INVESTIGATING THE FEASIBILITY OF USING MESOPOROUS SILICA PARTICLES TO DELIVER GLUCOSE DURING ANAEROBIC FERMENTATION

The study presented herein investigated a potential low-energy method to separate and concentrate glucose from a lignocellulosic hydrolysate. The motivation for this method was twofold: 1) to provide the fermentation microorganism an optimal glucose concentration and 2) to supply a fermentation media free of inhibitory compounds. Two sizes of porous silica particles (with 7 nm and 2.3 nm pores) were synthesized and their ability to adsorb glucose from solution confirmed. Next, the ability of two different microorganisms, Saccharomyces cerevisiae and Streptococcus bovis, to utilize sugars adsorbed to the porous silica particles was investigated. Both the 7 nm and 2.3 nm pore-size particles were capable of adsorbing glucose from solution. Fermentations with glucose provided adsorbed to nanoparticles were compared to fermentations with glucose dissolved in media. The success of the fermentation was assessed by using high performance liquid chromatography to compare the concentration of fermentation products at harvest time. Results indicated that although the fermentation with soluble glucose produced significantly more end-products, S. bovis demonstrated some ability to metabolize the glucose adsorbed to the 7 nm pore silica particles. No evidence was detected that S. cerevisiae could metabolize glucose adsorbed to silica particles of either pore size

Vanishing Fe 3d orbital moments in single-crystalline magnetite

We show detailed magnetic absorption spectroscopy results of an in situ cleaved high quality single crystal of magnetite. In addition the experimental setup was carefully optimized to reduce drift, self absorption, and offset phenomena as far as possible. In strong contradiction to recently published data, our observed orbital moments are nearly vanishing and the spin moments are quite close to the integer values proposed by theory. This very important issue supports the half metallic full spin polarized picture of magnetite.Comment: 7 pages, 4 figure

Deployable and erectable concepts for large spacecraft

Computerized structural sizing techniques were used to determine structural proportions of minimum mass tetrahedral truss platforms designed for low Earth and geosynchronous orbit. Optimum (minimum mass) deployable and erectable, hexagonal shaped spacecraft are sized to satisfy multiple design requirements and constraints. Strut dimensions characterizing minimum mass designs are found to be significantly more slender than those conventionally used for structural applications. Comparison studies show that mass characteristics of deployable and erectable platforms are approximately equal and that the shuttle flights required by deployable trusses become excessive above certain critical stiffness values. Recent investigations of eractable strut assembly are reviewed. Initial erectable structure assembly experiments show that a pair of astronauts can achieve EVA assembly times of 2-5 min/strut and studies indicate that an automated assembler can achieve times of less than 1 min/strut for around the clock operation

Greenhouse gas production in degrading ice-rich permafrost deposits in northeastern Siberia

Permafrost deposits have been a sink for atmospheric carbon for millennia. Thaw-erosional processes, however, can lead to rapid degradation of ice-rich permafrost and the release of substantial amounts of organic carbon (OC). The amount of the OC stored in these deposits and their potential to be microbially decomposed to the greenhouse gases carbon dioxide (CO2) and methane (CH4) depends on climatic and environmental conditions during deposition and the decomposition history before incorporation into the permafrost. Here, we examine potential greenhouse gas production in degrading ice-rich permafrost deposits from three locations in the northeast Siberian Laptev Sea region. The deposits span a period of about 55 kyr from the last glacial period and Holocene interglacial. Samples from all three locations were incubated under aerobic and anaerobic conditions for 134 days at 4 °C. Greenhouse gas production was generally higher in deposits from glacial periods, where 0.2–6.1% of the initially available OC was decomposed to CO2. In contrast, only 0.1–4.0% of initial OC were decomposed in permafrost deposits from the Holocene and the late glacial transition. Within the deposits from the Kargin interstadial period (Marine Isotope Stage 3), local depositional environments, especially soil moisture, also affected the preservation of OC. Sediments deposited under wet conditions contained more labile OC and thus produced more greenhouse gases than sediments deposited under drier conditions. To assess the greenhouse gas production potentials over longer periods, deposits from two locations were incubated for a total of 785 days. However, more than 50% of total CO2 production over 785 days occurred within the first 134 days under aerobic conditions while even 80% were produced over the same period under anaerobic conditions, which emphasizes the non-linearity of the OC decomposition processes. Methanogenesis was generally observed in active layer samples but only sporadically in permafrost samples and was several orders of magnitude smaller than CO2 production

The effect of estrogen on muscle damage biomarkers following prolonged aerobic exercise in eumenorrheic women

This study assessed the influence of estrogen (E 2 ) on muscle damage biomarkers [skeletal muscle - creatine kinase (CK); cardiac muscle - CK-MB] responses to prolonged aerobic exercise. Eumenorrheic women (n=10) who were physically active completed two 60-minute treadmill running sessions at ~60-65% maximal intensity during low E 2 (midfollicular menstrual phase) and high E 2 (midluteal menstrual phase) hormonal conditions. Blood samples were collected prior to exercise (following supine rest), immediately post-, 30 min post-, and 24 hours post-exercise to determine changes in muscle biomarkers. Resting blood samples confirmed appropriate E 2 hormonal levels Total CK concentrations increased following exercise and at 24 hours post-exercise were higher in the midfollicular low E 2 phase (p<0.001). However, CK-MB concentrations were unaffected by E 2 level or exercise (p=0.442) resulting in the ratio of CK-MB to total CK being consistently low in subject responses (i.e., indicative of skeletal muscle damage). Elevated E 2 levels reduce the CK responses of skeletal muscle, but had no effect on CK-MB responses following prolonged aerobic exercise. These findings support earlier work showing elevated E 2 is protective of skeletal muscle from exercise-induced damage associated with prolonged aerobic exercise

Structural concepts for large spacecraft

Design concepts for deployable spacecraft structures are examined with particular emphasis on mass/area optimization, stowability, and assembly, and the relations of these requirements to functional design parameters. Component and modular concepts for platforms and reflectors, structural configurations for long columns, pretension columns, and joint concepts are discussed. A brief overview of astronaut and automated assembly studies is included