Life Cycle Assessment and Techno-Economic Analysis for Anaerobic Digestion as Cow Manure Management System

Clean electricity is generated by the anaerobic digestion of biomass waste. The environmental impacts of various biomass waste feedstocks vary, while co-digestion has been reported to improve anaerobic digestion performance. A consequential life-cycle assessment (LCA) and techno-economic analysis (TEA) are carried out for cow manure waste management for a cow farm. Three scenarios are considered in this study: (S1) mono-digestion of cow manure, (S2) co-digestion of cow manure and maize silage, and (S3) co-digestion of cow manure with cow feed waste, sewage sludge, and returned dairy products. The LCA aims to quantify the environmental impact of each MWh of electricity generated, assuming the plant is located in Malaysia, using OpenLCA software. The TEA economic parameters are quantified and compared between the three scenarios. Net present value (NPV), Internal Return Rate (IRR), and Return of Investment (ROI) are examined. Among the three scenarios, S2 with maize cultivation has a higher environmental impact due to its higher energy requirements. With the integration of closed digestate storage and renewable energy-powered electricity, S3 has the best environmental performance in global warming, eutrophication and acidification. S3 is found to be most economically viable, with MYR 1.28 million NPV, 14% IRR, and 15% ROI, and a Payback Period of 6.56 years with an OPEX of MYR 3491.82/MWh

High efficiency cell-specific targeting of cytokine activity

International audienceSystemic toxicity currently prevents exploiting the huge potential of many cytokines for medical applications. Here we present a novel strategy to engineer immunocytokines with very high targeting efficacies. The method lies in the use of mutants of toxic cytokines that markedly reduce their receptor-binding affinities, and that are thus rendered essentially inactive. Upon fusion to nanobodies specifically binding to marker proteins, activity of these cytokines is selectively restored for cell populations expressing this marker. This ‘activity-bytargeting’ concept was validated for type I interferons and leptin. In the case of interferon, activity can be directed to target cells in vitro and to selected cell populations in mice, with up to 1,000-fold increased specific activity. This targeting strategy holds promise to revitalize the clinical potential of many cytokines