An insight into curcumin-based photosensitization as a promising and green food preservation technology

Consumer awareness on the side effects of chemical preservatives has increased the demand for natural preservation technologies. An efficient and sustainable alternative to current conventional preservation techniques should guarantee food safety and retain its quality with minimal side effects. Photosensitization, utilizing light and a natural photosensitizer, has been postulated as a viable and green alternative to the current conventional preservation techniques. The potential of curcumin as a natural photosensitizer is reviewed in this paper as a practical guide to develop a safe and effective decontamination tool for industrial use. The fundamentals of the photosensitization mechanism are discussed, with the main emphasis on the natural photosensitizer, curcumin, and its application to inactivate microorganisms as well as to enhance the shelf life of foods. Photosensitization has shown promising results in inactivating a wide spectrum of microorganisms with no reported microbial resistance due to its particular lethal mode of targeting nucleic acids. Curcumin as a natural photosensitizer has recently been investigated and demonstrated efficacy in decontamination and delaying spoilage. Moreover, studies have shown the beneficial impact of an appropriate encapsulation technique to enhance the cellular uptake of photosensitizers, and therefore, the phototoxicity. Further studies relating to improved delivery of natural photosensitizers with inherent poor solubility should be conducted. Also, detailed studies on various food products are warranted to better understand the impact of encapsulation on curcumin photophysical properties, photo-driven release mechanism, and nutritional and organoleptic properties of treated foods

Анализ эффективности тромбопрофилактики у беременных с мезенхимальной дисплазией

the analysis of the effectiveness of thromboprophylaxis among pregnant women with mesenchymal dysplasia was carried out. It was concluded that it reduces the risk of thrombohemorrhagic complications and accordingly the economic costs of medical care.проведен анализ эффективности тромбопрофилактики у беременных с мезенхимальной дисплазией, при использовании которой во время беременности у таких пациенток снижается риск возникновения тромбогеморрагических осложнений и, следовательно, уменьшаются финансовые затраты

MOLECULAR-GENETIC CHARACTERISTICS OF PRIMARY TUMOR AND METASTATIC LYMPHATIC NODES IN BREAST CANCER

The purpose of systemic treatment in patients with breast cancer is based largely on the molecular characteristics of the primary tumor, but many clinical recommendations suggest also the study of metastatic nodes with an assessment of their receptor status (estrogen receptor ER, progesterone receptor RP, human epidermal growth factor receptor 2 Her2/neu). This is due to the fact that according to numerous studies, the discrepancy between the status of the primary tumor and the secondary nodes can reach high rates: 3–54 % for ER, 5–78 % for RP, and 0–34 % for Her2/neu. At the same time, more and more data actively demonstrate the imperfection of immunohistochemical analysis and the need to study additional parameters to improve the quality of diagnosis of patients with breast cancer. Material and methods. A morphological and immunohistochemical study of the tumor tissue of the primary node and axillary lymph nodes was performed in 199 patients with breast cancer (T1-3N0-3M0) using standard methods, and RT-PCR was also studied with the expression of 24 genes. Results. The incidence of differences between the molecular phenotypes of the main tumor and metastatic axillary lymph nodes was 26 (26 %) of 99 cases. Most often, differences were noted in cases of breast cancer with luminal A type – 13 cases (50 %). According to the results of a comparative PCR analysis of tissue samples from the primary tumor and metastatic regional lymph nodes, only the expression of the CD68, ERSR1, GRB7 and MMD11 receptors was statistically significant. Conclusion. The results indicate the need for an integrated approach and additional methods for the diagnosis of breast cancer, which will undoubtedly improve the quality of planning and the effectiveness of systemic treatment in patients with breast cancer

