The local crystallization in nanoscale diamond-like carbon films during annealing

The local crystallization during annealing at 600° C in nanoscale diamond-like carbon coatings films grown by pulsed vacuum-arc deposition method was observed using modern techniques of high-resolution transmission electron microscopy. The crystallites formed by annealing have a face-centred cubic crystal structure and grow in the direction [011] as a normal to the film surface. The number and size of the crystallites depend on the initial values of the intrinsic stresses before annealing, which in turn depend on the conditions of film growth. The sizes of crystallites are 10 nm for films with initial compressive stresses of 3 GPa and 17 nm for films with initial compres- sive stresses of 12 GPa. Areas of local crystallization arising during annealing have a structure dif- ferent from the graphit

Information technology in the organization of interaction between teacher-student

The article considers the organization of modern, comfortable learning environment through interaction teacher-student with the help of information technology. The results of the Express survey of students and teachers actively using it in educationВ статье рассматривается организация современной, комфортной образовательной среды через взаимодействие преподаватель-студент с помощью информационных технологий. Приведены результаты экспресс-опроса студентов и преподавателей, активно использующих ИТ в образовани

Soil ph Control in the Mobile Corrosion Monitoring

Corrosion monitoring methods and pH measuring devices are studied. Considering the heterogeneity of the soil pH, depending on depth and sampling points, it is advisable to use solid-state potentiometric sensors that are capable of pH measuring with an error not exceeding 0.2 pH units. In the process of the pipeline installation necessary to control the soil pH value. While it is impossible to predict what kind of horizon will be in contact with the pipe material at different points. This problem requires future development of technique for the correct pH measuring for the purpose of mobile pipeline corrosion monitoring. When using cathodic protection has been required find the balance between the parameters which prevent the corrosion reactions and also take into account the hydrogen absorption on surface of the pipes material

TECHNOLOGICAL POSSIBILITIES OF PIPELINE-FREE GASIFICATION OF SETTLEMENTS USING LNG

The analysis of technological possibilities of pipeline-free gasification of settlements with natural gas using the resource base of low-tonnage LNG production at GDS and regasification complexes is performed.Выполнен анализ технологических возможностей беструбопроводной газификации поселений природным газом с использованием ресурсной базы малотоннажного производства сжиженного природного газа (СПГ) на газораспределительных станциях (ГРС) и регазификационных комплексах

Three-step procedure for preparation of pure Bacillus altitudinis ribonuclease

© 2015 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.Ribonucleases are considered as promising tools for anticancer treatment due to their selective cytotoxicity against tumor cells. We investigated a new RNase from Bacillus altitudinis termed BALNASE (B. altitudinisRNase). Balnase is a close homolog of the well-known cytotoxic binase, differing by only one amino acid residue: nonpolar hydrophobic alanine at position 106 in the balnase molecule is replaced by a polar uncharged threonine in binase. The most exciting question is how the physico-chemical properties and biological effects of RNase might be changed by A106T substitution. Here, we have developed a chromatography-based rapid and modern technique for the purification of this new RNase which allowed us to get a protein sample of high quality with specific activity of 1.2 × 106 units in preparative amounts, suitable for further investigation of its biological properties

Properties of carbon coatings obtained by pulsed high power methods of vacuum-arc and magnetron sputtering

Investigation results of the structure, mechanical, tribological and adhesion characteristics of carbon coatings obtained by two different methods: high-power impulse magnetron sputtering and pulsed cathodic arc deposition are presente

Bacteriophages of soil bacilli: A new multivalent phage of Bacillus altitudinis

© 2017, Allerton Press, Inc. Bacillus are soil saprophytes, facultative anaerobes developing in the temperature range of 28–37°С. 16S rRNA cataloging shows that these bacteria form a coherent class with broad variability of virulence. Bacillus phages can be extensively used for phagotyping bacteria in the process of soil, water, and food monitoring. Bacillus phages can also be used as vectors in horizontal gene transfer and potential therapeutic agents. Thus, description of the biological diversity of the Bacillus phages is useful for further development of tools used in molecular biology and biomedicine. In this work, the scheme for isolation of soil bacteriophages was unified, which allowed ten bacillus phages to be isolated from different types of soil. It was shown that the number of phages depended on the soil fertility, decreasing as the soil changed from black soil to chestnut soil to gray forest soil to uncontaminated urban soil to oil-contaminated urban soil. A new polyvalent DNA-containing bacteriophage SRT01hs of B. altitudinis (it is also able to infect B. subtilis, B. cereus, and B. pumilus, but not B. licheniformis and B. atrophaeus) was described in detail. It has a typical structure: a total length of 360 nm and an icosahedron-shaped head 100 nm in diameter. Several phages simultaneously attack a B. altitudinis cell by increasing the level of intracellular low-molecular RNA. Infection with the phage virtually eliminates the stationary growth phase of infected bacilli and leads to a permanent increase in the number of phages in cultural liquor, with the exception of the time period of high activity of the secreted ribonuclease

Polarization Dependence of Anomalous X-ray Scattering in Orbital Ordered Manganites

In order to determine types of the orbital ordering in manganites, we study theoretically the polarization dependence of the anomalous X-ray scattering which is caused by the anisotropy of the scattering factor. The general formulae of the scattering intensity in the experimental optical system is derived and the atomic scattering factor is calculated in the microscopic electronic model. By using the results, the X-ray scattering intensity in several types of the orbital ordering is numerically calculated as a function of azimuthal and analyzer angles.Comment: 9 pages, 7 figure

Ribonucleolytic activity of mycoplasmas

Mycoplasmas are incapable of de novo synthesis of nucleotides and must therefore secrete nucleases in order to replenish the pool of nucleic acid precursors. The nucleolytic activity of mycoplasmas is an important factor in their pathogenicity. Bacterial ribonucleases (RNases) may produce a broad spectrum of biological effects, including antiviral and antitumor activity. Mycoplasma RNases are therefore of interest. In the present work, the capacity of Acholeplasma laidlawii and Mycoplasma hominis for RNase synthesis and secretion was studied. During the stationary growth phase, these organisms were found to synthesize Mg2+-dependent RNases, with their highest activity detected outside the cells. Localization of A. laidlawii RNases was determined: almost 90% of the RNase activity was found to be associated with the membrane vesicles. Bioinformational analysis revealed homology between the nucleotide sequences of 14 Bacillus subtilis genes encoding the products with RNase activity and the genes of the mycoplasmas under study. Amino acid sequences of 4 A. laidlawii proteins with ribonuclease activity and the Bsn RNase were also established. © 2014 Pleiades Publishing, Ltd