10 research outputs found
MONO- AND MIXED-HERPESVIRUS INFECTIONS: ASSOCIATION WITH CLINICAL SYNDROMES OF IMMUNODEFICIENCY
In recent years, the number of hard diagnosed, polysymptomatic and polysyndrome conditions and diseases caused by mono- and mixed-herpes infections has sharply increased in the practice of physicians of all specialties. The clinical aspects of the close attention of physicians, virologists and epidemiologists to this viral family are due not only to the onset of atypical forms of these infections, but also to the emergence of the relative new concept of βactive chronic atypical infectionβ caused by herpesviruses and in particular the Epstein-Barr virus. We observed 198 people of both sexes aged between 23 and 60 years, suffering from mono- and mixed herpetic infections (EBV, CMV, HSV 1/2, and HSV type 6). 36% of these, suffered from mono-herpesvirus infections, mixed-herpesvirus infections were diagnosed in 63.7% of cases. A study of functioning characteristics of antiviral protection system as well as defects and disorders in the system of interferons was carried out in patients suffering from various mono-, mixed herpes virus infections and bacterial co-infections. The main clinical syndromes associated with these herpetic infections, as well as prevailing nosological forms of concomitant diseases were revealed. The revealed clinical syndromes and functioning characteristics of an antiviral protection will allow developing of a conceptual, individualized, etio- and immunopathogenetic therapy
EARLY DIAGNOSTICS OF AUTOINFLAMMATORY DISORDERS ASSOCIATED WITH POST-VIRAL CHRONIC FATIGUE SYNDROME AND COGNITIVE IMPAIRMENTS IN CHRONIC MIXED HERPES VIRAL INFECTIONS
The steady increase in the number of autoimmune diseases and immune-mediated autoinflammatory processes causes an increased interest of doctors of all specialties in this topic and makes the issue of early detection of autoimmune disorders / autoimmune syndrome (AS) extremely urgent. These disorders often develop against the backdrop of an atypical stream of chronic active viral infections caused by persistent viruses, in particular those of the Herpesviridae family, and remain undiagnosed due to polysymptomatic disease, and various βclinical masksβ of the disorders caused by them. The semi-quantitative method developed by us for screening assessment of the content of autoantibodies in the blood serum of patients suffering from ACAI caused by herpes viruses using the ELISA method (Immunodot) is a highly specific screening method that can allow for an objective assessment of the dynamics of the autoimmune process, as well as control the effectiveness of the ongoing complex antiviral and immunomodulatory therapy. The detection of autoantibodies of various specificity in the blood serum of patients suffering from an atypical chronic active infection caused by herpes viruses (ACAI) is an early diagnostic marker, necessary, first of all, to identify autoimmune pathology of the nervous system, which is associated with a long course of the active mixed herpes-viral process
Peculiarities of post-viral chronic fatigue syndrome associated with mild cognitive decline in patients with atypical chronic active herpesvirus infections
According to modern ideas, changes in the functioning of the immune system affect the immune processes in the nervous system, contributing to the development of neuro-immuno-inflammation and thereby indirectly affect the rate of progression of neurodegenerative processes. The aim of our study was to investigate the prevalence of post-viral chronic fatigue syndrome and cognitive impairment (aMCI) among patients with atypical, chronic active herpesvirus infections (ACA-HVI).Under our supervision were 126 patients of both sexes aged 18 to 60 years with ACA-HVI.It was established that mono-EBV infection affects 27.7%; mixed EBV infection is observed in 72.3% of patients. When assessing cognitive functioning using CGI, MMSE scales, the incidence of aMCI was found to be 68.3%: with mixed HVI β 87.4%, with mono HVI β 38.8%. During the study, significant limitations were identified in the use of standard scales due to the impossibility of conducting a comprehensive assessment of clinical status parameters and cognitive dysfunctions, as well as correlation of these parameters and assessment of dynamics of the immunocorrection. To achieve this goal the Scale of assessment of the criterion clinical symptoms of patients with ACA-HVI with CFS was used. It was shown that in mixed-HVI, the severity of symptoms exceeded the severity of symptoms of patients with mono-HVI and was 52.7 (43.1-62.2) and 38.0 (31.9-42.8) points, respectively (p > 0.05). Thus, it was found that patients suffering from mixed HVI have more pronounced, severe manifestations of CFS and aMCI, which are 1.5 times higher than similar manifestations in patients with mono-HVI, significantly reducing the quality of life of these patients, worsening their social adaptation.Prolonged persistence of herpes viruses in immune-compromised people creates conditions for constant antigenic stimulation and immune imbalance with the onset of secondary immunodeficiency or clinical manifestation of existing primary disorders in the immune system, which creates the prerequisites for the development of neuro-immuno-inflammatory changes in nervous system, followed by the formation of clinical manifestations of ME/CFS with different cognitive impairments that may be classified as aMCI