ВОЗМОЖНОСТИ ТИПИРОВАНИЯ РАКА МОЛОЧНОЙ ЖЕЛЕЗЫ С ИСПОЛЬЗОВАНИЕМ МЕТОДИКИ ОТ-ПЦР

 Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy. Nowadays, multigene expression analysis is actively used to predict the response to chemotherapy in patients with earlystage breast cancer. We studied a 24-gene multi-gene panel for typing breast cancer.Material and Methods. A prospective analysis of 199 breast cancer patients (T1–3N0–3M0) was carried out. Surgical specimens were studied using the standard immunohistochemistry (IHC) and RT-PCR for detecting expression of 24 genes.Results. According to the IHC results, breast cancer was divided into 5 molecular subtypes: luminal A was detected in 59 (30 %) patients; luminal B (HER2-negative) in 52 (26 %); luminal B (HER2-positive) in 19 (9 %); triple-negative in 28 (14 %); HER2-positive 41 (21 %). RT-PCR showed that ST K15, MYC, MYBL2, BIRCC 5, BCL2, TERT, ESRP1, PGR, HER2, GBR7, MGB1 and MMP11 were the most significant genes in subtype distribution. The total percentage of matches between the two studies was 61.7 %.Conclusion. Studies have shown the need to add additional typing methods for breast cancer to a standard IHC study, which will undoubtedly increase the information content of diagnostic measures and will improve the effectiveness of the treatment.Введение. Адъювантная системная терапия остается одним из основных методов лечения у больных раком молочной железы. Результаты стандартного иммуногистохимического исследования не всегда в полной мере являются критерием для выбора системной терапии. Для прогнозирования эффективности лечения при ранних стадиях активно применяется мультигенный экспрессионный анализ. Была изучена отечественная мультигенная панель, состоящая из 24 генов, позволяющая типировать рак молочной железы.Материал и методы. Проводился проспективный анализ 199 больных РМЖ (T1–3N0–3M0), в ходе которого операционный материал подвергался стандартному иммуногистохимическому исследованию, а также он изучался методом ОТ-ПЦР с выявлением экспрессии 24 генов.Результаты. По результатам ИГХ осуществлялось молекулярное типирование РМЖ с выделением 5 подтипов: люминальный тип А был выявлен у 59 (30 %) больных; люминальный В (HER2-негативный) – у 52 (26 %); люминальный В (HER2-позитивный) – у 19 (9 %); трижды негативный – у 28 (14 %); HER2позитивный – у 41 (21 %) пациента. По данным ОТ-ПЦР наиболее значимыми генами в распределении на подтипы рака молочной железы являлись: ST K15, MYC, MYBL2, BIRCC 5, BCL2, TERT, ESRP1, PGR, HER2, GBR7, MGB1 и MMP11. При дальнейшем анализе суммарное число совпадений данных двух исследований составило 61,7 %.Заключение. Проведенные исследования продемонстрировали необходимость добавления дополнительных методов типирования рака молочной железы к стандартному ИГХ-исследованию, что, несомненно, повысит информативность диагностических мероприятий и позволит повысить эффективность проводимого лечения

«REFERENCE QUANTITY» OF BIODIVERSITY AS A FACTOR OF ENSURING THE STEADY ECONOMICAL-ECOLOGICAL DEVELOPMENT OF THE TERRITORY

Objective: to prove the necessity to ensure the reference level of biodiversity for the territory’s ecological development. Methods: abstract-logical, historical, trend analysis, grouping method. Results: features of preserving the Russia’ ecosystem are revealed through state natural reserves organizing. Quantity of bio-resources in Zhiguli reserve and Samara region are estimated. Scientific novelty: economic methods are formulated to ensure the reference level of biodiversity, quadro-system of economic mechanism of their implementation is designed. Practical significance: the proposed mechanism allows to ensure the steady ecological development of the territory

COURSE OF PREGNANCY AND CHILDBIRTH OF A WOMAN WITH ACHONDROPLASIA

Retrospective analysis of the favorable course of pregnancy and childbirth of a woman with congenital pathology – achondroplasia. During pregnancy clinical and immunological studies, ultrasound scans, cardiotocography were held. Pregnancy ended by giving birth to a healthy newborn by caesarean section

THE ANALYSIS OF THE EFFECTIVENESS OF THROMBOPROPHYLAXIS AMONG PREGNANT WOMEN WITH MESENCHYMAL DYSPLASIA

the analysis of the effectiveness of thromboprophylaxis among pregnant women with mesenchymal dysplasia was carried out. It was concluded that it reduces the risk of thrombohemorrhagic complications and accordingly the economic costs of medical care

ON THE WAY TO THE ECO-MODERNIZATION OF RUSSIA (REFLECTING ON THE BOOK YANICKII O.N. ECO-MODERNIZATION OF RUSSIA: THEORY, PRACTICE, PERSPECTIVES

The article discusses various aspects of eco-modernization ofRussia, presented in the monograph of Yanitsky. Objectives of environmental-ecological modernization is the change of the state environmental policy to achieve, at least, three main results: im-provement of quality of life, increase of economic efficiency and ensure a «green growth» of the economy and energy, and the conservation and restoration of the natural environment

“BRAINSTORM” OF SUSTAINABLE DEVELOPMENT INDEXES AND INDICATORS (ON THE EXAMPLE OF THE VOLGA BASIN)

Aim of the research – development of management strategies ensuring sustainable development of the territories of the Volga river basin and its administrative units.Methods. In the research we used various methods of mathematical statistics, systematic principles. We propose the conception of "brainstorming" for the assessment of the territory using indices and indicators of sustainable development.Results. We selected nine indicators and indices. We have conducted a correlation analysis of the selected indices and indicators of sustainable development, all of which reflect the state of social, ecological and economic systems. For an integrated assessment of the two objects one introduced the "critical" and the "reference" state and fixed values for each index and indicator under the review. Factor analysis in the space of two principal components is conducted as well as one carried out the calculation of generalized desirability function for each administrative unit of the Volga river basin. "Brainstorm", taking into consideration indices and indicators of sustainable development, showed similarities in the administrative units of the Volga river basin, which primarily reflects approximately the same socio-ecological-economic development, defined by a single policy and economic decisions.Conclusion. The analysis revealed different management strategies of sustainable development of regions. The first group (strategy A) includes the Republic of Tatarstan, and Chuvashia, Moscow and Samara regions, which should pay special attention to investments in improving the "life quality" by stabilizing and reducing the degree of anthropogenic load on the territory. The second group (strategy B): Astrakhan, Volgograd, Kirov, Tver and Kostroma and Perm regions - it is advisable to do finance and investment of resources in public education, increase the average lifetime, increase the incomes of the population. For the remaining areas optimally use a mixed strategy A + B for sustainable development