Platelets retain inducible alpha granule secretion by P‐selectin expression but exhibit mechanical dysfunction during trauma‐induced coagulopathy

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149281/1/jth14414.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/149281/2/jth14414_am.pd

A RT-qPCR system using a degenerate probe for specific identification and differentiation of SARS-CoV-2 Omicron (B.1.1.529) variants of concern

Fast surveillance strategies are needed to control the spread of new emerging SARS-CoV-2 variants and gain time for evaluation of their pathogenic potential. This was essential for the Omicron variant (B.1.1.529) that replaced the Delta variant (B.1.617.2) and is currently the dominant SARS-CoV-2 variant circulating worldwide. RT-qPCR strategies complement whole genome sequencing, especially in resource lean countries, but mutations in the targeting primer and probe sequences of new emerging variants can lead to a failure of the existing RT-qPCRs. Here, we introduced an RT-qPCR platform for detecting the Delta- and the Omicron variant simultaneously using a degenerate probe targeting the key ΔH69/V70 mutation in the spike protein. By inclusion of the L452R mutation into the RT-qPCR platform, we could detect not only the Delta and the Omicron variants, but also the Omicron sub-lineages BA.1, BA.2 and BA.4/BA.5. The RT-qPCR platform was validated in small- and large-scale. It can easily be incorporated for continued monitoring of Omicron sub-lineages, and offers a fast adaption strategy of existing RT-qPCRs to detect new emerging SARS-CoV-2 variants using degenerate probes.</p

Soziale Unterstuetzung, Stresserleben und Zufriedenheit beim Auslandsaufenthalt (Social Support, Job Stress and Psychological Wellbeing on Foreign Assignments)

Diese Studie mit 127 Entsandten betrachtet im Unterschied zu bisherigen Studien soziale Unterstuetzung im Kontext von Auslandsentsendungen differenzierter und untersucht deren Auswirkung auf die Zufriedenheit und Stresserleben. Es wurde nach Quelle – Familie, Freunde, Arbeitskollegen und Vorgesetzte – und nach Art der sozialen Unterstuetzung (instrumentell oder sozioemotional) differenziert. Die Unterstuetzung durch den Vorgesetzten und instrumentelle Unterstuetzung mindern das Stresserleben im Ausland. Lebenszufriedenheit kann durch die Unterstuetzung des Ehepartners und durch Freunde vorhergesagt werden, die Arbeitszufriedenheit hingegen durch die Unterstuetzung des Vorgesetzten und durch instrumentelle Unterstuetzung. (Contrary to previous investigations, this study of 127 expatriates takes a more differentiated approach to examining the influence of social support on satisfaction and stress. It differentiates between source of support – influence of family, friends, co-workers and supervisors – and type of support (instrumental vs. socio-emotional). Support from supervisors and instrumental support reduces stress. Well-being is dependent on support from spouse and friends, whereas job satisfaction is dependent on support from supervisors and instrumental support.)foreign assignment, social support, job satisfaction, life satisfaction, job stress

What is not beautiful should match: how attractiveness similarity affects consumer responses to advertising

This study introduces the concept of attractiveness similarity, empirically examines its main effect and whether it moderates the effect of endorser attractiveness on consumer responses to advertising. The results show a positive main effect of attractiveness similarity over and above the mere effect of endorser attractiveness. In addition, a consistent moderating effect of attractiveness similarity on the effect of endorser attractiveness emerges: attractiveness similarity buffers against the less positive effects of lower levels of endorser attractiveness (i.e., it compensates for lower levels of endorser attractiveness). Overall, these findings reveal attractiveness similarity as a new variable in endorser advertising, which has important managerial implications. Advertising campaigns employing averagely attractive endorsers should pay special attention to attractiveness similarity

Wertkonflikte und Sozialisierungseffekte Ergebnisse einer Laengsschnittstudie ueber den Berufseintritt von Akademikern

UuStB Koeln(38)-880106140 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Selektion und Sozialisation potentieller Fuehrungskraefte im Zeichen gesellschaftlichen Wertwandels Ergebnisse einer Laengsschnittstudie des Uebergangs vom Bildungs- ins Beschaeftigungssystem

UuStB Koeln(38)-880106141 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Determination of protein haptenation by chemical sensitisers within the complexity of the human skin proteome: protein-sensitiser haptenation of complex cellular lysates

Skin sensitisation associated with the development of allergic contact dermatitis (ACD) occurs via a number of specific key events at the cellular level. The molecular initiating event (MIE), the first in the sequence of these events, occurs after exposure of the skin to an electrophilic chemical, causing the irreversible haptenation of proteins within skin. Characterisation of this MIE is a key step in elucidating the skin sensitisation adverse outcome pathway and is essential to providing parameters for mathematical models to predict the capacity of a chemical to cause sensitisation. As a first step to addressing this challenge, we have exposed complex protein lysates from a keratinocyte cell line and human skin tissue with a range of well characterised sensitisers, including dinitrochlorobenzene (DNCB), 5-chloro-2-methylisothiazol-3-one (MCI), cinnamaldehyde (CA) and the non (or weak) sensitiser 6-methyl coumarin (6-MC). Using a novel stable isotope labelling approach combined with ion mobility assisted data independent mass spectrometry (HDMSE), we have characterised the haptenome for these sensitisers. Although a significant proportion of highly abundant proteins were haptenated, we also observed the haptenation of low abundant proteins by all three of the chemical sensitisers tested, indicating that within a complex protein background, protein abundance is not the sole determinant driving haptenation, highlighting a relationship to tertiary protein structure and the amino acid specificity of these chemical sensitisers and sensitiser potency. <br/