Flight experience with a pivoting traversing boundary-layer probe

A pivoting traversing boundary layer probe was evaluated in flight on an F-104 airplane. The evaluation was performed at free stream Mach numbers from 0.8 to 2.0. The unit is described, and operating problems and their solutions are discussed. Conventional boundary layer profiles containing variations in flow angle within the viscous layer are shown for free stream Mach numbers of 0.8, 1.6, and 2.0. Although the unit was not optimized for size and weight, it successfully measured simultaneously flow angularity, probe height, and pitot pressure through the boundary layer

Design of biochemical pattern forming systems from minimal motifs

Although molecular self-organization and pattern formation are key features of life, only very few pattern-forming biochemical systems have been identified that can be reconstituted and studied in vitro under defined conditions. A systematic understanding of the underlying mechanisms is often hampered by multiple interactions, conformational flexibility and other complex features of the pattern forming proteins. Because of its compositional simplicity of only two proteins and a membrane, the MinDE system from Escherichia coli has in the past years been invaluable for deciphering the mechanisms of spatiotemporal self-organization in cells. Here, we explored the potential of reducing the complexity of this system even further, by identifying key functional motifs in the effector MinE that could be used to design pattern formation from scratch. In a combined approach of experiment and quantitative modeling, we show that starting from a minimal MinE-MinD interaction motif, pattern formation can be obtained by adding either dimerization or membrane-binding motifs. Moreover, we show that the pathways underlying pattern formation are recruitment-driven cytosolic cycling of MinE and recombination of membrane-bound MinE, and that these differ in their in vivo phenomenology

RNA secondary structure design

We consider the inverse-folding problem for RNA secondary structures: for a given (pseudo-knot-free) secondary structure find a sequence that has that structure as its ground state. If such a sequence exists, the structure is called designable. We implemented a branch-and-bound algorithm that is able to do an exhaustive search within the sequence space, i.e., gives an exact answer whether such a sequence exists. The bound required by the branch-and-bound algorithm are calculated by a dynamic programming algorithm. We consider different alphabet sizes and an ensemble of random structures, which we want to design. We find that for two letters almost none of these structures are designable. The designability improves for the three-letter case, but still a significant fraction of structures is undesignable. This changes when we look at the natural four-letter case with two pairs of complementary bases: undesignable structures are the exception, although they still exist. Finally, we also study the relation between designability and the algorithmic complexity of the branch-and-bound algorithm. Within the ensemble of structures, a high average degree of undesignability is correlated to a long time to prove that a given structure is (un-)designable. In the four-letter case, where the designability is high everywhere, the algorithmic complexity is highest in the region of naturally occurring RNA.Comment: 11 pages, 10 figure

Identifying low test-taking effort during low-stakes tests with the new Test-taking Effort Short Scale (TESS) – development and psychometrics

Background: Low-stakes tests are becoming increasingly important in international assessments of educational progress, and the validity of these results is essential especially as these results are often used for benchmarking. Test scores in these tests not only mirror students’ ability but also depend on their test-taking effort. One way to obtain more valid scores from participating samples is to identify test-takers with low test-taking effort and to exclude them from further analyses. Self-assessment is a convenient and quick way of measuring test-taking effort. We present the newly developed Test-taking Effort Short Scale (TESS), which comprises three items measuring attainment value/intrinsic value, utility value, and perceived benefits, respectively. Methods: In a multicenter validation study with N = 1837 medical students sitting a low-stakes progress test we analyzed item and test statistics including construct and external validity. Results: TESS showed very good psychometric properties. We propose an approach using stanine norms to determine a cutoff value for identifying participants with low test-taking effort. Conclusion: With just three items, TESS is shorter than most established self-assessment scales; it is thus suited for administration after low-stakes progress testing. However, further studies are necessary to establish its suitability for routine usage in assessment outside progress testing

Investigations into packaging technology for membrane-based thermal flow sensors

A new packaging method to mount a membrane-based thermal flow sensor, flush with the surface, is presented. Therefore, a specific design for the housing is shown, which is also adaptable to other conditions. It has been experimentally shown that it is important to mount the sensor flush with the surface. In addition, the experimental results are discussed. If a membrane-based thermal flow sensor is not mounted flush with the surface, vortices can occur (depending on velocity and fluid properties) or the reduction in the channel cross section plus a decrease in sensitivity have to be taken into account

Reverse Electrodialysis with saline waters and concentrated brines: a laboratory investigation towards technology scale-up

