Expression profiles of Interferon-tau Stimulated Genes in milk cells in pregnant dairy cows

17th Annual Conference of the European-Society-of-Domestic-Animal-Reproduction (ESDAR) -- SEP 12-14, 2013 -- Univ Bologna, Bologna, ITALYWOS: 000323318700033…European Soc Domest Anim Reprod (ESDAR), Fatro, Sexing Technologies, Virbac, Minitube, Michelson Prize&Grants, Biophos, Proiser, Chemometec, IMV Technologies, Alma Mater Studiorum Univ Bologna, Casa Editrice Ambrosiana, Assogen

Expression profiles of Interferon-Tau Stimulated Genes (ISGs) in Peripheral Blood Leucocytes (PBLs) and milk cells in pregnant dairy cows [Sütçü gebe İneklerde Periferal Kan Lökositleri (PBLs) ve Süt Hücrelerindeki Interferon-Tau Stimulated Genlerinin (ISGs) ekspresyon profili]

In previous reports, it was indicated that measurement of activity of Interferon-tau Stimulated Genes (ISGs) in Peripheral Blood Leucocytes (PBLs) may be used as an alternative early pregnancy detection method in dairy cows. However, there are no data showing the expression profiles of ISGs in other body fluids containing leucocytes such as milk. In the present study, it was hypothesized that leucocytes in milk samples may reflect the increases in expression profiles of ISGs as shown in PBLs. For this purpose, nine pregnant lactating Holstein cows were used. Insemination day was accepted as day zero (day 0). Blood and milk samples were collected on day 0 and 18 after insemination for cell isolation. Total RNA was extracted from isolated cells and converted to cDNA. Steady state levels of Interferon-tau Stimulated Gene 15 (ISG15), Myxovirus (influenza virus) resistance 1 (MX1) and 2 (MX2) mRNA transcripts were assayed by using real-time reverse transcriptase PCR. Relative Expression Software Tool (REST2009) was used for statistical analyses. There was no statistical significant difference for expression levels of ISG15, MX1 and MX2 mRNAs between days 0 and 18 in milk samples. However, when compared to day 0, levels of ISG15 and MX2 transcripts were increased 6.97±0.68 fold and 5.84±1.27 fold on day 18 in PBLs in pregnant cows, respectively (P<0.05). According to this result, it may be suggested that milk cells are not suitable measurement of expression profiles of ISGs to detect early pregnancy in lactating dairy cows

Concensus and comprehensive linkage maps of bovine chromosome 24

The definitive version is available at www.blackwell-synergy.comThis study describes development of a consensus genetic linkage map of bovine chromosome 24 (BTA24). Eight participating laboratories contributed data for 58 unique markers including a total of 25 409 meioses. Eighteen markers, which were typed in more than one reference population, were used as potential anchors to generate a consensus framework map. The framework map contained 16 loci ordered with odds greater than 1000:1 and spanned 79.3 cM. Remaining markers were included in a comprehensive map relative to these anchors. The resulting BTA24 comprehensive map was 98.3 cM in length. Average marker intervals were 6.1 and 2.5 cM for framework and comprehensive maps, respectively. Marker order was generally consistent with previously reported BTA24 linkage maps. Only one discrepancy was found when comparing the comprehensive map with the published USDA-MARC linkage map. Integration of genetic information from different maps provides a high-resolution BTA24 linkage map