DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae

The ability of yeast DNA polymerase mutant strains to carry out repair synthesis after UV irradiation was studied by analysis of postirradiation molecular weight changes in cellular DNA. Neither DNA polymerase alpha, delta, epsilon, nor Rev3 single mutants evidenced a defect in repair. A mutant defective in all four of these DNA polymerases, however, showed accumulation of single-strand breaks, indicating defective repair. Pairwise combination of polymerase mutations revealed a repair defect only in DNA polymerase delta and epsilon double mutants. The extent of repair in the double mutant was no greater than that in the quadruple mutant, suggesting that DNA polymerases alpha and Rev3p play very minor, if any, roles. Taken together, the data suggest that DNA polymerases delta and epsilon are both potentially able to perform repair synthesis and that in the absence of one, the other can efficiently substitute. Thus, two of the DNA polymerases involved in DNA replication are also involved in DNA repair, adding to the accumulating evidence that the two processes are coupled

Thermionic research and development program Final report

Rhenium electrode material investigation and performance studies of low temperature cesium vapor thermionic converter

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

The experiments conducted to determine the heat resistance of Bacillus megaterium ATCC 6458 at 90 and 100 C were completed. Estimates from replicate experiments at eight percent relative humidities (less than 0.001 to 100% RH) for each temperature were computed. A Bacillus cereus strain with high heat resistance was cultured and the resistance determined in phosphate buffer (D sub 121.1 = 2.16 min and z = 8.7 C). The profile of the dry heat resistance of B. megaterium is summarized and the most resistant condition to the three spores (Bacillus subtilis var. niger, ATCC 29669, and Bacillus stearothermophilus, strain 1518) is compared

The status of environmental satellites and availability of their data products

The latest available information about the status of unclassified environmental satellite (flown by the United States) and their data products is presented. The type of environmental satellites discussed include unmanned earth resource and meteorological satellites, and manned satellites which can act as a combination platform for instruments. The capabilities and data products of projected satellites are discussed along with those of currently operating systems

Thermionic research and development program Final report, 15 Jul. 1966 - 15 Jan. 1968

Thermionic research and development program - improvement of performance of low emitter temperature cesium vapor thermionic converter

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

Dry heat treatment is specified as the preferred means for the terminal sterilization of spacecraft and for decontamination of spacecraft components. The presence of organisms highly resistant to dry heat in soil and fallout around assembly and industrial manufacturing areas is shown. The dry heat survival characteristics of the Cape Kennedy isolate 4-6 B. brevis spores is demonstrated. The presence of hardy organisms from soil samples obtained from geographical areas of the United States is shown. A resistant fraction appears to occur in low numbers in a soil sample. The heat resistance characteristics of 4-6 B. brevis and B. subtilis var. niger spores are compared. Their morphological characteristics are compared by scanning electron microscopy

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

Statistical techniques which have relevance to studies on the thermal inactivation on bacterial spores are discussed

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

The heat resistance of Bacillus subtilis var. niger was measured from 85 to 125 C using moisture levels of % RH or = 0.001 to 100. Curves are presented which characterize thermal destruction using thermal death times defined as F values at a given combination of three moisture and temperature conditions. The times required at 100 C for reductions of 99.99% of the initial population were estimated for the three moisture conditions. The linear model (from which estimates of D are obtained) was satisfactory for estimating thermal death times (% RH or = 0.07) in the plate count range. Estimates based on observed thermal death times and D values for % RH = 100 diverged so that D values generally gave a more conservative estimate over the temperature range 90 to 125 C. Estimates of Z sub F and Z sub L ranged from 32.1 to 58.3 C for % RH of or = 0.07 and 100. A Z sub D = 30.0 was obtained for data observed at % RH or = 0.07

Ecology and Thermal Inactivation of Microbes in and on Interplanetary Space Vehicle Components

Spores of Bacillus subtilis var. niger were heat treated in aqueous suspension at 90 C, and observed for morphological changes and loss of viability. The 5 logs reduction that occurred in broth at 90 min required 210 min in buffered water. Five characteristic changes observed after spores were exposed 120 min at 90 C in buffered water were: (1) 90% loss of spore viability, (2) 5% stainability, (3) 76% increase in spore size (as observed by scanning electron microscopy), (4) 21% of spore areas remaining refractile, and (5) an increase of 77% in packed cell volume (PCV). Stainability and PCV changes were recognized only after secondary exposure in broth. Extended heat exposure (3 h at 90 C) resulted in 99% loss of spore viability and 99% loss of stainability. After 4 hours of heat exposure, 90% of the cells disintegrated. These results suggest that early germinal changes occurr concurrently with the early changes in the heat susceptibility of dormant spores