43 research outputs found
Cooperativity Scale: A StructureâMechanism Correlation in the Self-Assembly of Benzene-1,3,5-tricarboxamides
ConspectusThe self-assembly of small and well-defined
molecules using noncovalent interactions to generate various nano-
and microarchitectures has been extensively studied. Among various
architectures, one-dimensional (1-D) nano-objects have garnered significant
attention. It has become increasingly evident that a cooperative or
nucleationâelongation mechanism of polymerization leads to
highly ordered 1-D supramolecular polymers, analogous to shape-persistent
biopolymers such as actin. With this in mind, achieving cooperativity
in self-assembled structures has been actively pursued with significant
success. Only recently, researchers are focusing on the origin of
the mechanism at the molecular level in different synthetic systems.
Taking a step further, a thorough quantitative structureâmechanism
correlation is crucial to control the size, shape, and functions of
supramolecular polymers, and this is currently lacking in the literature.Among a plethora of molecules, benzene-1,3,5-tricarboxamides (BTAs)
provide a unique combination of important noncovalent interactions such as hydrogen bonding, Ď-stacking, and hydrophobic interactions,
for self-assembly and synthetic ease. Due to the latter, a diverse
range of BTA derivatives with all possible structural mutations have
been synthesized and studied during the past decade, mainly from our
group. With such a large body of experimental results on BTA self-assembly,
it is time to embark on a structureâmechanism correlation in
this family of molecules, and a first step toward this will form the
main focus of this Account. The origin of the cooperative mechanism
of self-assembly in BTAs has been ascribed to 3-fold intermolecular
hydrogen bonding (HB) between monomers based on density-functional
theory (DFT) calculations. The intermolecular hydrogen-bonding interaction
forms the central premise of this work, in which we evaluate the effect
of different moieties such as alkyl chains, and amino
acids, attached to the core amides on the strength of intermolecular
HB, which consequently governs the extent of cooperativity (quantified
by the cooperativity factor, Ď). In addition to this, we evaluate
the effect of amide connectivity (C- vs N-centered), the role of solvents,
amides vs thioamides, and finally the influence of the benzene vs
cyclohexane core on the Ď. Remarkably, every subtle structural
change in the BTA monomer seems to affect the cooperativity factor
in a systematic and rationalizable way.The take home message
will be that the cooperativity factor (Ď) in the BTA family
forms a continuous spectrum from 1 (isodesmic) to <10<sup>â6</sup> (highly cooperative) and it can be tuned based on the appropriate
modification of the BTA monomer. We anticipate that these correlations
drawn from the BTA series will be applicable to other systems in which
HB is the main driving force for cooperativity. Thus, the understanding
gained from such correlations on a prototypical self-assembling motif
such as BTA will aid in designing more complex systems with distinct
functions
Supramolecular Platform Stabilizing Growth Factors
High
concentrations of supplemented growth factors can cause oversaturation
and adverse effects in <i>in vitro</i> and <i>in vivo</i> studies, though these supraphysiological concentrations are often
required due to the low stability of growth factors. Here we demonstrate
the stabilization of TGF-β1 and BMP4 using supramolecular polymers.
Inspired by heparan sulfate, sulfonated peptides were presented on
a supramolecular polymer to allow for noncovalent binding to growth
factors in solution. After mixing with excipient molecules, both TGF-β1
and BMP4 were shown to have a prolonged half-life compared to the
growth factors free in solution. Moreover, high cellular response
was measured by a luciferase assay, indicating that TGF-β1 remained
highly active upon binding to the supramolecular assembly. The results
demonstrate that significant lower concentrations of growth factors
can be used when supramolecular polymers bearing growth factor binding
moieties are implemented. This approach can also be exploited in hydrogel
systems to control growth factor release
Pathway Selection in Peptide Amphiphile Assembly
The nature of supramolecular
structures could be strongly affected
by the pathways followed during their formation just as mechanisms
and final outcomes in chemical reactions vary with the conditions
selected. So far this is a largely unexplored area of supramolecular
chemistry. We demonstrate here how different preparation protocols
to self-assemble peptide amphiphiles in water can result in the formation
of different supramolecular morphologies, either long filaments containing
β-sheets or smaller aggregrates containing peptide segments
in random coil conformation. We found that the assembly rate into
β-sheets decreases in the presence of a destabilizing âgoodâ
solvent like hexafluoroisopropanol (HFIP) and is affected by transient
conditions in solution. Also the peptide amphiphile investigated spontaneously
nucleates the β-sheet-containing filaments at a critical fraction
of HFIP in water below 21%. Furthermore, β-sheet assemblies
have a high kinetic stability and, once formed, do not disassemble
rapidly. We foresee that insights into the characteristic dynamics
of a supramolecular system provide an efficient approach to select
the optimum assembly pathway necessary for function
Supramolecular Buffering by RingâChain Competition
Recently, we reported an organocatalytic
system in which buffering
of the molecular catalyst by supramolecular interactions results in
a robust system displaying concentration-independent catalytic activity.
