The relationship between dark triad personality traits and sexting behaviors among adolescents and young adults across 11 countries

Background: Sexting is an increasingly common phenomenon among adolescents and young adults. Some studies have investigated the role of personality traits in different sexting behaviors within mainstream personality taxonomies like Big Five and HEXACO. However, very few studies have investigated the role of maladaptive personality factors in sexting. Therefore, the present study investigated the relationship between Dark Triad Personality Traits and experimental (i.e., sharing own sexts), risky (i.e., sexting under substance use and with strangers), and aggravated sexting (i.e., non-consensual sexting and sexting under pressure) across 11 countries. Methods: An online survey was completed by 6093 participants (Mage = 20.35; SDage = 3.63) from 11 different countries which covered four continents (Europe, Asia, Africa, and America). Participants completed the Sexting Behaviors Questionnaire and the 12-item Dark Triad Dirty Dozen scale. Results: Hierarchical regression analyses showed that sharing own sexts was positively predicted by Machiavellianism and Narcissism. Both risky and aggravated sexting were positively predicted by Machiavellianism and Psychopathy. Conclusions: The present study provided empirical evidence that different sexting behaviors were predicted by Dark Triad Personality Traits, showing a relevant role of Machiavellian-ism in all kinds of investigated sexting behaviors. Research, clinical, and education implications for prevention programs are discussed

Способ получения рекомбинантных антител, продуцируемых клеточной линией, трансдуцированной рекомбинантными аденовирусами

Objectives. To develop a technology for obtaining recombinant antibodies in a suspension culture of human HEK293 cells using transduction with recombinant adenovirus serotype 5 (rAd5) carrying genes expressing heavy and light chains of antibodies on the example of two broadspectrum anti-influenza antibodies 27F3 and CR9114.Methods. Ad5-27F3-H, Ad5-CR9114-H, and Ad5-27F3-L recombinant adenoviruses carrying the 27F3 antibody heavy chain gene, CR9114 antibody heavy chain gene, and 27F3 light chain gene, respectively, were generated using the AdEasy™ Adenoviral vector system. To accumulate preparative amounts of recombinant r27F3 and rCR9114 antibodies, the HEK293 suspension cell line was transduced with recombinant adenoviruses carrying genes for heavy and light chains of antibodies. The cells were cultured in a wave-type bioreactor. Chromatography was used to purify recombinant antibodies from the culture medium. After analyzing the molecular weights of purified antibodies using protein electrophoresis, their ability to interact with influenza A and B viruses was analyzed using the Western blot technique, while their ability to neutralize influenza A and B viruses was evaluated using the virus neutralization assay.Results. A method for the accumulation and purification of recombinant r27F3 and CR9114 antibodies from the culture medium of a suspension culture of human cells following transduction with its recombinant adenoviruses carrying the genes for heavy and light chains of these antibodies was developed. The ability of the r27F3 antibody to interact with and neutralize influenza A viruses of group 1 (except influenza A virus subtype H2) and group 2 was shown. The ability of the rCR9114 antibody to interact with influenza A viruses of group 1 and influenza B viruses, as well as to neutralize influenza A viruses of group 1, was demonstrated.Conclusions. A technology for obtaining recombinant antibodies in a suspension culture of HEK293 cells using transduction with recombinant adenoviruses carrying genes expressing heavy and light chains of antibodies was developed along with a confirmation of their specificity.Цели. Разработать технологию получения рекомбинантных антител в суспензионной культуре клеток человека HEK293 с помощью трансдукции рекомбинантными аденовирусами человека пятого серотипа, несущими гены, экспрессирующие тяжелые и легкие цепи антител, на примере двух противогриппозных антител широкого спектра действия 27F3 и CR9114.Методы. Рекомбинантные аденовирусы Ad5-27F3-H, Ad5-CR9114-H и Ad5-27F3-L, несущие ген тяжелой цепи антитела 27F3, ген тяжелой цепи антитела CR9114 и ген легкой цепи 27F3, были получены с помощью набора AdEasy™ Adenoviral vector system. Для накопления препаративных количеств рекомбинантных антител r27F3 и rCR9114 суспензионную клеточную линию HEK293 трансдуцировали рекомбинантными аденовирусами, несущими гены тяжелых и легких цепей антител, и культивировали клетки в биореакторе волнового типа. Рекомбинантные антитела очищали из культуральной жидкости хроматографическим методом. Молекулярную массу полученных антител анализировали с помощью белкового электрофореза, их способность взаимодействовать с вирусами гриппа А и В методом вестерн-блот анализа, а способность нейтрализовать вирусы гриппа А и В с помощью реакции вирус-нейтрализации.Результаты. Отработана методика накопления и очистки рекомбинантных антител r27F3 и CR9114 из культуральной жидкости суспензионной культуры клеток человека после трансдукции ее рекомбинантными аденовирусами, несущими гены тяжелых и легких цепей этих антител. Показана способность антитела r27F3 взаимодействовать с вирусами гриппа А подгруппы 1 (кроме вируса грипп А субтипа H2) и подгруппы 2 и нейтрализовать их. Показана способность антитела rCR9114 взаимодействовать с вирусами гриппа А подгруппы 1 и вирусами гриппа В, а также нейтрализовать вирусы гриппа А подгруппы 1.Выводы. Отработана технология получения рекомбинантных антител в суспензионной культуре клеток HEK293 с помощью трансдукции рекомбинантными аденовирусами, несущими гены, экспрессирующие тяжелые и легкие цепи антител, и показана их специфичность

