Self-realization of students in work of small innovative enterprises as factor of strengthening their psychological well-being and health

The circumstances and conditions for improving the psychological health of students through their participation in the activity of small innovative enterprises established on university base are considered. The authors reveal essence of students’ self-realization in work of these enterprises, and also the main trends of students’ psychological well-being, such as: a personal involvement into entrepreneurship activity, realization of internal capacities and potential, social cooperation into scientific and innovation project

Development of the cell-ELISA test for the subtype identification of circulating influenza A(H1) and A(H3) viruses

The sensitive version of cell-ELISA was developed for the subtype-specific differentiation of current influenza A(H1N1)pdm09 and A(H3N2) viruses that are circulating in the human population. This method is based on the estimation of virus reproduction in infected MDCK cells. The detection step of this method is an interaction of the subtype-specific monoclonal antibodies (mAbs) with the viral hemagglutinin (НА) molecule. The influenza A virus strains, isolated in the 2014 epidemic season, were used to validate this method.It was shown that when using mAbs # 1/ # 2 or # 4 at a concentration of 10-15 µg/ml, the developed variant of cell-ELISA was able to detect НА protein synthesized in the infected cells of influenza A(H3N2) and A(H1N1)pdm09 viruses, respectively.The developed method can be used for the identification of modern influenza A viruses with low hemagglutination activity, which is not possible by the conventional hemagglutination inhibition test.The sensitive version of cell-ELISA was developed for the subtype-specific differentiation of current influenza A(H1N1)pdm09 and A(H3N2) viruses that are circulating in the human population. This method is based on the estimation of virus reproduction in infected MDCK cells. The detection step of this method is an interaction of the subtype-specific monoclonal antibodies (mAbs) with the viral hemagglutinin (НА) molecule. The influenza A virus strains, isolated in the 2014 epidemic season, were used to validate this method. It was shown that when using mAbs # 1/ # 2 or # 4 at a concentration of 10-15 µg/ml, the developed variant of cell-ELISA was able to detect НА protein synthesized in the infected cells of influenza A(H3N2) and A(H1N1)pdm09 viruses, respectively. The developed method can be used for the identification of modern influenza A viruses with low hemagglutination activity, which is not possible by the conventional hemagglutination inhibition test

The activated charcoal adsorption of phenol

The problems of treatment of the waste water were considered in this work. The main method of the activated charcoal adsorption of phenol was investigated. The adsorption properties of the adsorbent were also considered. Along with it Langmuir's and Friendlich's adsorption isotherm has been studied and analyzed

Influence of single amino acid substitutions in the hemagglutinin on the antigenic and receptor-binding properties of influenza virus B/Florida/04/2006 of Yamagata-like evolutionary lineage

Influenza A and B viruses use sialylated oligosaccharide chains expressed on the surface of a host cell as the cell entry receptors. The type of the bond between sialic acid (SA) and the neighboring galactose residue (Gal) is one of the main characteristics that define the type of receptor. Influenza viruses recognize SAα2-3Gal- or SAα2-6Gal-structures on the surface of the cells. Influenza A viruses of avian origin bind α2-3-sialylated glycans, while the human strains bind preferentially α2-6-sialylated ones. However, the receptor-binding specificity of influenza B viruses has not been characterized sufficiently so far. In this study, we selected the escape mutants of influenza B/Florida/04/2006 strain (Yamagata-like lineage) using monoclonal antibodies (mAb) to hemagglutinin (HA). The analysis of the amino acid sequences of mAb-induced escape mutants revealed the single amino acid substitutions 40Tyr→His, 85His→Tyr, 202Asn→Lys and 242Ser→Arg in 10F4-, 8Н11-, 8Н3- and 9А3-induced HA variants, correspondingly. It was shown that the single amino acid substitutions 202Asn→Lys and 242Ser→Arg alter the receptor-binding specificity of the influenza B virus. These findings are important for the understanding of the influence of individual amino acid residues in HA on the receptor-binding properties of influenza B Yamagata-like lineage viruses and allow us to predict the possible ways of their evolution.Influenza A and B viruses use sialylated oligosaccharide chains expressed on the surface of a host cell as the cell entry receptors. The type of the bond between sialic acid (SA) and the neighboring galactose residue (Gal) is one of the main characteristics that define the type of receptor. Influenza viruses recognize SAα2-3Gal- or SAα2-6Gal-structures on the surface of the cells. Influenza A viruses of avian origin bind α2-3-sialylated glycans, while the human strains bind preferentially α2-6-sialylated ones. However, the receptor-binding specificity of influenza B viruses has not been characterized sufficiently so far. In this study, we selected the escape mutants of influenza B/Florida/04/2006 strain (Yamagata-like lineage) using monoclonal antibodies (mAb) to hemagglutinin (HA). The analysis of the amino acid sequences of mAb-induced escape mutants revealed the single amino acid substitutions 40Tyr→His, 85His→Tyr, 202Asn→Lys and 242Ser→Arg in 10F4-, 8Н11-, 8Н3- and 9А3-induced HA variants, correspondingly. It was shown that the single amino acid substitutions 202Asn→Lys and 242Ser→Arg alter the receptor-binding specificity of the influenza B virus. These findings are important for the understanding of the influence of individual amino acid residues in HA on the receptor-binding properties of influenza B Yamagata-like lineage viruses and allow us to predict the possible ways of their evolution