The use of concentrated brines and brackish water as feed solutions in reverse electrodialysis represents a valuable alternative to the use of river/sea water, allowing the enhancement of power output through the increase of driving force and reduction of internal stack resistance. Apart from a number of theoretical works, very few experimental investigations have been performed so far to explore this possibility. In the present work, two RED units of different size were tested using artificial saline solutions. The effects of feed concentration, temperature and flowrate on process performance parameters were analysed, adopting two different sets of membranes. These experiments allowed to identify the most favourable conditions for maximising the power output within the presently investigated range, i.e. 0.1 M NaCl as diluate and 5 M NaCl as concentrate at 40°C. Under these conditions a power density equal to 12 W/m2cell_pair was reached, among the highest so far reported in the literature. Increasing the unit size a slight reduction in power density was observed. These results indicate new directions for a successful scale-up and development of the Reverse Eletrodialysis technology

A conceptual model of the groundwater contribution to streamflow during drought in the Afon Fathew catchment, Wales

In 2022 BGS was commissioned by Dŵr Cymru Welsh Water (DCWW) to undertake desk and field investigations to develop a conceptual understanding of the contribution of groundwater to streamflow during drought in the Afon Fathew, Wales. This report details the findings of these investigations. In addition to a desk study, two field visits were completed to survey water features in the catchment, take samples for groundwater residence time indicators, and undertake a passive seismic (Tromino) geophysical survey. The results of the desk study and field visits were combined with flow accretion profile data to develop a conceptual model of groundwater flow to the Afon Fathew during drought, described herein. The Fathew is underlain by a bedrock of silty mudstones which are traditionally considered to be poor aquifers. In the Fathew catchment there is evidence from boreholes for local-scale groundwater flow in the bedrock within fractures and other discontinuities. An upper weathered layer, in combination with faulting and folding patterns, is likely to control the geometry and magnitude of bedrock groundwater flow systems and the location of springs. The residence time indicator data suggest that groundwater in the bedrock is over 40 years old. Estimated discharge from bedrock springs (< 2 l/s, 0.17 Ml/day) is very small relative to the total flow in the Fathew and tributary inflows. The Tromino has shown the superficial deposits in the catchment to be highly heterogeneous in the valley bottom. Changes in the likely permeability and areal extent of the superficial deposits going down the valley bottom correspond to changes in river flows in the Fathew based on the accretion profiles. The Fathew and its tributaries are losing over well drained alluvial gravels, and gaining over low permeability lacustrine and clay-ey alluvial deposits. The Fathew is likely to be hydraulically isolated from the Dysynni catchment. 60% of low flow inflows to the Fathew are coming directly from upland tributary inflows. where very limited superficial deposits are present. In these upland settings during dry periods it is likely that the majority of discharge is coming from baseflow from bedrock. Baseflow support to the Fathew during drought periods can be conceptualised as a two-phase system: (1) Discharge from the superficial deposits to the river, particularly associated with the down-catchment variability in the permeability and thickness of the deposits, (2) Discharge from the weathered bedrock aquifer into the river, from both springs and tributary inflows. The contribution of these two processes is likely to vary as drought conditions develop. Moreover, flows in springs and tributaries may contribute to downstream storage within the superficial deposits, which may complicate the deconvolution of the Fathew river flow hydrograph into different flow components. This temporal sequencing requires further investigation. Further work such as groundwater and surface water monitoring during dry periods and electrical resistivity tomography may be beneficial to constrain these uncertainties

Fluctuation-Facilitated Charge Migration along DNA

We propose a model Hamiltonian for charge transfer along the DNA double helix with temperature driven fluctuations in the base pair positions acting as the rate limiting factor for charge transfer between neighboring base pairs. We compare the predictions of the model with the recent work of J.K. Barton and A.H. Zewail (Proc.Natl.Acad.Sci.USA, {\bf 96}, 6014 (1999)) on the unusual two-stage charge transfer of DNA.Comment: 4 pages, 2 figure

Two-level system with a thermally fluctuating transfer matrix element: Application to the problem of DNA charge transfer

Charge transfer along the base-pair stack in DNA is modeled in terms of thermally-assisted tunneling between adjacent base pairs. Central to our approach is the notion that tunneling between fluctuating pairs is rate-limited by the requirement of their optimal alignment. We focus on this aspect of the process by modeling two adjacent base pairs in terms of a classical damped oscillator subject to thermal fluctuations as described by a Fokker-Planck equation. We find that the process is characterized by two time scales, a result that is in accord with experimental findings.Comment: original file is revtex4, 10 pages, three eps figure