All-Optical Production of Chromium Bose-Einstein Condensates

We report on the production of ^52Cr Bose Einstein Condensates (BEC) with an all-optical method. We first load 5.10^6 metastable chromium atoms in a 1D far-off-resonance optical trap (FORT) from a Magneto Optical Trap (MOT), by combining the use of Radio Frequency (RF) frequency sweeps and depumping towards the ^5S_2 state. The atoms are then pumped to the absolute ground state, and transferred into a crossed FORT in which they are evaporated. The fast loading of the 1D FORT (35 ms 1/e time), and the use of relatively fast evaporative ramps allow us to obtain in 20 s about 15000 atoms in an almost pure condensate.Comment: 4 pages, 4 figure

Accumulation and thermalization of cold atoms in a finite-depth magnetic trap

We experimentally and theoretically study the continuous accumulation of cold atoms from a magneto-optical trap (MOT) into a finite depth trap, consisting in a magnetic quadrupole trap dressed by a radiofrequency (RF) field. Chromium atoms (52 isotope) in a MOT are continuously optically pumped by the MOT lasers to metastable dark states. In presence of a RF field, the temperature of the metastable atoms that remain magnetically trapped can be as low as 25 microK, with a density of 10^17 atoms.m-3, resulting in an increase of the phase-space density, still limited to 7.10^-6 by inelastic collisions. To investigate the thermalization issues in the truncated trap, we measure the free evaporation rate in the RF-truncated magnetic trap, and deduce the average elastic cross section for atoms in the 5D4 metastable states, equal to 7.0 10^-16m2.Comment: 9 pages, 10 Figure

Averaging out magnetic forces with fast rf-sweeps in an optical trap for metastable chromium atoms

We introduce a novel type of time-averaged trap, in which the internal state of the atoms is rapidly modulated to modify magnetic trapping potentials. In our experiment, fast radiofrequency (rf) linear sweeps flip the spin of atoms at a fast rate, which averages out magnetic forces. We use this procedure to optimize the accumulation of metastable chomium atoms into an optical dipole trap from a magneto-optical trap. The potential experienced by the metastable atoms is identical to the bare optical dipole potential, so that this procedure allows for trapping all magnetic sublevels, hence increasing by up to 80 percent the final number of accumulated atoms.Comment: 4 pages, 4 figure

Optimized loading of an optical dipole trap for the production of Chromium BECs

We report on a strategy to maximize the number of chromium atoms transferred from a magneto-optical trap into an optical trap through accumulation in metastable states via strong optical pumping. We analyse how the number of atoms in a chromium Bose Einstein condensate can be raised by a proper handling of the metastable state populations. Four laser diodes have been implemented to address the four levels that are populated during the MOT phase. The individual importance of each state is specified. To stabilize two of our laser diode, we have developed a simple ultrastable passive reference cavity whose long term stability is better than 1 MHz

Radio-frequency induced ground state degeneracy in a Chromium Bose-Einstein condensate

We study the effect of strong radio-frequency (rf) fields on a chromium Bose-Einstein condensate (BEC), in a regime where the rf frequency is much larger than the Larmor frequency. We use the modification of the Land\'{e} factor by the rf field to bring all Zeeman states to degeneracy, despite the presence of a static magnetic field of up to 100 mG. This is demonstrated by analyzing the trajectories of the atoms under the influence of dressed magnetic potentials in the strong field regime. We investigate the problem of adiabaticity of the rf dressing process, and relate it to how close the dressed states are to degeneracy. Finally, we measure the lifetime of the rf dressed BECs, and identify a new rf-assisted two-body loss process induced by dipole-dipole interactions.Comment: 4 pages, 4 figure

Tunneling control and localization for Bose-Einstein condensates in a frequency modulated optical lattice

The similarity between matter waves in periodic potential and solid-state physics processes has triggered the interest in quantum simulation using Bose-Fermi ultracold gases in optical lattices. The present work evidences the similarity between electrons moving under the application of oscillating electromagnetic fields and matter waves experiencing an optical lattice modulated by a frequency difference, equivalent to a spatially shaken periodic potential. We demonstrate that the tunneling properties of a Bose-Einstein condensate in shaken periodic potentials can be precisely controlled. We take additional crucial steps towards future applications of this method by proving that the strong shaking of the optical lattice preserves the coherence of the matter wavefunction and that the shaking parameters can be changed adiabatically, even in the presence of interactions. We induce reversibly the quantum phase transition to the Mott insulator in a driven periodic potential.Comment: Laser Physics (in press

The Effect of Epstein-Barr Virus Latent Membrane Protein 2 Expression on the Kinetics of Early B Cell Infection

