Method of variational calculation of influence of the propulsion plants of forestry machines upon the frozen and thawing soil grounds

The forests, which grow in the conditions of complete expansion of the perpetually frozen ground, are unique forests in accordance with their taxational characteristics, quality indicators of the felled timber, and the ecological functions, which these forests perform in the nature. They are characterised by the low biological productivity, as well as by the high vulnerability due to climatological changes and human economic activities. It is fair to say that conservation of the permafrost is one of the main functions of the forests, which grow within the cryolithozone. Because of this, it is necessary to ensure special regimes for the forestry management and forest exploitation within the forests of the cryolithozone. We formulated the variational problem in order to determine influence of the changeability of the physical and mechanical properties of the thawing soil ground at the boundary with the permafrost ground. © 2019 SERSC

Renormalization of hole-hole interaction at decreasing Drude conductivity

The diffusion contribution of the hole-hole interaction to the conductivity is analyzed in gated GaAs/In$_x$Ga$_{1-x}$As/GaAs heterostructures. We show that the change of the interaction correction to the conductivity with the decreasing Drude conductivity results both from the compensation of the singlet and triplet channels and from the arising prefactor $\alpha_i<1$ in the conventional expression for the interaction correction.Comment: 6 pages, 5 figure

Apoptosis- and survival-related gene mRNA profile in peripheral blood leukocytes in children with acute EBV infectious mononucleosis

Acute EBV-associated mononucleosis develops mainly in children and in patients with functionally impaired immune system. Consequently, it may result in developing secondary immunodeficiency, neoplasms as well as diverse alterations in cell-mediated immune reaction. Despite extensively examining molecular mechanisms of EBV infection, it is also necessary seek for new molecular and genetic factors underlying pathogenesis of EBV-mediated mononucleosis and EBV-associated malignant cell transformation is necessary, which might be used in clinical practice to monitor clinical score as well as predictive parameters for EBV-associated complications such as immunocompromised conditions and neoplasms. Here, we proposed to use our splicing sensitive DNA microarrays to perform a comprehensive semi-quantitative mRNA expression analysis for major apoptosis- and survival-related signaling components in peripheral blood leukocytes collected from children with acute EBV infectious mononucleosis as well as during recovery period. Using such DNA microchips allowed to assess both total (denoted by Σ) and separate transcript expression resulting from alternative splicing. It was shown that the balance of mRNA levels in acute phase of EBV-infectious mononucleosis was shifted towards upregulated expression of anti-apoptotic factors and components of of NF-κB-linked pro-survival signaling able to profoundly augment apoptosis resistance. Moreover, some EBV-associated changes (BIM/BCL2L11-Σ, PUMA/ BBC3-NM_001127241, BID-Σ, CASP3-Σ, NFKB1-Σ, RELA-Σ) were in agreement with the data published before. In addition, we also found previously unknown changes in level of EBV-associated coding and noncoding transcripts (DCR1/ TNFRSF10C-NM_003841, DR5/TNFRSF10B-NR_027140, CASP6 beta/CASP6-NM_032992, CASP7-NM_033338). Analyzing their properties allowed to suggest that they play an important role in the pathogenesis of EBV-associated mononucleosis. However, at asymptomatic recovery stage, level of some mRNA expression was kept altered compared to healthy volunteers (DCR2/TNFRSF10D-NM_003840, CASP8-Σ, CASP3-Σ, BIM/BCL2L11-Σ, BCL2-NM_000633, MCL1-Σ, BCL-W/BCL2L2-Σ, BCL-XL/BCL2L1-NM_138578, BIRC2-NM_001166, XIAP-NM_001167, TRAF2-NM_021138, MAP3K14-Σ, NFKB1-Σ), which may point at postponed EBV-associated molecular consequences. On one hand, such changes may be due to long-lasting anti-EBV immune response, whereas, on the other hand, they might be influenced by EBV-associated factors facilitating virus persistence. Overall, we identified the molecular features predisposing to chronic course of EBV-infection. The data obtained further expand our understanding about the molecular pathogenetic mechanisms for EBV infectious mononucleosis

Expression analysis of apoptotic and survival genes in blood leukocytes of children with various forms of HHV-6 infection