Here, we demonstrate the design principles of the supramolecular buffering
by ringâchain competition using a combined experimental and
theoretical approach. Our analysis shows that supramolecular buffering
of a molecule is caused by its participation as a chain stopper in
supramolecular ringâchain equilibria, and we reveal here the
influence of various thermodynamic parameters. Model predictions based
on independently measured equilibrium constants corroborate experimental
data of several molecular systems in which buffering occurs via competition
between cyclization, growth of linear chains, and end-capping by the
chain-stopper. Our analysis reveals that the effective molarity is
the critical parameter in optimizing the broadness of the concentration
regime in which supramolecular ringâchain buffering occurs
as well as the maximum concentration of the buffered molecule. To
conclude, a side-by-side comparison of supramolecular ringâchain
buffering, pH buffering, and molecular titration is presented
Amplifying Chiroptical Properties of Conjugated Polymer Thin-Film Using an Achiral Additive
Chiral conjugated polymers bearing
enantiopure side chains offer
the possibility to harness the effect of chirality in organic electronic
devices. However, its use is hampered by the low degree of circular
polarization in absorption (<i>g</i><sub>abs</sub>) in most
of the conjugated polymer thin-films studied. Here we demonstrate
a versatile method to significantly increase the <i>g</i><sub>abs</sub> by using a few weight percentages of a commercially
available achiral long-chain alcohol as an additive. This additive
enhances the chiroptical properties in both absorption and emission
by ca. 5â10 times in the thin-films. We envisage that the alcohol
additive acts as a plasticizer which enhances the long-range chiral
liquid crystalline ordering of the polymer chains, thereby amplifying
the chiroptical properties in the thin-film. The application of this
methodology to various conjugated polymers has been demonstrated
Efficient Routes to A<sub>3</sub>BâType <i>meso</i>-(4-Carboxyphenyl) Porphyrin Derivatives
A<sub>3</sub>B-type <i>meso</i>-(4-carboxyphenyl) porphyrins
were prepared either by stepwise coupling of aniline substituents
to <i>meso</i>-tetrakisÂ(4-carboxyphenyl) porphyrin (TCPP)
or by utilizing its partially protected trimethyl ester derivative.
We demonstrate the high utility of this building block, which can
be synthesized in very good yields by microwave-assisted Me<sub>3</sub>SnOH hydrolysis
From Molecular Structure to Macromolecular Organization: Keys to Design Supramolecular Biomaterials
In
the past decade, significant progress has been made in the field
of biomaterials, for potential applications in tissue engineering
or drug delivery. We have recently developed a new class of thermoplastic
elastomers, based on ureidopyrimidinone (UPy) quadruple hydrogen bonding
motifs. These supramolecular polymers form nanofiber-like aggregates
initially <i>via</i> the dimerization of the UPy units followed
by lateral urea-hydrogen bonding. Combined kinetic and thermodynamic
studies unravel the pathway complexity in the formation of these polymorphic
nanofibers and the subtlety of the polymerâs design, while
these morphologies are so critically important when these materials
are used in combination with cells. We also show that the cell behavior
directly depends on the length and shape of the nanofibers, illustrating
the key importance of macromolecular and supramolecular organization
of biomaterials. This study leads to new design rules that determine
what factors are decisive for a polymer to be a good candidate as
biomaterial
Self-Assembly of Hydrogen-Bonding Gradient Copolymers: Sequence Control via Tandem Living Radical Polymerization with Transesterification
Chiral
1,3,5-tricarboxamide (BTA)-functionalized copolymers with
gradient, bidirectional gradient, and random sequence distributions
were synthesized via tandem living radical polymerization (LRP) with
in situ monomer transesterification to investigate the effects of
the BTA sequence on self-folding/aggregation properties in organic
media. Here, 2-ethylhexyl methacrylate (EHMA) as a starting monomer
was polymerized with a ruthenium catalytic system in the presence
of a chiral BTA-bearing alcohol (BTA-OH) and TiÂ(O<i>i</i>-Pr)<sub>4</sub>. By tuning the concentration and time of addition
of the Ti catalyst, the transesterification rate of EHMA into a chiral
BTA-functionalized methacrylate (BTAMA) was synchronized with LRP
to produce EHMA/BTAMA gradient or bidirectional gradient copolymers.
