2 research outputs found
Efeito do retardamento da colheita, na qualidade e na vida útil do melão Orange Flesh Effect of the harvest delay on the quality and post-harvest shelf-life of Orange Flesh melons
O efeito do retardamento da colheita na qualidade e na vida útil dos frutos do meloeiro foi avaliado. Os frutos, tipo Honey Dew Orange Flesh, foram colhidos no dia adotado pelos produtores como maturidade comercial, um, dois, três e quatro dias após e, foram armazenados a 7 ± 1ºC e UR de 90 ± 5%. O experimento foi conduzido obedecendo delineamento inteiramente casualizado em esquema fatorial 5 x 5, com cinco repetições, cinco tempos de retardamento (0; 1; 2; 3 e 4 dias após o adotado para a colheita) e cinco tempos de armazenamento (0; 7; 14; 21 e 28 dias) após a colheita. Foram avaliado a aparência externa e interna, firmeza de polpa, conteúdo de sólidos solúveis e incidência de rachadura no pedúnculo. Foi observada perda gradativa de firmeza da polpa para todos os retardamentos durante o armazenamento. Ao final do experimento os frutos ainda apresentavam aparência própria à comercialização. O conteúdo de sólidos solúveis ficou entre 9 e 12% e a incidência de rachaduras foi menor para os frutos colhidos aos 59; 60 e 61 dias após o plantio.<br>The effect of harvesting delay on quality and postharvest shelf-life of Honey Dew Orange Flesh melons was examined. Fruits were harvested at the stage of commercial maturity and, one, two, three and four days after this period. Fruits were kept at 7 ± 1ºC and 90 ± 5% relative humidity. A 5 x 5 factorial scheme in a completely randomized design with five replications was used, with five harvesting dates (0; 1; 2; 3 and 4 days after the stage of commercial harvest) and five storage periods (0; 7; 14; 21 and 28 days). During this period we evaluated the external and internal appearances, flesh firmness, soluble solid content and crack incidence of the peduncle. There occurred reduction of the flesh firmness for all harvest delaying dates. Fruits presented appropriate marketing appearance until the end of the experiment. The soluble solids content varied from 9 to 12%, and the cracking incidence was smaller on fruits harvested at 59; 60 and 61 days after planting date
New insights into the biology and origin of mature aggressive B-cell lymphomas by combined epigenomic, genomic, and transcriptional profiling
Lymphomas are assumed to originate at
different stages of lymphocyte development
through chromosomal aberrations.
Thus, different lymphomas resemble lymphocytes
at distinct differentiation stages
and show characteristic morphologic, genetic,
and transcriptional features. Here,
we have performed a microarray-based
DNA methylation profiling of 83 mature
aggressive B-cell non-Hodgkin lymphomas
(maB-NHLs) characterized for their
morphologic, genetic, and transcriptional
features, including molecular Burkitt lymphomas
and diffuse large B-cell lymphomas.
Hierarchic clustering indicated that
methylation patterns in maB-NHLs were
not strictly associated with morphologic,
genetic, or transcriptional features. By
supervised analyses, we identified
56 genes de novo methylated in all lymphoma
subtypes studied and 22 methylated
in a lymphoma subtype–specific
manner. Remarkably, the group of genes
de novo methylated in all lymphoma subtypes
was significantly enriched for polycomb
targets in embryonic stem cells. De
novo methylated genes in all maB-NHLs
studied were expressed at low levels in
lymphomas and normal hematopoietic tissues
but not in nonhematopoietic tissues.
These findings, especially the enrichment
for polycomb targets in stem
cells, indicate that maB-NHLs with different
morphologic, genetic, and transcriptional
background share a similar stem
cell–like epigenetic pattern. This suggests
that maB-NHLs originate from cells
with stem cell features or that stemness
was acquired during lymphomagenesis
by epigenetic remodeling