‘In vivo’ optical approaches to angiogenesis imaging

In recent years, molecular imaging gained significant importance in biomedical research. Optical imaging developed into a modality which enables the visualization and quantification of all kinds of cellular processes and cancerous cell growth in small animals. Novel gene reporter mice and cell lines and the development of targeted and cleavable fluorescent “smart” probes form a powerful imaging toolbox. The development of systems collecting tomographic bioluminescence and fluorescence data enabled even more spatial accuracy and more quantitative measurements. Here we describe various bioluminescent and fluorescent gene reporter models and probes that can be used to specifically image and quantify neovascularization or the angiogenic process itself

Полихлорированные дибензо-п-диоксины и фураны: методы анализа, распределение по территории Москвы и применение к ним методов биотестирования

The review presents data on chemical-analytical methods of PCDD, PCDF determination and biotesting methods used for toxicological assessment of pollutants. Distribution of PCDDs, PCDFs on the territory of Moscow is analyzed. Analysis of publications of Russian and foreign authors showed that the currently existing methods of biotesting are in fact untested for dioxins. In order to quickly establish the toxicological effect of persistent organic pollutants in soil, it is suggested to use biotesting methods, with inclusion of test organisms representing the main trophic levels of ecosystems: producers, consumers, and decomposers.Приведены данные по химико-аналитическим методам определения ПХДД, ПХДФ и методам биотестирования, применяемым для токсикологической оценки загрязняющих веществ. Проанализировано распределение ПХДД, ПХДФ по территории Москвы. Анализ публикаций российских и зарубежных авторов показал, что существующие на настоящий момент методы биотестирования фактически не отработаны на диоксинах. Для быстрого установления токсикологического эффекта стойких органических загрязнителей в почве предлагается использование методов биотестирования, с включением тест-организмов, представляющих основные трофические уровни экосистем: продуцентов, консументов, редуцентов

REALITIES AND PROSPECTS FOR PHARMACOLOGICAL CORRECTION OF «ADMA-ENOS"-ASSOCIATED WAYS IN PREECLAMPSIA

Methylated analogs of L-arginine - asymmetric dimetilarginin (ADMA) and monometilarginin (L-NMMA) - are endogenous inhibitors of endothelial NO-synthase (eNOS). ADMA level in maternal plasma is increased in women with preeclampsia. The high level of ADMA is one of the predictors of preeclampsia. L-arginine increases the activity of eNOS and production of nitric oxide in the ADMA-similar model of L-NAME-induced endothelial dysfunction. ENOS activator (resveratrol), antioxidants, potentiated polyclonal antibodies to eNOS and others agents have been effective in this model. Studies of endotelioprotective activity in other «ADMA-eNOS-associated" experimental models of metabolic syndrome and homocysteine-induced, gipoestrogen-induced sepsis-induced endothelial dysfunction and endothelial dysfunction in postvaccinal vasculitis have been developed