Infection of human B cells with wild-type Epstein-Barr virus (EBV) in vitro leads to activation and proliferation that result in efficient production of lymphoblastoid cell lines (LCLs). Latent Membrane Protein 2 (LMP2) is expressed early after infection and previous research has suggested a possible role in this process. Therefore, we generated recombinant EBV with knockouts of either or both protein isoforms, LMP2A and LMP2B (Δ2A, Δ2B, Δ2A/Δ2B) to study the effect of LMP2 in early B cell infection. Infection of B cells with Δ2A and Δ2A/Δ2B viruses led to a marked decrease in activation and proliferation relative to wild-type (wt) viruses, and resulted in higher percentages of apoptotic B cells. Δ2B virus infection showed activation levels comparable to wt, but fewer numbers of proliferating B cells. Early B cell infection with wt, Δ2A and Δ2B viruses did not result in changes in latent gene expression, with the exception of elevated LMP2B transcript in Δ2A virus infection. Infection with Δ2A and Δ2B viruses did not affect viral latency, determined by changes in LMP1/Zebra expression following BCR stimulation. However, BCR stimulation of Δ2A/Δ2B cells resulted in decreased LMP1 expression, which suggests loss of stability in viral latency. Long-term outgrowth assays revealed that LMP2A, but not LMP2B, is critical for efficient long-term growth of B cells in vitro. The lowest levels of activation, proliferation, and LCL formation were observed when both isoforms were deleted. These results suggest that LMP2A appears to be critical for efficient activation, proliferation and survival of EBV-infected B cells at early times after infection, which impacts the efficient long-term growth of B cells in culture. In contrast, LMP2B did not appear to play a significant role in these processes, and long-term growth of infected B cells was not affected by the absence of this protein. © 2013 Wasil et al

Complex lithium ion dynamics in simulated LiPO3 glass studied by means of multi-time correlation functions

Molecular dynamics simulations are performed to study the lithium jumps in LiPO3 glass. In particular, we calculate higher-order correlation functions that probe the positions of single lithium ions at several times. Three-time correlation functions show that the non-exponential relaxation of the lithium ions results from both correlated back-and-forth jumps and the existence of dynamical heterogeneities, i.e., the presence of a broad distribution of jump rates. A quantitative analysis yields that the contribution of the dynamical heterogeneities to the non-exponential depopulation of the lithium sites increases upon cooling. Further, correlated back-and-forth jumps between neighboring sites are observed for the fast ions of the distribution, but not for the slow ions and, hence, the back-jump probability depends on the dynamical state. Four-time correlation functions indicate that an exchange between fast and slow ions takes place on the timescale of the jumps themselves, i.e., the dynamical heterogeneities are short-lived. Hence, sites featuring fast and slow lithium dynamics, respectively, are intimately mixed. In addition, a backward correlation beyond the first neighbor shell for highly mobile ions and the presence of long-range dynamical heterogeneities suggest that fast ion migration occurs along preferential pathways in the glassy matrix. In the melt, we find no evidence for correlated back-and-forth motions and dynamical heterogeneities on the length scale of the next-neighbor distance.Comment: 12 pages, 13 figure

J Clin Med

In cystic fibrosis (CF), cystic fibrosis transmembrane regulator (CFTR) dysfunction leads to digestive disorders that promote intestinal inflammation and dysbiosis enhancing gastrointestinal symptoms. In pancreatic insufficiency CF patients, both intestinal inflammation and dysbiosis, are associated with an increase in the fecal calprotectin (FC) level. However, associations between the FC level, gastrointestinal symptoms, and quality of life (QoL) remain poorly studied. We aimed to assess such associations in pancreatic insufficiency CF children. The FC level was measured in pancreatic insufficiency CF children's stool samples. Children and their parents completed two questionnaires: The Gastrointestinal Symptoms Scales 3.0-PedsQL(TM) and the Quality of Life Pediatric Inventory 4.0-PedsQL(TM). Lower scores indicated worse symptomatology or QoL. Thirty-seven CF children were included. A FC level above 250 µg/g was associated with worse gastrointestinal symptoms and QoL scores. The FC level was inversely correlated with several gastrointestinal scores assessed by children (i.e., Total, "Heart Burn Reflux", "Nausea and Vomiting", and "Gas and Bloating"). Several QoL scores were correlated with gastrointestinal scores. The FC level was weakly associated with clinical parameters. Some gastrointestinal and QoL scores were related to disease severity associated parameters. In CF, the FC level, biomarker previously related to intestinal inflammation and dysbiosis, was associated with worse digestive symptoms and QoL scores