Processes of Redevelopment of Industrial Areas of Staint-Petersburg

The article examines an important problem of the effective use of industrial areas of St. Petersburg, which occupy significant territories in the city. The analysis of the current state of the redevelopment of industrial zones in the metropolis is given. The problems of restraining the development of investment and construction processes in this sector are identified. Conclusions are drawn about the impact of redevelopment on the life processes of residents of St. Petersburg.В статье исследована важная проблема эффективного использования промышленных зон Санкт- Петербурга, которые занимают в городе значительные по площади территории. Приведен анализ современного состояния редевелопмента промышленных зон в мегаполисе. Выявлены проблемы сдерживания развития инвестиционно-строительных процессов в этом секторе. Сделаны выводы о влиянии редевелопмента на процессы жизнедеятельности жителей Санкт-Петербурга

The role of HEXACO personality traits in different kinds of sexting: a cross-cultural study in 10 countries

Sexting has been defined as sharing sexually suggestive content (i.e., sexts) via Internet or smartphone. To date, only a few studies investigated the role of personality traits in relation to sending or receiving sexts, and most of them used the Five Factor Model of Personality. No studies applied the theoretical model of HEXACO six personality traits (i.e., honesty-humility, emotionality, extraversion, agreeableness, conscientiousness, and openness to experience) when examining different types of sexting (i.e., sending own sexts, risky sexting, sharing sexts from someone else without his/her permission, sexting under pressure). Thus, this is the first study that, using a cross-cultural perspective, investigated HEXACO personality predictors of sexting behaviors considered as a multidimensional construct. A total of 5542 participants from 13 to 30 years old (Mage = 20.36; SDage = 3.67; 60.4% girls and 39.6% boys) from 10 different countries participated in the study. Participants completed the sexting behaviors questionnaire and the HEXACO personality inventory. Four hierarchical regression analyses were conducted to investigate which HEXACO personality traits predicted different sexting behaviors, controlling for country, biological sex, age, sexual identity status, and dating relationship status. Results showed that honesty-humility and conscientiousness were negatively predictive of all investigated sexting behaviors. Emotionality and extraversion were positively related, and agreeableness was negatively related to sending own sexts and risky sexting. Finally, openness to experience was negatively related to sharing sexts from someone else without his/her consent and sexting under pressure. Results have implications for the development and implementation of sexual education and prevention programs aimed towards adolescents and young adults