Deteksi Mutasi Pada Quinolone Resistant Determining Regions (QRDRs ) Gen GyrA Pada Salmonella Typhi Isolat Klinik Dan Galur Khas Indonesia

A number of researchers in endemic regions in Asia have proved that mutations in Quinolone Resistant Determining Regions (QRDRs), parts of gyrA gene of Salmonella typhi, can reduce the susceptibility of ciprofloxacin therapeutic in enteric fever. The sites of amino acid mutations in QRDRs in relation with decreased fluoroquinolone susceptibility are different in each endemic region. The present study attempted to investigate the association of the decrease of fluoroquinolone susceptibility and the mutations in QRDRs of clinical and typical Indonesian Salmonella typhi isolates. All isolates were subjected to the antimicrobial susceptibility test applying the Kirby-Bauer method of disk diffusion with ciprofloxacin and were examined by PCR and direct nucleotide sequencing for genetic alteration in QRDRs. One ciprofloxacin sensitive isolate was observed and it showed no mutation; ciprofloxacin resistant galurs were observed in 2 isolates and they also showed no mutation. The data imply that QRDRs mutations in this research are not associated with fluoroquinolone resistance in clinical and typical Indonesian Salmonella typhi isolates. It is suggested that nucleotide re-sequencing should be used to verify this result. &nbsp

Complete characterization of quantum correlations by randomized measurements

The fact that quantum mechanics predicts stronger correlations than classical physics is an essential cornerstone of quantum information processing. Indeed, these quantum correlations are a valuable resource for various tasks, such as quantum key distribution or quantum teleportation, but characterizing these correlations in an experimental setting is a formidable task, especially in scenarios where no shared reference frames are available. By definition, quantum correlations are reference-frame independent, i.e., invariant under local transformations; this physically motivated invariance implies, however, a dedicated mathematical structure and, therefore, constitutes a roadblock for an efficient analysis of these correlations in experiments. Here we provide a method to directly measure any locally invariant property of quantum states using locally randomized measurements, and we present a detailed toolbox to analyze these correlations for two quantum bits. We implement these methods experimentally using pairs of entangled photons, characterizing their usefulness for quantum teleportation and their potential to display quantum nonlocality in its simplest form. Our results can be applied to various quantum computing platforms, allowing simple analysis of correlations between arbitrary distant qubits in the architecture.Comment: 5 pages + 15 pages of appendix, 4 figure

Stable ultrahigh-density magneto-optical recordings using introduced linear defects

The stability of data bits in magnetic recording media at ultrahigh densities is compromised by thermal `flips' -- magnetic spin reversals -- of nano-sized spin domains, which erase the stored information. Media that are magnetized perpendicular to the plane of the film, such as ultrathin cobalt films or multilayered structures, are more stable against thermal self-erasure than conventional memory devices. In this context, magneto-optical memories seem particularly promising for ultrahigh-density recording on portable disks, and bit densities of $\sim$100 Gbit inch$^{-2}$ have been demonstrated using recent advances in the bit writing and reading techniques. But the roughness and mobility of the magnetic domain walls prevents closer packing of the magnetic bits, and therefore presents a challenge to reaching even higher bit densities. Here we report that the strain imposed by a linear defect in a magnetic thin film can smooth rough domain walls over regions hundreds of micrometers in size, and halt their motion. A scaling analysis of this process, based on the generic physics of disorder-controlled elastic lines, points to a simple way by which magnetic media might be prepared that can store data at densities in excess of 1 Tbit inch$^{-2}$.Comment: 5 pages, 4 figures, see also an article in TRN News at http://www.trnmag.com/Stories/041801/Defects_boost_disc_capacity_041801.htm

A comparison of customised and prefabricated insoles to reduce risk factors for neuropathic diabetic foot ulceration: a participant-blinded randomised controlled trial.

UNLABELLED: BACKGROUND: Neuropathic diabetic foot ulceration may be prevented if the mechanical stress transmitted to the plantar tissues is reduced. Insole therapy is one practical method commonly used to reduce plantar loads and ulceration risk. The type of insole best suited to achieve this is unknown. This trial compared custom-made functional insoles with prefabricated insoles to reduce risk factors for ulceration of neuropathic diabetic feet. METHOD: A participant-blinded randomised controlled trial recruited 119 neuropathic participants with diabetes who were randomly allocated to custom-made functional or prefabricated insoles. Data were collected at issue and six month follow-up using the F-scan in-shoe pressure measurement system. Primary outcomes were: peak pressure, forefoot pressure time integral, total contact area, forefoot rate of load, duration of load as a percentage of stance. Secondary outcomes were patient perceived foot health (Bristol Foot Score), quality of life (Audit of Diabetes Dependent Quality of Life). We also assessed cost of supply and fitting. Analysis was by intention-to-treat. RESULTS: There were no differences between insoles in peak pressure, or three of the other four kinetic measures. The custom-made functional insole was slightly more effective than the prefabricated insole in reducing forefoot pressure time integral at issue (27% vs. 22%), remained more effective at six month follow-up (30% vs. 24%, p=0.001), but was more expensive (UK £656 vs. £554, p<0.001). Full compliance (minimum wear 7 hours a day 7 days per week) was reported by 40% of participants and 76% of participants reported a minimum wear of 5 hours a day 5 days per week. There was no difference in patient perception between insoles. CONCLUSION: The custom-made insoles are more expensive than prefabricated insoles evaluated in this trial and no better in reducing peak pressure. We recommend that where clinically appropriate, the more cost effective prefabricated insole should be considered for use by patients with diabetes and neuropathy. TRIAL REGISTRATION: Clinical trials.gov (NCT00999635). Note: this trial was registered on completion