The local interferon-corrective therapy in children with congenital cleft lip and palate, suffering from the recurrent respiratory infections
It is known that children with congenital cleft lip and palate are suffering from recurrent respiratory infections, which worsen the state of their health, and also complicate the results of reconstructive surgical treatment. The aim of the study was to detect defects of mucosal immunity in children with congenital cleft lip and palate, suffering from recurrent respiratory infections, and to create the program of local interferon corrective therapy with an assessment of its effectiveness. The studies included 56 children from the age of 1 to 3 years. Three groups of children were formed: group 1 β 26 children with congenital cleft lip and palate (antibiotic therapy); group 2 β 30 children with congenital cleft lip and palate (antibiotic therapy + local interferon therapy), group 3 β the control group. The clinical examination included a medical history, an assessment of the symptoms of recurrent episodes of acute respiratory infections and exacerbations of chronic infections. Microbiological studies were performed using standard methods. The status of local immunity was detected: the concentrations of secretory IgA, cytokines IL-17, IL-4, IL-6, IL-1Ξ², IFNΞ³ in the oral fluid were tested by ELISA. Results of the study established that in group 1 and group 2 clinical criteria of immunodeficiency with an infectious syndrome were revealed: repeated acute respiratory viral infections from 10 or more times a year, complicated by frequent exacerbations of chronic bacterial infection (up to 10 or more per year). Assessment of the state of local immunity in children with congenital cleft lip and palate revealed a lack of sIgA compared with the control group. Before treatment in group 2 oral fluid level of IL-17, IL-6 were statistically significant increase (p < 0.05); the results of the study also established increase in the level of IL-1Ξ² and a decrease in anti-inflammatory IL-4 and regulatory IFNΞ³ relative to the control group (p > 0.05). After complex treatment with the inclusion of local interferon therapy in group 2 the appearance of sIgA, increase in the concentration of IL-4, IL-1Ξ² and a decrease IL-17 in oral fluid were observed (p > 0.05). The concentrations of IL-6, IFNΞ³ did not change (p > 0.05). After treatment in group 2 there were a decrease in exacerbations of chronic upper respiratory tract infection and in frequency of acute respiratory viral infections compared with group 1 (p < 0.05). Positive clinical efficacy of local interferon therapy (the gel of recombinant IFNΞ±2b in combination with oxidants β Viferon gel) in the process of staged rehabilitation of children with congenital cleft lip and palate has a protective clinical effect in reducing the frequency of acute respiratory viral infections, reducing the number of postoperative complications, reducing hospital stay, duration of antibacterial therapy and the number of exacerbations of chronic bacterial infection
The defect of medical care is a crime committed by a doctor
The variant of the forensic medical assessment of the quality of medical care was presented, this defect is a criminal offence, measures to prevent such offenses are necessaryΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ Π²Π°ΡΠΈΠ°Π½Ρ ΡΡΠ΄Π΅Π±Π½ΠΎ-ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠΉ ΠΏΠΎΠΌΠΎΡΠΈ, ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΡΠΉ Π΄Π΅ΡΠ΅ΠΊΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ ΠΊΠ°ΠΊ ΠΏΡΠ΅ΡΡΡΠΏΠ»Π΅Π½ΠΈΠ΅, ΠΊΠΎΡΠΎΡΠΎΠ΅ Π΄ΠΈΠΊΡΡΠ΅Ρ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ ΠΌΠ΅Ρ ΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠΈ ΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ
ΠΏΡΠ°Π²ΠΎΠ½Π°ΡΡΡΠ΅Π½ΠΈ
ΠΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΡ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΈ ΠΏΠ΅ΡΡΠΈΡΡΠΈΡΡΡΡΠΈΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ
Children with chronic diseases of respiratory tract were investigated for IgM and IgG serum antibodies to viruses (Herpes simplex virus, Epstein-Barr virus, Cytomegalovirus) and bacteria (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumonia) by ELISA and for telomere length of their lymphocytes by fluorescent hybridization in situ followed by flow cytomerty (FlowFISH). The purpose of the study was to compare the lymphocyte telomere length of these seropositive and seronegative children. Regression analysis and analysis of percent distribution relative to the regression line have compensated age differences between seropositive and seronegative groops and demonstrated shorter telomeres in lymphocytes of children those have serum antibodies to viruses in all combinations (including combinations with antibodies to bacteria) in contrast to seronegative children of the same age.ΠΠ΅ΡΠΈ Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π±ΡΠ»ΠΈ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ Π½Π° Π½Π°Π»ΠΈΡΠΈΠ΅ IgM ΠΈ IgG Π°Π½ΡΠΈΡΠ΅Π» Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² (Herpes simplex, Epstain-Barr, Cytomegalovirus) ΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumoniae) ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ELISA. ΠΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π΄Π»ΠΈΠ½Ρ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ in situ ΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (FlowFISH) Ρ ΡΠ΅Π»ΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Π΄Π»ΠΈΠ½Ρ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Ρ ΡΠ΅ΡΠΎΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΡΡ
ΠΈ ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ². ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΈ Π°Π½Π°Π»ΠΈΠ·Π° Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² (Π² ΠΏΡΠΎΡΠ΅Π½ΡΠ°Ρ
) ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»ΠΎ Π½ΠΈΠ²Π΅Π»ΠΈΡΠΎΠ²Π°ΡΡ Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡ ΡΠ΅ΡΠΎΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΠΎΠΉ ΠΈ ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏ ΠΈ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ, ΡΡΠΎ ΡΠ΅Π»ΠΎΠΌΠ΅ΡΡ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π΄Π΅ΡΠ΅ΠΉ, ΠΈΠΌΠ΅ΡΡΠΈΡ
Π°Π½ΡΠΈΡΠ΅Π»Π° ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡΡ
, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡΡ
Ρ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ ΠΏΡΠΎΡΠΈΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, ΠΊΠΎΡΠΎΡΠ΅ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ ΡΠΎΠ³ΠΎ ΠΆΠ΅ Π²ΠΎΠ·ΡΠ°ΡΡΠ°
Changes in the Structure of the Chromosomes of Lymphocytes during Persistent Infection in Children
Children with chronic diseases of respiratory tract were investigated for IgM and IgG serum antibodies to viruses (Herpes simplex virus, Epstein-Barr virus, Cytomegalovirus) and bacteria (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumonia) by ELISA and for telomere length of their lymphocytes by fluorescent hybridization in situ followed by flow cytomerty (FlowFISH). The purpose of the study was to compare the lymphocyte telomere length of these seropositive and seronegative children. Regression analysis and analysis of percent distribution relative to the regression line have compensated age differences between seropositive and seronegative groops and demonstrated shorter telomeres in lymphocytes of children those have serum antibodies to viruses in all combinations (including combinations with antibodies to bacteria) in contrast to seronegative children of the same age
ΠΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΡΡΡΠΊΡΡΡΡ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΈ ΠΏΠ΅ΡΡΠΈΡΡΠΈΡΡΡΡΠΈΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ
Children with chronic diseases of respiratory tract were investigated for IgM and IgG serum antibodies to viruses (Herpes simplex virus, Epstein-Barr virus, Cytomegalovirus) and bacteria (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumonia) by ELISA and for telomere length of their lymphocytes by fluorescent hybridization in situ followed by flow cytomerty (FlowFISH). The purpose of the study was to compare the lymphocyte telomere length of these seropositive and seronegative children. Regression analysis and analysis of percent distribution relative to the regression line have compensated age differences between seropositive and seronegative groops and demonstrated shorter telomeres in lymphocytes of children those have serum antibodies to viruses in all combinations (including combinations with antibodies to bacteria) in contrast to seronegative children of the same age.ΠΠ΅ΡΠΈ Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π±ΡΠ»ΠΈ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ Π½Π° Π½Π°Π»ΠΈΡΠΈΠ΅ IgM ΠΈ IgG Π°Π½ΡΠΈΡΠ΅Π» Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² (Herpes simplex, Epstain-Barr, Cytomegalovirus) ΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumoniae) ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ELISA. ΠΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π΄Π»ΠΈΠ½Ρ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ in situ ΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (FlowFISH) Ρ ΡΠ΅Π»ΡΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Π΄Π»ΠΈΠ½Ρ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Ρ ΡΠ΅ΡΠΎΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΡΡ
ΠΈ ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ². ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΈ Π°Π½Π°Π»ΠΈΠ·Π° Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² (Π² ΠΏΡΠΎΡΠ΅Π½ΡΠ°Ρ
) ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ»ΠΎ Π½ΠΈΠ²Π΅Π»ΠΈΡΠΎΠ²Π°ΡΡ Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡ ΡΠ΅ΡΠΎΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΠΎΠΉ ΠΈ ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏ ΠΈ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ, ΡΡΠΎ ΡΠ΅Π»ΠΎΠΌΠ΅ΡΡ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Π΄Π΅ΡΠ΅ΠΉ, ΠΈΠΌΠ΅ΡΡΠΈΡ
Π°Π½ΡΠΈΡΠ΅Π»Π° ΠΏΡΠΎΡΠΈΠ² Π²ΠΈΡΡΡΠΎΠ² Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡΡ
, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡΡ
Ρ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ ΠΏΡΠΎΡΠΈΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, ΠΊΠΎΡΠΎΡΠ΅ ΡΠ΅Π»ΠΎΠΌΠ΅Ρ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΡΠ΅ΡΠΎΠ½Π΅Π³Π°ΡΠΈΠ²Π½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ ΡΠΎΠ³ΠΎ ΠΆΠ΅ Π²ΠΎΠ·ΡΠ°ΡΡΠ°