1,277 research outputs found
Genetically Engineered Peptides for Light Harvesting and Folding Kinetics Studies
The preparation and characterization of ÎČ-sheet forming polypeptides for use in light harvesting applications is described. The Higashiya genetic cassette strategy (HGCS) was employed in order to make DNA libraries of repetitive DNA sequences. These DNA libraries contained gene cassettes coding for proteins designed to fold into the desired secondary motif. The conditions required for peptide expression, folding and formation of micron length fibrils was determined for the purpose of photophysical characterization, such as fluorescence lifetimes and acceptor dye quantum yields. Of special interest was the investigation of an âantenna effectâ under FRET illumination, the intensity of acceptor dye emission was examined as a consequence of increasing peptide length. When ÎČ-sheet forming polypeptides bearing tryptophan residues along one edge were irradiated at 280 nm, the tryptophan absorbance maximum, the fluorescence intensity of the C-terminal acceptor chromophore, N-(7-dimethylamino-4-methyl) coumarin (DACM), increased proportionally with an increasing number of donor tryptophans. The fluorescence intensity increased even as the length of the ÎČ-sheet edge approached 256 Ă
, well beyond the Förster radius for the tryptophan-acceptor chromophore pair. Folding of the polypeptides was examined as well using Circular Dichroism, and fibrillation was examined using ThT kinetic studies. Beta sheet formation was detected via Circular Dichroism, and fibrillation was confirmed by the ThT kinetics studies. The plots of CPS vs. time showed an increase in florescence which was indicated by the positive slope of the plot, this positive slope shows that fibrillation is occurring
Movements and Area Use of Belugas, Delphinapterus leucas, in a Subarctic Alaskan Estuary
Seasonal movements of 14 belugas in Cook Inlet, Alaska, were monitored by satellite telemetry between July and March in 2000â03. Whales used waters in the upper Cook Inlet intensively between summer and late autumn and dispersed to mid-inlet offshore waters during winter months. All whales remained in Cook Inlet the entire time they were tracked, and several whales were tracked through March. During summer and early fall, movements were clearly concentrated in specific areas, generally river mouths or bays, where whales were likely feeding on fish runs. Average daily travel distances ranged from 11 to 30 km per day. Monthly home ranges, estimated using the 95% kernel probability distribution of average daily positions, were smallest in August (982 km2), increased throughout autumn, and peaked in winter (reaching approximately 5000 km2). The seasonal variation in distribution and movement patterns displayed by belugas in Cook Inlet affect the sighting rates and seasonal abundance estimates obtained for this depleted population.Les dĂ©placements saisonniers de 14 bĂ©lugas du dĂ©troit de Cook, en Alaska, ont fait lâobjet dâune surveillance au moyen dâun Ă©metteur par satellite entre les mois de juillet et mars 2000 Ă 2003. Cela a permis de remarquer que les baleines se tenaient beaucoup dans les eaux de la partie supĂ©rieure du dĂ©troit de Cook de lâĂ©tĂ© jusquâĂ la fin de lâautomne, mais quâelles se dispersaient dans les eaux du large du milieu du dĂ©troit pendant les mois dâhiver. Toutes les baleines sont restĂ©es dans le dĂ©troit de Cook pendant toute la durĂ©e de surveillance, et plusieurs baleines ont Ă©tĂ© suivies jusquâau mois de mars. LâĂ©tĂ© et au dĂ©but de lâautomne, les dĂ©placements Ă©taient nettement concentrĂ©s dans des endroits spĂ©cifiques, gĂ©nĂ©ralement dans les embouchures ou les baies, oĂč les baleines se nourrissaient probablement de poissons. En moyenne, les baleines se dĂ©plaçaient sur des distances variant de 11 Ă 30 km par jour. Câest en aoĂ»t que le domaine vital mensuel, estimĂ© par la mĂ©thode du noyau en fonction dâune densitĂ© de probabilitĂ© de 95 % des positions quotidiennes moyennes, Ă©tait le plus petit (982 km2), aprĂšs quoi il augmentait Ă lâautomne et culminait lâhiver (oĂč il atteignait environ 5 000 km2). La variation saisonniĂšre caractĂ©risant la rĂ©partition et les dĂ©placements des bĂ©lugas dans le dĂ©troit de Cook exerce une influence sur le taux dâobservations et sur les estimations dâabondance saisonniĂšre obtenues pour cette population en dĂ©clin
The Body Dances: Carnival Dance and Organization
