5 research outputs found
ΠΡΠΎΠ±Π»Π΅ΠΌΠΈ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠΎΡΠΏΡΠΎΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π²ΡΡΡΠΈΠ·Π½ΡΠ½ΠΈΡ ΡΡΡΠ°Ρ ΠΎΠ²ΠΈΡ ΠΊΠΎΠΌΠΏΠ°Π½ΡΠΉ Π² ΡΠΌΠΎΠ²Π°Ρ Π³Π»ΠΎΠ±Π°Π»ΡΠ·Π°ΡΡΡ
A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiseru
Effect of aquaculture on world fish supplies
Global production of farmed fish and shellfish has more than doubled in the past 15 years. Many people believe that such growth relieves pressure on ocean fisheries, but the opposite is true for some types of aquaculture. Farming carnivorous species requires large inputs of wild fish for feed. Some aquaculture systems also reduce wild fish supplies through habitat modification, wild seedstock collection and other ecological impacts. On balance, global aquaculture production still adds to world fish supplies; however, if the growing aquaculture industry is to sustain its contribution to world fish supplies, it must reduce wild fish inputs in feed and adopt more ecologically sound management practices.We thank M. Williams, W. Falcon, V. Spruill, M. Drew, N. Wada, R. Kautsky, K. Jauncey, C. Tirado, R. Hoguet, R. Tatum and R. Mitchell for comments and assistance, and the David and Lucile Packard Foundation for funding