This experiment was conducted to evaluate the combined effects of ethylene diamine tetraacetic acid (EDTA) and microbial phytase (MP) on the serum concentration and digestibility of some minerals in broiler chicks. This experiment was conducted using 360 Ross-308 male broiler chicks in a completely randomized design with a 3×2 factorial arrangement (0, 0.1 and 0.2% EDTA and 0 and 500 FTU MP). Fourreplicates of 15 chicks per each were fed dietary treatments which included; P-deficient basal diet [0.2% available phosphorus (aP)] (NC), NC + 500 FTU MP per kilogram of diet, NC + 0.1% EDTA per kilogram ofdiet, NC + 0.1% EDTA + 500 FTU MP per kilogram of diet, NC + 0.2% EDTA per kilogram and NC + 0.2% EDTA + 500 FTU MP per kilogram of diet. The concentration of zinc, copper and manganese of serum and their digestibility and also digestibility of apparent metabolizable energy (AMEn) was evaluated. The results showed that phytase supplementation of P-deficient diets significantly increased zinc concentration of serum (P < 0.05). Interaction effect of EDTA+MP on serum concentration of copper and manganese and also digestibility of zinc was significant (P < 0.05). EDTA supplementation of P-deficient diets significantly increased manganese digestibility in broiler chicks (P < 0.01). Keywords: Ethylene diamine tetraacetic acid, microbial phytase, zinc, copper, manganes

Effects of dietary inclusion of commercial toxin binders and prebiotics on performance and immune responses of broiler chicks fed aflatoxin-contaminated diets

This study aimed to evaluate the efficiency of the dietary inclusion of commercial toxin binders (CTs) and prebiotics on growth performance, immune responses, intestinal morphology and blood variables of broiler chicks fed with aflatoxin and non-aflatoxin-contaminated diets. Six hundred one-day-old male Ross 308 broiler chicks, initial weight of 42 ± 3 g, were used in 10 treatments with six replications (n = 10 birds). ASRI1 and ASRI2 commercial toxin binders and lactose prebiotic were included in their diets. Experimental diets included: 1) basal diet without aflatoxin and additives (NC); 2) basal diet containing aflatoxin (PC); 3) NC diet containing ARSI1; 4) NC diet containing ARSI2; 5) NC diet containing prebiotics; 6) PC diet containing ARSI1; 7) PC diet containing ARSI2; 8) PC diet containing prebiotics; 9) PC diet containing ARSI1+prebiotics; and 10) PC diet containing ARSI2+prebiotics. Growth performance, humoral and cellular immune responses, jejunal morphology and some blood variables were assessed. Results showed that broiler chicks fed with a PC diet showed a higher feed conversion ratio and lower body weight in the grower and finisher periods. Broiler chicks fed with PC diets showed lower immunoglobulin G and M and also cellular immunities compared to the NC diet. The PC group also showed lower values for villus length, villus width and crypt depth, and higher values for liver enzyme activities compared to the PC diet. However, dietary inclusion of prebiotics and CTs, in single and combined form, improved growth performance in grower and finisher periods, cellular and humoral immunities, intestinal morphology and the serum concentration of triglycerides, cholesterol, glucose and liver enzyme activities of broiler chicks fed with aflatoxin.Keywords: Aflatoxin, ASRI1 toxin binder, broiler chicks, growth performance, humoral immunit

Personalized peptide-based vaccination for treatment of colorectal cancer: rational and progress

Colorectal cancer (CRC) is one of the most common cancers globally and is associated with a high rate of morbidity and mortality. A large proportion of patients with early stage CRC who undergo conventional treatments develop local recurrence or distant metastasis and in this group of advanced disease, the survival rate is low. Furthermore there is often a poor response and/or toxicity associated with chemotherapy and chemo-resistance may limit continuing conventional treatment alone. Choosing novel and targeted therapeutic approaches based on clinicopathological and molecular features of tumors in combination with conventional therapeutic approach could be used to eradicate residual micrometastasis and therefore improve patient prognosis and also be used preventively. Peptide-based vaccination therapy is one class of cancer treatment that could be used to induce tumor-specific immune responses, through the recognition of specific antigen-derived peptides in tumor cells, and this has emerged as a promising anti-cancer therapeutic strategy. The aim of this review was to summarize the main findings of recent studies in exciting field of peptide-based vaccination therapy in CRC patients as a novel therapeutic approach in treatment of CRC

The effect of different levels of diet total volatile nitrogen on performance, carcass characteristics and meat total volatile nitrogen in broiler chickens

