ИММУНОФЕНОТИПИЧЕСКАЯ ХАРАКТЕРИСТИКА ДЕНДРИТНЫХ КЛЕТОК ПРИ БРОНХИАЛЬНОЙ АСТМЕ В УСЛОВИЯХ ВОЗДЕЙСТВИЯ ЭКСТРАКТОМ OPISTHORCHIS FELINEUS IN VITRO

This work shows comparative characteristics of immunogenic properties of the extract of Opisthorchis felineus in different severity of asthma using antigen presenting dendritic cells in vitro. New data on the effect of Opisthorchis felineus on the expression of surface markers of dendritic cells (CD209, HLA-DR, CD83, CD86) were received. Pronounced expression of CD209, CD86 and HLA-DR on the surface of dendritic cells in mild and severe asthma compared with healthy individuals was shown. In the stimulation of dendritic cells with extract Opisthorchis felineus in vitro weakening of CD86 expression in mild and in severe asthma was found. CD86 molecule may be a regulatory factor in the co-stimulation of dendritic cells which allows us to consider it as a potential pharmacological target for the treatment of allergic diseases. Дана сравнительная характеристика иммуногенных свойств экстракта Opisthorchis felineus при бронхиальной астме различной степени тяжести с использованием антигенпрезентирующих дендритных клеток in vitro. Получены новые данные о влиянии Opisthorchis felineus на экспрессию поверхностных маркеров дендритных клеток (CD209, HLA-DR, CD83, CD86). Выявлена более выраженная экспрессия молекул CD209, CD86 и HLA-DR на поверхности дендритных клеток при легкой и тяжелой астме по сравнению с показателями здоровых лиц. В условиях стимуляции дендритных клеток экстрактом Opisthorchis felineus in vitro установлено ослабление экспрессии CD86 как при легкой, так и при тяжелой астме. Молекула CD86 может быть регуляторным фактором в костимуляции дендритных клеток, что позволяет рассматривать ее в качестве возможной фармакологической мишени для терапии аллергических болезней

Legal status, role and features of electronic document management

© 2020, Universidad del Zulia. All rights reserved. The study analyses the conditions and the degree of development of approaches to the legal regulation of electronic document management and the possibility of introducing this management in various areas at the international level.The study concluded that for the full implementation of electronic document management at the international level, it is necessary to create a trust service, which is an intermediary link with the powers and rights to verify the authenticity of electronic signatures and the legitimacy of digital documents. This will fully allow the use of the capabilities of telecommunication systems and electronic document management

Analysis of Monitoring Data on the Criteria of Technogenic Safety in Project Activities

Monitoring and control over the criteria of technogenic safety is carried out on the basis of a man-machine system in the design of technical systems with the participation of a group of experts in various fields of knowledge. Teamwork is carried out on the basis of multi-agent technologies in a distributed information environment for processing and transmitting information. The aim of the study is to identify a generalized indicator (a criterion of technological safety) - the degrees of risk used to take into account the possibility of the emergence of technological situations. © 2019 IEEE

Chimerism and multiple numerical chromosome imbalances in a spontaneously aborted fetus

We report on a case of chimerism and multiple abnormalities of chromosomes 21, X and Y in spontaneous abortion specimen. To the best our knowledge the present case is the first documented chimera in a spontaneously aborted fetus. The application of interphase fluorescence in situ hybridization (FISH) using chromosome enumeration and site-specific DNA probes showed trisomy X in 92 nuclei (23 %), tetrasomy X in 100 nuclei (25 %), pentasomy of chromosome X in 40 nuclei (10 %), XXY in 36 nuclei (9 %), XXXXXXYY in 12 nuclei (3 %), XXXXXYYYYY in 8 nuclei (2 %), trisomy 21 and female chromosome complement in 40 nuclei (10 %), normal female chromosome complement in 72 nuclei (18 %) out of 400 nuclei scored. Our experience indicates that the frequency of chimerism coupled with multiple chromosome abnormalities should be no less than 1 : 400 among spontaneous abortions. The difficulties of chimerism identification in fetal tissues are discussed

Application of machine learning algorithms in morphopathology and in assisted reproductive technologies

Machine learning models are used everywhere to analyze images, signals, and videos. At first glance, this is a well-designed process that involves the stages of data collection, mark-up, and training a model, and, as a result, its application in a particular field (recognition of vehicle plate numbers, smartphone faces, etc.). However, everything is much more complicated in the field of medicine: the use of artificial intelligence models is a serious challenge. Machine learning methods are becoming more and more used in morphological sciences and biomedical studies. The introduction of artificial intelligence for image analysis can lower the burden on an operator (a pathologist, a histologist), eliminate the factor of subjective assessment, and reduce the likelihood of an error. This review provides a brief excursion into the history of machine learning methods, considers the examples of their use in two areas where they are most widespread: morphopathology and assisted reproductive technologies, and also indicates the limitations and difficulties that developers face when training neural networks. Conclusion: The authors also propose solutions to overcome the difficulties associated with the collection and joint marking of data, and model training: creation of a high-quality infrastructure, attraction of highly qualified specialists who mark data, an advanced scientific approach to artificial intelligence technologies; cloud platforms are offered to be used as a basis for the scalable storage and analysis of biomedical data. © 2021, Bionika Media Ltd.. All rights reserved

