Removal Of Cyanobacteria Toxins From Drinking Water By Adsorption On Activated Carbon Fibers

Natural fibers from macadamia nut shell, dried coconut shell endocarp, unripe coconut mesocarp, sugarcane bagasse and pine wood residue were used to prepare activated carbon fibers (ACF) with potential application for removing microcystins. The ACF from pine wood and sugar cane bagasse were used to remove [D-Leucine1 MCYST-LR from water. After 10 minutes of contact time, more than 98% of toxin was removed by the ACF. The microcystin adsorption monolayer, qm, in the ACF recovered 200 and 161 μg.mg-1, with the Langmuir adsorption constant, KL, of 2.33 and 1.23 L.mg-1. Adsorption of [D-Leucine1]MCYST-LR in continuous process was studied for a fixed-bed ACF prepared from coconut shell and sugar cane bagasse and for two commercial activated carbon samples from treatment water plants of two Brazilian hemodialysis centers. 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A study of the MAYV replication cycle: correlation between the kinetics of viral multiplication and viral morphogenesis

Mayaro virus (MAYV) is mainly found in Central and South America and causes a febrile illness followed by debilitating arthritis and arthralgia similar to chikungunya virus (CHIKV). Infection leads to long-term sequelae with a direct impact on the patient's productive capacity, resulting in economic losses. Mayaro fever is a neglected disease due to the limited epidemiological data. In Brazil, it is considered a potential public health risk with the number of cases increasing every year. Most of our knowledge about MAYV biology is inferred from data obtained from other alphaviruses as well as more recent studies on MAYV. Here, we analyzed the kinetics of viral replication through standard growth curves, quantification of intracellular and extracellular particles, and RNA quantification. We compared transmission electron microscopy data during different stages of infection. This approach allowed us to establish a chronological order of events during MAYV replication and its respective timepoints including cell entry through clathrin-mediated endocytosis occurring at 15-30 min, genome replication at 2-3 h, morphogenesis at 4 hpi, and release at 4-6 hpi. We also present evidence of uncharacterized events such as ribosome reorganization as well as clusters of early viral precursors and release through exocytosis in giant forms. Our work sheds new and specific light on the MAYV replication cycle and may contribute to future studies on the field