Building on the work of Pierre Bourdieu and Maurice Merleau-Ponty we seek to open up traditional categories of thought surrounding the relation `body-organization' and elicit a thought experiment: What happens if we move the body from the periphery to the centre? We pass the interlocking theoretical concepts of object-body/subject-body and habitus through the theoretically constructed empirical case of `carnival dance' in order to re-evaluate such key organizational concepts as knowledge and learning. In doing so, we connect with an emerging body of literature on `sensible knowledge'; knowledge that is produced and preserved within bodily practices. The investigation of habitual appropriation in carnival dance also allows us to make links between repetition and experimentation, and reflect on the mechanism through which the principles of social organization, whilst internalized and experienced as natural, are embodied so that humans are capable of spontaneously generating an infinite array of appropriate actions. This perspective on social and organizational life, where change and permanence are intricately interwoven, contrasts sharply with the dominant view in organization studies which juxtaposes change/ creativity and stability
Kant's philosophy of the aesthetic and the philosophy of praxis
This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2012 Association for Economic and Social Analysis.This essay seeks to reconstruct the terms for a more productive engagement with Kant than is typical within contemporary academic cultural Marxism, which sees him as the cornerstone of a bourgeois model of the aesthetic. The essay argues that, in the Critique of Judgment, the aesthetic stands in as a substitute for the missing realm of human praxis. This argument is developed in relation to Kant's concept of reflective judgment that is in turn related to a methodological shift toward inductive and analogical procedures that help Kant overcome the dualisms of the first two Critiques. This reassessment of Kant's aesthetic is further clarified by comparing it with and offering a critique of Terry Eagleton's assessment of the Kantian aesthetic as synonymous with ideology
PVSS13. Acute Mesenteric Ischemia: A Comparison of Endovascular Revascularization to Traditional Therapy
Comparison of indirect radiation dose estimates with directly measured radiation dose for patients and operators during complex endovascular procedures
BackgroundA great deal of attention has been directed at the necessity and potential for deleterious outcomes as a result of radiation exposure during diagnostic evaluations and interventional procedures. We embarked on this study in an attempt to accurately determine the amount of radiation exposure given to patients undergoing complex endovascular aortic repair. These measured doses were then correlated with radiation dose estimates provided by the imaging equipment manufacturers that are typically used for documentation and analysis of radiation-induced risk.MethodsConsecutive patients undergoing endovascular thoracoabdominal aneurysm (eTAAA) repair were prospectively studied with respect to radiation dose. Indirect parameters as cumulative air kerma (CAK), kerma area product (KAP), and fluoroscopy time (FT) were recorded concurrently with direct measurements of dose (peak skin dose [PSD]) and radiation exposure patterns using radiochromatic film placed in the back of the patient during the procedure. Simultaneously, operator exposure was determined using high-sensitivity electronic dosimeters. Correlation between the indirect and direct parameters was calculated. The observed radiation exposure pattern was reproduced in phantoms with over 200 dosimeters located in mock organs, and effective dose has been calculated in an in vitro study. Scatter plots were used to evaluate the relationship between continuous variables and Pearson coefficients.ResultseTAAA repair was performed in 54 patients over 5 months, of which 47 had the repair limited to the thoracoabdominal segment. Clinical follow-up was complete in 98% of the patients. No patients had evidence of radiation-induced skin injury. CAK exceeded 15 Gy in 3 patients (the Joint Commission on Accreditation of Healthcare Organizations [JCAHO] threshold for sentinel events); however, the direct measurements were well below 15 Gy in all patients. PSD was measured by quantifying the exposure of the radiochromatic film. PSD correlated weakly with FT but better with CAK and KAP (r = 0.55, 0.80, and 0.76, respectively). The following formula provides the best estimate of actual PSD = 0.677 + 0.257 CAK. The average effective dose was 119.68 mSv (for type II or III eTAAA) and 76.46 mSv (type IV eTAAA). The operator effective dose averaged 0.17 mSv/case and correlated best with the KAP (r = 0.82, P < .0001).ConclusionFT cannot be used to estimate PSD, and CAK and KAP represent poor surrogate markers for JCAHO-defined sentinel events. Even when directly measured PSDs were used, there was a poor correlation with clinical event (no skin injuries with an average PSD >2 Gy). The effective radiation dose of an eTAAA is equivalent to two preoperative computed tomography scans. The maximal operator exposure is 50 mSv/year, thus, a single operator could perform up to 294 eTAAA procedures annually before reaching the recommended maximum operator dose