ПРОИЗВОДНЫЕ АДАМАНТАНА, СПОСОБНЫЕ ИНГИБИРОВАТЬ РЕПРОДУКЦИЮ РЕЗИСТЕНТНОГО К РИМАНТАДИНУ ШТАММА ВИРУСА ГРИППА A(H1N1)PDM09 (INFLUENZA A VIRUS, ALPHAINFLUENZAVIRUS, ORTHOMYXOVIRIDAE)

Introduction. Adamantanthane-type drugs such as rimantadine and amantadine have long been used to treat diseases caused by influenza A virus. However, as a result of the mutations, influenza viruses have become resistant to aminoadamantans. The target for these drugs was the protein channel M2. Influenza A virus M2 viroporin in the protein shell forms fairly specific ion channels with a diameter of about 11 Å, specializing in transporting protons inside the viral particle (virion). Restoration of the antiviral properties of adamantanthane-type drugs consists in the selection of advanced functional groups bound by the carbocycle to find new sites of binding to the protein target M2. The purpose of the study is to identify the antiviral properties of new adamantanum derivatives to the pandemic strain of influenza A virus in vitro. Material and methods. Compounds of aminoadamantans with amino acids and other organic molecules were obtained by classical peptide synthesis methods. The structure of the compound was tested by means of physical and chemical methods. Antiviral properties of synthetic compounds were studied in vitro on monolayer MDCK cells infected with pandemic strain of influenza A/California/07/2009 virus in two schemes of administration of investigated compounds and virus. Results. The reference strain of the influenza virus A/California/07/2009(H1N1) was sensitive to the compounds under test in varying degrees. The antiviral activity of the compounds was expressed in a 50% inhibitory concentration (IC50) ranging from 0.5 to 2.5 mkM, which is generally a good indicator for the Rimantadine/Amantadine resistant strain. Discussion. The values of the IC50 for compounds introduced two hours before contact with the virus were slightly higher than those for single-moment introduction of the substance and virus. The effect of increasing the inhibitory concentration in the prophylactic scheme of compounds was valid for all compounds of the experiment. Conclusion. The presented synthetic compounds are active against the variant of influenza A virus resistant to Rimantadine and Amantadine preparations. The obtained compounds can be used as model structures for creation of a new drug of direct action against advanced strains of influenza A virus.Введение. Соединения адамантанового ряда, такие как римантадин и амантадин, долгое время применяли для лечения заболеваний, вызванных вирусом гриппа А. Однако в результате возникших мутаций вирусы гриппа приобрели резистентность к аминоадамантанам. Мишенью для этих препаратов служил белковый канал М2. Виропорин М2 в белковой оболочке вируса гриппа А образует достаточно специфичные ионные каналы диаметром около 11 Å, специализирующиеся на транспорте ионов водорода внутрь вирусной частицы (вириона). Восстановление противовирусных свойств препаратов адамантанового ряда заключается в подборе дополнительных функциональных групп, связанных карбоциклом, для поиска новых сайтов связывания с белком мишенью М2. Цель исследования - выявление противовирусных свойств адамантановых производных в отношении пандемического штамма вируса гриппа А in vitro. Материал и методы. Соединения аминоадамантанов с аминокислотами и другими органическими молекулами были получены методами классического пептидного синтеза. Структура соединения подтверждена современными физико-химическими методами. Противовирусные свойства синтетических соединений были изучены in vitro на монослое клеток MDCK, инфицированных пандемическим штаммом вируса гриппа А/California/07/2009 в двух схемах введения исследуемых соединений и вируса. Результаты. Эталонный штамм вируса гриппа A/California/07/2009(H1N1) был в разной мере чувствителен к тестируемым соединениям. Противовирусную активность соединений выражали в виде 50% ингибирующей дозы (ИД50), которая составила от 0,5 до 2,5 мкМ, что в целом неплохой показатель в отношении штамма, резистентного к римантадину/амантадину. Обсуждение. ИД50 для соединений, вносимых за 2 ч до контакта с вирусом, была несколько выше, чем при одномоментном внесении вещества и вируса. Эффект увеличения ингибирующей концентрации в профилактической схеме внесения соединений был справедлив для всех соединений эксперимента. Заключение. Представленные синтетические соединения активны в отношении варианта вируса гриппа А, резистентного к римантадину и амантадину. Полученные соединения могут быть использованы в качестве модельных структур для создания нового препарата прямого действия против современных штаммов вируса гриппа А