Quasi-Quantum Groups, Knots, Three-Manifolds, and Topological Field Theory

We show how to construct, starting from a quasi-Hopf algebra, or quasi-quantum group, invariants of knots and links. In some cases, these invariants give rise to invariants of the three-manifolds obtained by surgery along these links. This happens for a finite-dimensional quasi-quantum group, whose definition involves a finite group $G$, and a 3-cocycle \om, which was first studied by Dijkgraaf, Pasquier and Roche. We treat this example in more detail, and argue that in this case the invariants agree with the partition function of the topological field theory of Dijkgraaf and Witten depending on the same data G, \,\om.Comment: 30 page

Pulmonary Abnormalities and Carotid Atherosclerosis in Ex-Smokers without Airflow Limitation.

Abstract It is well-established that COPD patients have a burden of vascular disease that cannot be fully-explained by smoking history but the mechanistic links between atherosclerosis and pulmonary disease in COPD patients are not well-understood. Moreover, in ex-smokers without symptoms or other evidence of COPD, subclinical pulmonary and vascular disease, although potentially present, has not been described or evaluated. Hence our aim was to use sensitive three-dimensional (3D) pulmonary and carotid imaging to quantify pulmonary airway/parenchyma abnormalities and atherosclerosis in ex-smokers without airflow limitation or symptoms consistent with COPD. We evaluated 61 subjects without airflow limitation including 34 never- (72 ± 6 years) and 27 ex-smokers (73 ± 9 years), who provided written informed consent to spirometry, plethysmography, (3)He magnetic resonance imaging (MRI) and carotid ultrasound (US) and, for ex-smokers alone, thoracic X-ray computed tomography (CT). Ex-smokers had significantly greater (3)He ventilation defect percent (VDP = 7%, p = 0.001) and carotid total plaque volume (TPV = 250 mm(3), p = 0.002) than never-smokers, although there were no significant differences for spirometry or plethysmography, and CT airway and emphysema measurements were normal. There were univariate relationships for (3)He VDP with carotid intima media thickness (IMT, r = 0.42, p = 0.004), TPV (r = 0.41, p = 0.006) and vessel wall volume (VWV, r = 0.40, p = 0.007). Multivariate models that included age, BMI, FEV1, DLCO and VDP showed that only VDP significantly predicted IMT (β = 0.41, p = 0.001), VWV (β = 0.45, p = 0.003) and TPV (β = 0.38, p = 0.005). In summary, there was imaging evidence of mild airways disease and carotid plaque burden that were related and significantly greater in ex-smokers without airflow limitation than in never-smokers

The abundance spread in the Bootes I dwarf spheroidal galaxy

We present medium-resolution spectra of 16 radial velocity red-giant members of the low-luminosity Bootes I dwarf spheroidal (dSph) galaxy, that have sufficient S/N for abundance determination, based on the strength of the Ca II K line. Assuming [Ca/Fe] ~ +0.3, the abundance range in the sample is Delta [Fe/H] ~ 1.7 dex, with one star having [Fe/H] = -3.4. The dispersion is sigma([Fe/H]) = 0.45 +/- 0.08 -- similar to those of the Galaxy's more luminous dSph systems and Omega Centauri. This suggests that the large mass (greater than approximately 10 million solar masses) normally assumed to foster self-enrichment and the production of chemical abundance spreads was provided by the non-baryonic material in Bootes I.Comment: 12 pages, 2 figures. Published in the Astrophysical Journal Letters. An erratum is in press and included here as an Appendix (none of the analysis or conclusions is affected

The JWST Resolved Stellar Populations Early Release Science Program VI. Identifying Evolved Stars in Nearby Galaxies

We present an investigation of evolved stars in the nearby star-forming galaxy WLM, using NIRCam imaging from the JWST resolved stellar populations early-release science (ERS) program. We find that various combinations of the F090W, F150W, F250M, and F430M filters can effectively isolate red supergiants (RSGs) and thermally-pulsing asymptotic giant branch (TP-AGB) stars from one another, while also providing a reasonable separation of the primary TP-AGB subtypes: carbon-rich C-type stars and oxygen-rich M-type stars. The classification scheme we present here agrees very well with the well-established Hubble Space Telescope (HST) medium-band filter technique. The ratio of C to M-type stars (C/M) is 0.8$\pm$0.1 for both the new JWST and the HST classifications, which is within one sigma of empirical predictions from optical narrow-band CN and TiO filters. The evolved star colors show good agreement with the predictions from the PARSEC$+$COLIBRI stellar evolutionary models, and the models indicate a strong metallicity dependence that makes stellar identification even more effective at higher metallicity. However, the models also indicate that evolved star identification with NIRCam may be more difficult at lower metallicies. We test every combination of NIRCam filters using the models and present additional filters that are also useful for evolved star studies. We also find that $\approx$90\% of the dusty evolved stars are carbon-rich, suggesting that carbonaceous dust dominates the present-day dust production in WLM, similar to the findings in the Magellanic Clouds. These results demonstrate the usefulness of NIRCam in identifying and classifying dust-producing stars without the need for mid-infrared data.Comment: 15 page, 12 figures, submitted to AAS Journal