Despite that human herpes virus type 6 (HHV-6) is extremely spread worldwide, molecular mechanisms of behind HHV-6 infection pathogenesis remain largely unexplored. No molecular markers were found linked to unfavorable course of HHV-6 infection which could allow to ease up selecting proper therapy and preventing development of complications. The aim of the study was to analyze expression of apoptosis and survival-related genes in blood leukocytes from 7–17-year-old children upon various forms of HHV-6 infection. The analysis was carried out by using DNA microarrays developed by us allowing to assess changes in expression level both of individual mRNAs and total gene set (-Σ). It was shown that during the acute phase of HHV-6 infection mRNA level was shifted toward pro-apoptotic factors. In the convalescence phase, most altered mRNA levels returned to normal. We have identified a set of mRNAs and genes whose expression level was significantly changed in acute disease phase. According to available data, these factors play an important role in regulation of studied signaling pathways. In order to search for HHV-6-associated factors, which markedly affect disease pattern of severe herpesvirus mixed infection, we analyzed significant changes of mRNA and genes expression levels in patients with severe HHV-6+EBV+CMV mixed infection and EBV+CMV mixed infection of moderate severity compared with healthy donors. The levels of 5 mRNAs (FAF1-NM_007051, DAPK2-NM_014326, CASP8AP2-NM_001137667, CASP8-NM_033356, BTK-NM_001287345) and 3 genes (FAS-Σ, Puma/BBC3-Σ, ITCH-Σ) were significantly increased in severe mixed infection comorbid with HHV-6 (EBV+CMV+HHV-6) but without HHV-6 (EBV+CMV) compared with healthy donors. Most of detected factors belong to Fas-mediated apoptosis pathway, and may be considered as candidate prognostic development factors of severe herpes virus infection involving HHV-6. This study profoundly extends existing understanding on molecular pathogenesis of HHV-6 infection involving apoptosis and pro-survival signaling pathways. Marked changes of mRNA and gene levels most likely contributed to the pathogenesis of HHV-6 as well as severe HHV-6+EBV+CMV mixed infection

ENERGY EFFICIENCY IMPROVING IN AN AIR CONDITIONING SYSTEM BASED ON IMPLEMENTATION OF A COLD STORAGE TANK

В статье рассматривается энергоэффективность применения аккумуляторов холода в системе холодоснабжения. Произведена оценка факторов, влияющих на объем бака аккумулятора холода. факторов, влияющих на объем бака аккумулятора холода.The article discusses the energy efficiency of using cold batteries in the cold supply system. The estimation of factors influencing the volume of the cold accumulator tank is given

Dissecting the role of single nucleotide polymorphism of lymphotoxin beta gene during pig domestication using bioinformatic and experimental approaches

Studies of wild and laboratory animals have revealed a trade-off between reproductive success and immunity. Therefore, it is likely that domestication favored selection of individuals with high reproductive performance but low immunity. The low responsiveness of the immune system could become hereditary through fixation of genes with “unfavorable” mutations in populations. The objectives of this work are: 1) determination of frequencies of genotypes and alleles of the rs340283541 SNP in the gene for the lymphotoxin beta (LTB) cytokine in pigs of domestic breeds and wild boars; 2) investigation of the expression of LTB mRNA in minipigs with different genotypes, and 3) bioinformational analysis of the putative functional role of the SNP. The frequency of the GG genotype in the wild boar sample was significantly lower than in the pooled sample of domestic pigs. The LTB mRNA expression rate in the lymph node of minipigs with genotype GG tended to increase (p &lt; 0.06) in comparison with carriers o allele A. The rs340283541 SNP occurs in a DNA motif highly conservative among 11 mammalian species; thus, it may be of functional significance. Context analysis shows that allele A has putative binding sites for  transcription factors BRN-2 and AP-1, whereas allele G has binding sites for transcription factors RFX1, ISGF3 (site ISRE), and USF expressed in cells of the immune system. Thus, pig domestication was accompanied by an increase in the frequency of the GG genotype for the rs340283541 SNP, occurring in the 3’ region of the LTB gene. It is likely that the GG genotype is associated with elevated LTB mRNA expression in the lymph node tissue. This increase may be related to the formation of binding sites for RFX1, ISRE, and USF and/or disruption of binding sites for BRN-2 and AP-1. A linkage disequilibrium between rs340283541 and another functionally significant mutation in LTB is also conceivable