This study was conducted to determine the effect of different levels of diet total volatile nitrogen (TVN) on performance, carcass characteristics and meat TVN in broiler chickens. A total of 400 one-day-old Ross 308 broiler chicks was used in this study. On the first day, male and female chicks were separated by feather sexing. In the first week, all chicks were reared together and fed with a basal diet without urea. At the beginning of the second week, the male and female chicks were weighed so that the average body weight of chicks was approximately equal in each cage. Then the male and female chicks were allocated to 25 floor pens in a completely randomized design with five treatments, five replicates and 16 chicks in each replicate (eight males and eight females) throughout the experimental period, which lasted for 42 days. Dietary treatments consisted of zero (control), 0.5, 1, 1.5 and 2 % of urea in the diets. Before starting the experiment, the TVN levels in all diets were measured after adding different levels of urea, and TVN levels were 13.30, 14.95, 17.26, 23.26 and 27.47 mg 100 g^&minus;1 and 16.66, 15.02, 17.81, 24.66 and 26.25 mg 100 g^&minus;1 in starter and grower diets, respectively. Feed intake (FI), body weight gain (BWG) and the feed conversion ratio (FCR) were measured. Carcass characteristics as well as TVN in breast meat, thigh meat and the whole carcass and in left tibia and toe ash were measured. The results showed that FI levels were significantly different between the groups at different weeks except for the second week (<i>P</i> &lt; 0.05). The BWG significantly differed among treatments (<i>P</i> &lt; 0.05). In the second and sixth weeks, FCR was affected by increasing TVN in the diet (<i>P</i> &lt; 0.05). Increasing TVN in broiler diets had a significant effect on the carcass characteristics (<i>P</i> &lt; 0.05). Increasing TVN to more than 15 mg 100 g^&minus;1 linearly reduced left tibia and toe ash (<i>P</i> &lt; 0.05). In addition, increasing TVN in the broiler diets affected the TVN of breast meat, thigh meat and the whole carcass of broilers (<i>P</i> &lt; 0.05). These results suggest that increasing diet TVN to more than 15 mg 100 g^&minus;1 reduces performance traits, carcass characteristics, and left tibia and toe ash and increases the TVN amount of breast meat, thigh meat and the whole carcass

Evaluation of Probiotic Potential of Some Native Lactobacillus Strains on the Growth Performance and Serum Biochemical Parameters of Japanese Quails (Coturnix Coturnix Japonica) during Rearing Period

ABSTRACT The objective of the present study was to evaluate the probiotic effects of different concentrations of four selected native Lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails. A completely randomized design (CRD) was applied, including seven probiotic treatments with four replicates of 20 quails each, totaling 560 quails. Treatments were applied for five weeks. Four native Lactobacillus strains were anaerobically grown in a 10-L batch fermenter and lyophilized (1010 CFU/g). Treatments were as follows: T1: control (basal diet); T2: commercial probiotic CP1; T3: commercial probiotic CP2; and T4, T5, T6, and T7: four native strains added at levels of 50, 100, 150, and 200 g/ton diet, respectively. The native probiotics significantly improved body weight gain (BWG) and feed conversion ratio (FCR) during the starter, finisher, and overall periods (35 days) (p0.05) on blood cholesterol, calcium, high-density lipoprotein (HDL), or hemoglobin (HB) levels or on red blood cell counts (RBC). The cecal and small intestine samples of the quails fed the native Lactobacillus strains contained significantly higher Lactobacillus spp. and lower E. coli populations compared with the control diet and those supplemented with commercial probiotics. It was concluded that the use of the native Lactobacillus strains (150 g/ton diet) promoted the best performance of Japanese quails

The effects of in ovo feeding of glutamine in broiler breeder eggs on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens

The aim of the present study was to investigate the effect of in ovo feeding (IOF) of glutamine on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens. Fertilized eggs were subjected to injections with glutamine (Gln) (10, 20, 30, 40 or 50 mg dissolved in 0.5 mL of dionized water) on day 7 of incubation. Hatchability, growth performance, carcass characteristics (carcass weight and relative weights of breast, thigh, heart, liver, gizzard, abdominal fat, intestine, pancreas and spleen) and jejunal morphometry (measurement of villus height and width and crypt depth) were determined during the experiment. The weight of newly hatched chickens was significantly greater in groups with Gln injection than in control and sham groups. But IOF caused lower hatchability than in the control group (non-injected eggs) (<i>p</i> &lt; 0.05). Chickens from IOF of Gln showed better weight gain and feed conversion ratio (0&ndash;42 days of age), when compared to chickens hatched from control and sham groups. The IOF of Gln significantly increased villus height, villus width and crypt depth at hatch period and villus height at 42 days of age. In addition, carcass weights and relative weights of breast, thigh and gizzard were also markedly increased in chickens treated in ovo with Gln; whereas heart, liver, abdominal fat, intestine, pancreas and spleen were not significantly altered at the end of the experimental period. These data suggest that the IOF of Gln may improve jejunum development, leading to an increased nutrient assimilation and consequently to greater performance in broiler chickens

Effects of Organic Acids Supplement on Performance, Egg Traits, Blood Serum Biochemical Parameters and Gut Microflora in Female Japanese Quail (Coturnix coturnix japonica)

