Deteksi Mutasi Pada Quinolone Resistant Determining Regions (QRDRs ) Gen GyrA Pada Salmonella Typhi Isolat Klinik Dan Galur Khas Indonesia

Abstract

A number of researchers in endemic regions in Asia have proved that mutations in Quinolone Resistant Determining Regions (QRDRs), parts of gyrA gene of Salmonella typhi, can reduce the susceptibility of ciprofloxacin therapeutic in enteric fever. The sites of amino acid mutations in QRDRs in relation with decreased fluoroquinolone susceptibility are different in each endemic region. The present study attempted to investigate the association of the decrease of fluoroquinolone susceptibility and the mutations in QRDRs of clinical and typical Indonesian Salmonella typhi isolates. All isolates were subjected to the antimicrobial susceptibility test applying the Kirby-Bauer method of disk diffusion with ciprofloxacin and were examined by PCR and direct nucleotide sequencing for genetic alteration in QRDRs. One ciprofloxacin sensitive isolate was observed and it showed no mutation; ciprofloxacin resistant galurs were observed in 2 isolates and they also showed no mutation. The data imply that QRDRs mutations in this research are not associated with fluoroquinolone resistance in clinical and typical Indonesian Salmonella typhi isolates. It is suggested that nucleotide re-sequencing should be used to verify this result. &nbsp