The search for a permanent electric dipole moment using 129Xe and 3He

Time reversal and parity non‐invariant interactions within an atom naturally give rise to an atomic permanent electric dipole moment (PEDM). For noble gas atoms, the size of such a PEDM scales as Z2 and higher powers of Z depending on the actual manifestation of T non‐invariance, most importantly a distribution of electric dipole moment within the nucleus (Schiff Moment) and a T‐odd tensor interaction between the nucleus and atomic electrons. We have developed techniques to simultaneously measure the PEDMs of 129Xe and 3He in a single cell in order to mitigate systematic effects due to leakage currents and common mode problems such as magnetic field and time base noise. The philosophy of our approach is that PEDM of 3He is negligible compared to that of 129Xe and thus we use the 3He as a ‘‘magnetometer’’ and monitor of systematic effects. Sensitivity of ≊10−25 e‐cm per day has been demonstrated in preliminary work using a free‐induction decay technique.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87703/2/73_1.pd

The search for a permanent electric dipole moment using 129Xe and 3He

Time reversal and parity non‐invariant interactions within an atom naturally give rise to an atomic permanent electric dipole moment (PEDM). For noble gas atoms, the size of such a PEDM scales as Z2 and higher powers of Z depending on the actual manifestation of T non‐invariance, most importantly a distribution of electric dipole moment within the nucleus (Schiff Moment) and a T‐odd tensor interaction between the nucleus and atomic electrons. We have developed techniques to simultaneously measure the PEDMs of 129Xe and 3He in a single cell in order to mitigate systematic effects due to leakage currents and common mode problems such as magnetic field and time base noise. The philosophy of our approach is that the PEDM of 3He is negligible compared to that of 129Xe and thus we use the 3He as a ‘‘magnetometer’’ and monitor of systematic effects. Sensitivity of ≊10−25 e‐cm per day has been demonstrated in preliminary work using a free induction decay technique.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87730/2/84_1.pd

Construcción y monitorización del Laboratorio REVen para el estudio del impacto de las ventanas integrando eficiencia energética y calidad ambiental interior

REVen Project: “Energy retrofitting of social housing, using innovative window products satisfying CE marking requirements”. (BIA2014-56650-JIN) aims to carry out a comprehensive analysis of window impact on aspects related to energy efficiency and indoor environmental quality. REVen Laboratory has been built to characterize the energy flows and internal environmental conditions. This article describes the construction and monitoring of this laboratory analyzing the data of its first year of operation. Results can affirm that in the conditions of the test, a significant improvement on thermal comfort is observed obtaining an annual energy saving of 25 %.El proyecto REVen “Rehabilitación energética de viviendas sociales, aplicando productos innovadores de ventana con marcado CE” (BIA2014-56650-JIN), tiene como objetivo realizar un análisis integral del impacto de la ventana en los aspectos relativos a eficiencia energética y calidad ambiental. Para caracterizar los flujos de energía y las condiciones ambientales internas se ha construido el Laboratorio REVen. Este artículo describe la construcción y la monitorización de este laboratorio analizando los datos de su primer año de funcionamiento. Los resultados permiten afirmar que se logra una mejora significativa del confort térmico obteniendo un ahorro de energía anual del 25 %

Recombinase-based conditional and reversible gene regulation via XTR alleles

Synthetic biological tools that enable precise regulation of gene function within in vivo systems have enormous potential to discern gene function in diverse physiological settings. Here we report the development and characterization of a synthetic gene switch that, when targeted in the mouse germline, enables conditional inactivation, reports gene expression and allows inducible restoration of the targeted gene. Gene inactivation and reporter expression is achieved through Cre-mediated stable inversion of an integrated gene-trap reporter, whereas inducible gene restoration is afforded by Flp-dependent deletion of the inverted gene trap. We validate our approach by targeting the p53 and Rb genes and establishing cell line and in vivo cancer model systems, to study the impact of p53 or Rb inactivation and restoration. We term this allele system XTR, to denote each of the allelic states and the associated expression patterns of the targeted gene: eXpressed (XTR), Trapped (TR) and Restored (R)

Supplementation with cyanidin and delphinidin mitigates high fat diet-induced endotoxemia and associated liver inflammation in mice

Consumption of high fat diets (HFD) and the associated metabolic endotoxemia can initiate liver inflammation and lipid deposition that with time can progress to non-alcoholic fatty liver disease (NAFLD). We previously observed that 14 weeks supplementation with the anthocyanidins cyanidin and delphinidin mitigated HFD-induced metabolic endotoxemia and liver insulin resistance, steatosis, inflammation and oxidative stress. This work investigated if a 4-week supplementation of mice with a cyanidin- and delphinidin-rich extract (CDRE) could mitigate or reverse HFD (60% calories from lard fat)-induced liver steatosis and inflammation. After a first 4-weeks period on the HFD, mice showed increased endotoxemia and activation of liver proinflammatory signaling cascades. Supplementation with CDRE between weeks 4 and 8 did not mitigate liver steatosis or the altered lipid and glucose plasma levels. However, CDRE supplementation reverted HFD-induced metabolic endotoxemia, in parallel with the mitigation of the overexpression of hepatic TLR2 and TLR4, and of the activation of: (i) NF-κB, (ii) AP-1 and upstream mitogen-activated kinases p38 and ERK1/2, and (iii) HIF-1. Thus, even a short-term consumption of cyanidin and delphinidin could help mitigate the adverse consequences, i.e. metabolic endotoxemia and associated liver inflammation, triggered by the regular consumption of diets rich in fat.Fil: Cremonini, Eleonora. University of California at Davis; Estados UnidosFil: Iglesias, Dario Ezequiel. University of California at Davis; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Matsukuma, Karen E.. University of California at Davis; Estados UnidosFil: Hester, Shelly N.. No especifíca;Fil: Wood, Steven M.. No especifíca;Fil: Bartlett, Mark. No especifíca;Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Oteiza, Patricia I.. University of California at Davis; Estados Unido