ABSTRACT This study was conducted to evaluate the effects of acetic acid, lactic acid, and butyric acid on the production performance, egg parameters (quality and quantity traits), blood and liver serum biochemical parameters and gastrointestinal tract microorganism on female Japanese quails. A total of 640 female Japanese quails aged 35-84 days were housedfor eight treatments with four replicates. They were arranged in completely randomized design (CRD) to evaluate the effects of basal diet (BD) with a supplement of acetic acid (AA), lactic acid (LA) and butyric acid (BA). The performance parameters to study this effect are feed intake, feed conversion ratio, egg traits such as egg weight, egg production, and egg mass along with specific gravity, internal quality unit (IQU), yolk and albumen indexes, and eggshell traits such as weight, thickness, and surface. It also includes blood serum biochemical parameters such as cholesterol, triglyceride, HDL, LDL, albumin, globulin, glucose, and gastrointestinal tract microflora (E.Coli and Salmonella). Eight dietary treatments were formulated by addition of organic acids such as BD without organic acids additive, BD with 124 mg/kg AA additive, BD with 104 mg/kg LA additive, BD with 113 mg/kg BA additive, BD with 63.5 mg/kg AA+53.5 mg/kg LA additive, BD with 63.5 mg/kg AA+57 mg/kg BA additive, BD with 53.5 mg/kg LA+57 mg/kg BA additive and BD with 41.6 mg/kg AA+35 mg/kg LA+37 mg/kg BA additive. The results showed that the diets containing BD+LA+BA+AA and BD+AA alone considerably increased feed intake, feed conversion ratio, egg weight, egg production and egg mass (p<0.01). The diets containing BD+AA and BD+LA significantly affected some quality traits such as eggshell thickness, eggshell weight (p<0.01), shell surface, and IQU (p<0.05). The diets containing BD+AA and BD+LA alone increased serum cholesterol, HDL, albumin, globulin and total protein significantly (p<0.01). Moreover, the groups containing BD+AA and BD+LA significantly decreased triglyceride contents in serum (p<0.05). The results showed that the diets containing BD+LA and BD+BA significantly decreased the E.Coli and Salmonella population in the gastrointestinal tract (p<0.01). Based on the experiment, it can beinferred that LA alone, or in combination with other organic acids, improved and showed positive effects on performance and egg traits in female Japanese quail

Effects of Organic Acids Supplement on Performance, Egg Traits, Blood Serum Biochemical Parameters and Gut Microflora in Female Japanese Quail (Coturnix coturnix japonica)

<div><p>ABSTRACT This study was conducted to evaluate the effects of acetic acid, lactic acid, and butyric acid on the production performance, egg parameters (quality and quantity traits), blood and liver serum biochemical parameters and gastrointestinal tract microorganism on female Japanese quails. A total of 640 female Japanese quails aged 35-84 days were housedfor eight treatments with four replicates. They were arranged in completely randomized design (CRD) to evaluate the effects of basal diet (BD) with a supplement of acetic acid (AA), lactic acid (LA) and butyric acid (BA). The performance parameters to study this effect are feed intake, feed conversion ratio, egg traits such as egg weight, egg production, and egg mass along with specific gravity, internal quality unit (IQU), yolk and albumen indexes, and eggshell traits such as weight, thickness, and surface. It also includes blood serum biochemical parameters such as cholesterol, triglyceride, HDL, LDL, albumin, globulin, glucose, and gastrointestinal tract microflora (E.Coli and Salmonella). Eight dietary treatments were formulated by addition of organic acids such as BD without organic acids additive, BD with 124 mg/kg AA additive, BD with 104 mg/kg LA additive, BD with 113 mg/kg BA additive, BD with 63.5 mg/kg AA+53.5 mg/kg LA additive, BD with 63.5 mg/kg AA+57 mg/kg BA additive, BD with 53.5 mg/kg LA+57 mg/kg BA additive and BD with 41.6 mg/kg AA+35 mg/kg LA+37 mg/kg BA additive. The results showed that the diets containing BD+LA+BA+AA and BD+AA alone considerably increased feed intake, feed conversion ratio, egg weight, egg production and egg mass (p<0.01). The diets containing BD+AA and BD+LA significantly affected some quality traits such as eggshell thickness, eggshell weight (p<0.01), shell surface, and IQU (p<0.05). The diets containing BD+AA and BD+LA alone increased serum cholesterol, HDL, albumin, globulin and total protein significantly (p<0.01). Moreover, the groups containing BD+AA and BD+LA significantly decreased triglyceride contents in serum (p<0.05). The results showed that the diets containing BD+LA and BD+BA significantly decreased the E.Coli and Salmonella population in the gastrointestinal tract (p<0.01). Based on the experiment, it can beinferred that LA alone, or in combination with other organic acids, improved and showed positive effects on performance and egg traits in female Japanese quail.</p></div