Genomic organization and cytokine-mediated inducibility of the human TRIM-8/Gerp gene.

Cytokine signaling is negatively regulated by a set of SH2 domain-containing proteins, the Suppressors of Cytokine Signaling (SOCS) acting as intracellular modulators. Experimental evidence indicates that SOCS gene expression is induced by cytokines and pro-inflammatory stimuli and is highly controlled both at transcription and translation level. Furthermore, SOCS proteins appear rapidly degraded inside the cells, mostly controlling their stability by interacting with specific molecules such as elongin B and C. It has been shown that SOCS-1/JAB, a member of the SOCS family, interacts with TRIM-8/Gerp, a new ring protein specifically binding SOCS-1 recombinant polypeptide in-vitro and in-vivo. Trim-8/Gerp, transcribes a 3.0-kb mRNA, spans 551 AA and is highly conserved during evolution. In addition, it can be induced by IFN-γ in epithelial and lymphoid cells and is expressed mostly ubiquitously in murine and human tissues. Here in this report we present the genomic organization of this new SOCS-1 interactor, and we add new tools for extending investigation of the complex mechanism that undergoes negatively regulation of cytokine signaling

Etude biochimique de la pulpe dentaire de veau

A biochemical study of dental pulp of calves has been performed concerning:a) peroxydabilityb) A, E, C vitamins contentc) glutation (GSH) contentd) presence of paramagnetic compoundse) phosphorylation ratioThe dental pulp from incisives of 5 months old calves has been preserved. Immediately after decapitation the pulp was immersed in liquid nitrogen. Chromatographie (HPLC) and spectroscopic (NMR-ESR) techniques have been used.GSH in dental pulp are present and dosable (4.56 +/-0.08 n moles/mg prot.) and GSSG (1.05 +/-0.01 n moles/mg prot.).Because of blood traces in the extracted pulps, the AA. have determined the hemoglobin (Hb) dosage and GSH of erythrocytary derivation (Fig. 1).After deduction of GSH of erythrocytary derivation, the GSH really present in the pulp was 4.41 n moles/mg prot. and the GSSG was 0.90 n moles/mg prot.Peroxydability of the dental pulp has been evaluated with Lowry method with dental pulp homogenate and rat liver homogenate (see Table 1).The ESR spectre shows 4 resonances with the following values: g. 2.24 - 2.04 - 2.00 - 1.97; there are some free intermediary radicals (gr. - 2.00) (Fig.2).The NMR spectre shows the presence of ATP (0.22 n moles/g) of inorganic phosphate (16.58 n moles g) (Fig.3).The pulp seems to have a lot of antioxydant factors. The next researches will be to study E, A and C vitamins concentrations. This high presence of GSH and GSSG may be an embryonary peculiarity.Une étude biochimique de la pulpe dentaire a été entreprise pour étudier les propriétés antioxydantes et leurs interactions avec le métabolisme énergétique et les équilibres redox de la pulpe dentaire de veau. Le GSH réduit et oxydé (GSSG) a été mesuré et la spectroscopie avec ESR (Electron Spin Résonance) a été utilisée pour la recherche des substances paramagnétiques et la résonance magnétique nucléaire (NMR) pour la détermination des métabolites phosphorylés de petit poids moléculaire. Après la soustraction de la quantité de GSH érythrocytaire, le GSH présent dans la pulpe est resté identique 4,41 n moles/mg prot. (GSH) et 0.90 n moles/mg n moles/mg prot. (GSSG). La péroxydation lipidique de la pulpe dentaire a été étudiée. Le spectre ESR montre 4 résonances, respectivement de valeurs de G de 2.24, 2.04, 2.00, 1.97.L’analyse des résultats montre l’existence d’une petite quantité de radicaux libres intermédiaires (g-2.00) dépendant du métabolisme tissulaire. Le spectre NMR a montré la présence d’ATP (0.22 n moles/g) et de phosphate inorganique (16.50 n moles/g)

Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.Chemistry and Chemical Biolog