50,466 research outputs found
Interaction of hemojuvelin with neogenin results in iron accumulation in human embryonic kidney 293 cells
Type 2 hereditary hemochromatosis (HH) or juvenile hemochromatosis is an early onset, genetically heterogeneous, autosomal recessive disorder of iron overload. Type 2A HH is caused by mutations in the recently cloned hemojuvelin gene (HJV; also called HFE2) (Papanikolaou, G., Samuels, M. E., Ludwig, E. H., MacDonald, M. L., Franchini, P. L., Dube, M. P., Andres, L., MacFarlane, J., Sakellaropoulos, N., Politou, M., Nemeth, E., Thompson, J., Risler, J. K., Zaborowska, C., Babakaiff, R., Radomski, C. C., Pape, T. D., Davidas, O., Christakis, J., Brissot, P., Lockitch, G., Ganz, T., Hayden, M. R., and Goldberg, Y. P. (2004) Nat. Genet. 36, 77–82), whereas Type 2B HH is caused by mutations in hepcidin. HJV is highly expressed in both skeletal muscle and liver. Mutations in HJV are implicated in the majority of diagnosed juvenile hemochromatosis patients. In this study, we stably transfected HJV cDNA into human embryonic kidney 293 cells and characterized the processing of HJV and its effect on iron homeostasis. Our results indicate that HJV is a glycosylphosphatidylinositol-linked protein and undergoes a partial autocatalytic cleavage during its intracellular processing. HJV co-immunoprecipitated with neogenin, a receptor involved in a variety of cellular signaling processes. It did not interact with the closely related receptor DCC (deleted in Colon Cancer). In addition, the HJV G320V mutant implicated in Type 2A HH did not co-immunoprecipitate with neogenin. Immunoblot analysis of ferritin levels and transferrin-55Fe accumulation studies indicated that the HJV-induced increase in intracellular iron levels in human embryonic kidney 293 cells is dependent on the presence of neogenin in the cells, thus linking these two proteins to intracellular iron homeostasis
Electronic structure and Magnetism in BaMnAs and BaMnSb
We study the properties of ThCrSi structure BaMnAs and
BaMnSb using density functional calculations of the electronic and
magnetic as well experimental measurements on single crystal samples of
BaMnAs. These materials are local moment magnets with moderate band gap
antiferromagnetic semiconducting ground states. The electronic structures show
substantial Mn - pnictogen hybridization, which stabilizes an intermediate spin
configuration for the nominally Mn. The results are discussed in the
context of possible thermoelectric applications and the relationship with the
corresponding iron / cobalt / nickel compounds Ba(Fe,Co,Ni)As
Lessons from LIMK1 enzymology and their impact on inhibitor design
LIM domain kinase 1 (LIMK1) is a key regulator of actin dynamics. It is thereby a potential therapeutic target for the prevention of fragile X syndrome and amyotrophic lateral sclerosis. Herein, we use X-ray crystallography and activity assays to describe how LIMK1 accomplishes substrate specificity, to suggest a unique ‘rock-and-poke’ mechanism of catalysis and to explore the regulation of the kinase by activation loop phosphorylation. Based on these findings, a differential scanning fluorimetry assay and a RapidFire mass spectrometry activity assay were established, leading to the discovery and confirmation of a set of small-molecule LIMK1 inhibitors. Interestingly, several of the inhibitors were inactive towards the closely related isoform LIMK2. Finally, crystal structures of the LIMK1 kinase domain in complex with inhibitors (PF-477736 and staurosporine, respectively) are presented, providing insights into LIMK1 plasticity upon inhibitor binding
Time-resolved characterization of a pulsed discharge in a stationary bubble
In recent years, plasma generation in water has been proposed for the application of water treatment. The process efficiency is believed to be improved by the introduction of bubbles in the plasma active region. For further optimization, the initiating and developmental mechanisms of plasma inside bubbles need to be understood to a greater extent. In order to meet this necessity, we investigated pulsed electrical discharge inside a stationary bubble in water. This paper deals with the evolution of the discharge and of the bubble shape during discharge, investigated by electrical characterization and fast imaging. Only several microseconds after the application of the voltage pulse, plasma light is observed. Different phases are observed during plasma formation. The plasma is strongest at the bubble surface, causing the surrounding water to evaporate. This leads to both the formation of propagating streamers into the water and the expansion and collapse of the bubble. These observations show that plasma inside a bubble has the strongest activity at the bubble surface, making it attractive for water treatment
Cluster Variation Approach to the Random-Anisotropy Blume-Emery-Griffiths Model
The random--anisotropy Blume--Emery--Griffiths model, which has been proposed
to describe the critical behavior of He--He mixtures in a porous
medium, is studied in the pair approximation of the cluster variation method
extended to disordered systems. Several new features, with respect to mean
field theory, are found, including a rich ground state, a nonzero percolation
threshold, a reentrant coexistence curve and a miscibility gap on the high
He concentration side down to zero temperature. Furthermore, nearest
neighbor correlations are introduced in the random distribution of the
anisotropy, which are shown to be responsible for the raising of the critical
temperature with respect to the pure and uncorrelated random cases and
contribute to the detachment of the coexistence curve from the line.Comment: 14 pages (plain TeX) + 12 figures (PostScript, appended), Preprint
POLFIS-TH.02/9
Superconductivity in two-band systems with variable charge carrier density. The case of MgB2
The theory of thermodynamic properties of two-band superconductor with
reduced density charge carriers is developed on the base of phonon
superconducting mechanism with strong electron-phonon interaction. This theory
is adapted to describe the behavior of critical temperature Tc, energy gaps
Delta1, Delta2, and the relative jump of electron specific heat (Cs - Cn)/Cn in
the point T = Tc along with the variation of charge carrier density in the
compound MgB2 when substitutional impurities with different valence are
introduced into the system. It is shown, that according to the filling
mechanism of energy bands which overlap on Fermi surface, the quantities Tc,
Delta1, Delta2 decrease when this compound is doped with electrons and remain
constant or weakly change when the system is doped with holes. The theory
qualitatively agrees with the experimental data. Also is shown that the
consideration of inter- and intraband scattering of electrons on impurity
potential improves this agreement.Comment: 19 pages, 6 figures, 1 table. to be published in JETP (first number
2007
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