Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β stimulated SK-N-SH cells

Purpose: Pomegranate fruit, Punica granatum L. (Punicaceae) and its constituents have been shown to inhibit inflammation. In this study we aimed to assess the effects of freeze-dried pomegranate (PWE) on PGE2 production in IL-1β stimulated SK-N-SH cells. Methods: An enzyme immuno assay (EIA) was used to measure prostaglandin E2 (PGE2) production from supernatants of IL-1β stimulated SK-N-SH cells. Expression of COX-2, phospho-IκB and phospho-IKK proteins were evaluated, while NF-κB reporter gene assay was carried out in TNFα-stimulated HEK293 cells to determine the effect of PWE on NF-κB transactivation. Levels of BACE-1 and Aβ in SK-N-SH cells stimulated with IL-1β were measured with an in cell ELISA. Results: PWE (25-200 µg/ml) dose dependently reduced COX-2 dependent PGE2 production in SK-N-SH cells stimulated with IL-1β. Phosphorylation of IκB and IKK were significantly (p<0.001) inhibited by PWE (50- 200 µg/ml). Our studies also show that PWE (50-200 µg/ml) significantly (p<0.01) inhibited NF-κB transactivation in TNFα-stimulated HEK293 cells. Furthermore PWE inhibited BACE-1 and Aβ expression in SK-N-SH cells treated with IL-1β. Conclusions: Taken together, our study demonstrates that pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders like Alzheimer’s disease

Explaining noncompliance in the Norwegian coastal cod fishery: an application of the multinomial logit

Establishing motive is central to the analysis of criminal behaviour. This article analyses the range of motives for noncompliant behaviour among coastal cod fishermen. A multinomial logit model is employed to analyse the reasons that underlie various motives. The four motives compared in this study are economic, technological and social and motives caused by bureaucracy and legitimacy problems. The economic motive is found to be most important for coastal fishermen. However, the cross-sectional data indicate that motives vary with fishing gear, vessel length and fishermen's age. The results indicate that identifying motives for noncompliant behaviour is not straightforward but is important for reducing the extent of infringements of the regulations. Fishermen who use gill net, seine and long line, for example, are more often motivated to noncompliance by technical problems and bureaucracy than hand-jig fishers. The study suggests that in order to prevent noncompliance behaviour, the authorities' management policy should be designed to address the problems encountered by different categories of fishers. For example, offences that are committed because of technical problems should be resolved by increasing the involvement of fishermen in the process of drawing up regulations, whereas offences motivated by economic factors should be reduced by increasing inspections.