Documentos e Debates: RĂ©plica 1 â ClĂĄssicos & Formação em Administração: O Que, Quando, Para Que
Epitaxial Self-Assembly of Interfaces of 2D MetalâOrganic Frameworks for Electroanalytical Detection of Neurotransmitters
This paper identifies the electrochemical properties of individual facets of anisotropic layered conductive metalâorganic frameworks (MOFs) based on M3(2,3,6,7,10,11-hexahydroxytriphenylene)2 (M3(HHTP)2) (M = Co, Ni). The electroanalytical advantages of each facet are then applied toward the electrochemical detection of neurochemicals. By employing epitaxially controlled deposition of M3(HHTP)2 MOFs on electrodes, the contribution of the basal plane ({001} facets) and edge sites ({100} facets) of these MOFs can be individually determined using electrochemical characterization techniques. Despite having a lower observed heterogeneous electron transfer rate constant, the {001} facets of the M3(HHTP)2 systems prove more selective and sensitive for the detection of dopamine than the {100} facets of the same MOF, with the limit of detection (LOD) of 9.9 ± 2 nM in phosphate-buffered saline and 214 ± 48 nM in a simulated cerebrospinal fluid. Langmuir isotherm studies accompanied by all-atom MD simulations suggested that the observed improvement in performance and selectivity is related to the adsorption characteristics of analytes on the basal plane versus edge sites of the MOF interfaces. This work establishes that the distinct crystallographic facets of 2D MOFs can be used to control the fundamental interactions between analyte and electrode, leading to tunable electrochemical properties by controlling their preferential orientation through self-assembly
Perioperative cardiac events in endovascular repair of complex aortic aneurysms and association with preoperative studies
BackgroundEndovascular repair of complex aortic aneurysms (CAAs) can be performed in high-risk individuals, yet is still associated with significant morbidity, including spinal cord ischemia, cardiac complications, and death. This analysis was undertaken to better define the cardiac risk for CAA.MethodsA prospective database of patients undergoing thoracoabdominal or juxtarenal aortic aneurysm repair with branched and fenestrated endografts was used to retrospectively determine the number of cardiac events, defined as myocardial infarction (MI), atrial fibrillation (AF), and ventricular arrhythmia (VA), that occurred â€30 days of surgery. Postoperative serial troponin measurements were performed in 266 patients. Any additional available cardiac information, including preoperative echocardiography, physiologic stress tests, and history of cardiac disease, was obtained from medical records. The efficacy of preoperative stress testing and the association of various echo parameters were evaluated in the context of cardiac outcomes using univariable and multivariable logistic regression models.ResultsBetween August 2001 and December 2007, 395 patients underwent endovascular repair of a thoracoabdominal or juxtarenal aortic aneurysm. The incidence of AF, VA, and 30-day cardiac-related death was 9%, 3%, and 2%, respectively. Overall 30-day mortality was 6%. Univariable analysis showed the presence of mitral annulus calcification was associated with MI (odds ratio [OR], 3.5; 95% confidence interval [CI], 0.9-13.8; P = .07). Left atrium cavity area, ejection fraction, left ventricle mass, and left ventricular mass index were univariably associated with the presence of VA. Multivariable analysis showed only the left atrium cavity area was independently associated with VA (OR, 1.2; 95% CI, 1.0-1.5; P = .07). Stress test was done in 179 patients. Negative stress test results occurred in 152 (85%), of whom 9 (6%) sustained an MI during the 30-day perioperative course. MI occurred in 2 of the 27 patients (7%) who had a positive stress test result.ConclusionsEndovascular repair of CAA can be performed in high-risk individuals but is associated with significant cardiac risk. It remains difficult to risk stratify patients using preoperative stress testing. Echo evaluation may help to identify patients who may be more likely to develop ventricular arrhythmias in the postoperative period and thus warrant closer monitoring. Postoperative troponin monitoring of all patients undergoing repair of CAA is warranted given the overall risk of MI
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