66 research outputs found
Outcome of sleepiness and fatigue scores in obstructive sleep apnea syndrome patients with and without restless legs syndrome after nasal CPAP
Comparative X-ray analysis of the unliganded fosfomycin-target MurA
MurA, an essential enzyme for the synthesis of the bacterial cell wall, follows an induced-fit mechanism. Upon substrate binding, the active site forms in the interdomain cleft, involving movements of the two domains of the protein and a reorientation of the loop Pro112-Pro121. We compare two structures of un-liganded MurA from Enterobacter cloacae: a new orthorhombic form, solved to 1.80 Å resolution, and a monoclinic form, redetermined to 1.55 Å resolution. In the monoclinic form, the loop Pro112-Pro121 stretches into solvent, while in the new form it adopts a winded conformation, thereby reducing solvent accessibility of the critical residue Cys115. In the interdomain cleft a network of 27 common water molecules has been identified, which partially shields negative charges in the cleft and stabilizes the orientation of catalytically crucial residues. This could support substrate binding and ease domain movements. Near the hinge region an isoaspartyl residue has been recognized, which is the product of post-translational modification of the genetically encoded Asn67-Gly68. The homogenous population with L-isoaspartate in both structures suggests that the modification in Enterobacter cloacae MurA is not a mere aging defect but rather the result of a specific in vivo process.bacterial cell wall; induced-fit mechanism; solvent network; deamidation; isoaspartate formatio
Evidence that the fosfomycin target Cys<sup>115</sup> in UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) is essential for product release
A new view of the mechanisms of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate states
Structural studies of tau protein and Alzheimer paired helical filaments show no evidence for -structure
Méthodes algébriques pour les langages formels (applications à la logique et à la dynamique symbolique)
PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF
Evaluation der Sicherheit und Wirksamkeit von LASIK-Behandlungen mit verkürztem UV-Riboflavin-Crosslinking im Vergleich zur konventionellen LASIK bei hoher Myopie und myopem Astigmatismus - XtraLASIK
Intraindividueller Vergleich zweier Fragmentierungsmuster bei Femtosekundenlaser-assistierter Kataraktchirurgie
Visuelle Ergebnisse 3 Monate nach bilateraler Implantation einer Tiefenschärfen-Intraokularlinse
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