In contrast, faster transesterification than LRP gave the corresponding
random copolymer. Circular dichroism spectroscopy and dynamic light
scattering performed on solutions of all BTA-functionalized copolymers
indicated that the chiral BTA pendants self-assemble helically via
hydrogen-bonding interaction in 1,2-dichloroethane, methylÂcyclohexane
(MCH), and their mixtures to form single-chain or multichain polymeric
nanoparticles. The temperature-dependent self-assembly behavior of
the BTA pendants was virtually independent of the sequence distribution,
whereas the size of the resultant nanoparticles depended on the sequence
as follows: random < gradient < bidirectional gradient in MCH
Supramolecular Loop Stitches of Discrete Block Molecules on Graphite: Tunable Hydrophobicity by Naphthalenediimide End-Capped Oligodimethylsiloxane
The
noncovalent functionalization of surfaces has gained widespread
interest in the scientific community, and it is progressively becoming
an extremely productive research field offering brand new directions
for both supramolecular and materials chemistry. As the end-groups
often play a dominant role in the surface properties obtained, creating
loops with end-groups only at the surface will lead to unexpected
architectures and hence properties. Here we report the self-assembly
of discrete block moleculesî¸structures in-between block copolymers
and liquid crystalsî¸featuring oligodimethylsiloxanes (ODMS)
end-capped with naphthalenediimides (NDIs) at the 1-phenyloctane/highly
oriented pyrolytic graphite (1-PO/HOPG) interface. These structures
produce unprecedented vertically nanophase-separated monolayers featuring
NDI moieties that regularly arrange on the HOPG surface, while the
highly dynamic ODMS segments form loops above them. Such arrangement
is preserved upon drying and generates hydrophobic HOPG substrates
in which the ODMS block length tunes the hydrophobicity. Thus, the
exact structural fidelity of the discrete macromolecules allows for
the correlation of nanoscopic organization with macroscopic properties
of the self-assembled materials. We present a general strategy for
tunable hydrophobic coatings on graphite based on molecularly combining
crystalline aromatic moieties and immiscible oligodimethylsiloxanes
Sticky Supramolecular Grafts Stretch Single Polymer Chains
The
folding of single polymeric chains into single chain polymeric nanoparticles
(SCPNs) is a unique strategy to prepare ordered structures at the
nanoscopic level. Structure forming elements are attached to a polymer
chain designed to fold it into a well-defined object, the SCPN. The
self-assembly of these units has been investigated in great detail.
However, little is known about the impact of the resulting secondary
structure on the conformation of the polymer chain. Here we employ
a combination of scattering methods and spectroscopy to study how
pendant chiral benzene-1,3,5-tricarboxamides (BTAs) fold oligoÂ(ethylene
glycol) methyl ether methacrylate-based polymers into SCPNs. Circular
dichroism spectroscopy shows that the extent of BTA self-assembly
on the polymer chain in water can be fine-tuned by means of temperature
and cosolvent addition (isopropanol). Small-angle neutron scattering
experiments demonstrate that single polymer chains have an asymmetric
shape with a constant cross section, <i>R</i><sub>cs</sub>, and variable length, <i>L</i>, with <i>L</i> > <i>R</i><sub>cs</sub>. The polymer chain extends
and shortens in response to variations in temperature and solvent
composition, which also influence the self-assembly of the BTA units.
The SCPNs stretch upon association and shrink upon disassociation
of the grafted supramolecular moieties