A rare case of acute hematogenic metadiaphyseal osteomyelitis of a newborn

The purpose of the study is to describe a case of metadiaphyseal osteomyelitis in a newborn.Цель исследования – Описание случая метадиафизарного остеомиелита новорожденного

Self-realization of students as the value of small business enterprises development in russian universities

The article deals with the psychological resources of the activity of small innovative enterprises at the university, connected with ensuring students' selfrealization. The study identifies the role and value of self-realization of students in the activities of these enterprises, and also the key conditions for selfrealization, such as: personal immersion in innovative business, attracting students' abilities and potential, social affinity in the process of entrepreneurial wor

StopCOVID cohort : An observational study of 3,480 patients admitted to the Sechenov University hospital network in Moscow city for suspected COVID-19 infection

© 2020 Oxford University Press. This is a pre-copyedited, author-produced PDF of an article accepted for publication in Clinical Infectious Diseases following peer review. The version of record is available online at: https://doi.org/10.1093/cid/ciaa1535.BACKGROUND: The epidemiology, clinical course, and outcomes of COVID-19 patients in the Russian population are unknown. Information on the differences between laboratory-confirmed and clinically-diagnosed COVID-19 in real-life settings is lacking. METHODS: We extracted data from the medical records of adult patients who were consecutively admitted for suspected COVID-19 infection in Moscow, between April 8 and May 28, 2020. RESULTS: Of the 4261 patients hospitalised for suspected COVID-19, outcomes were available for 3480 patients (median age 56 years (interquartile range 45-66). The commonest comorbidities were hypertension, obesity, chronic cardiac disease and diabetes. Half of the patients (n=1728) had a positive RT-PCR while 1748 were negative on RT-PCR but had clinical symptoms and characteristic CT signs suggestive of COVID-19 infection.No significant differences in frequency of symptoms, laboratory test results and risk factors for in-hospital mortality were found between those exclusively clinically diagnosed or with positive SARS-CoV-2 RT-PCR.In a multivariable logistic regression model the following were associated with in-hospital mortality; older age (per 1 year increase) odds ratio [OR] 1.05 (95% confidence interval (CI) 1.03 - 1.06); male sex (OR 1.71, 1.24 - 2.37); chronic kidney disease (OR 2.99, 1.89 - 4.64); diabetes (OR 2.1, 1.46 - 2.99); chronic cardiac disease (OR 1.78, 1.24 - 2.57) and dementia (OR 2.73, 1.34 - 5.47). CONCLUSIONS: Age, male sex, and chronic comorbidities were risk factors for in-hospital mortality. The combination of clinical features were sufficient to diagnoseCOVID-19 infection indicating that laboratory testing is not critical in real-life clinical practice.Peer reviewe

INTRODUCTION OF ELECTRIC HEATING IN MULTI-APARTMENT BUILDINGS – AN ALTERNATIVE TO CENTRALIZED HEATING

На Урале накоплен уникальный опыт электрического отопления многоэтажных жилых зданий. Построено более 10 многоэтажных зданий с электроотоплением в городах Екатеринбурге и Челябинске. Получены надежные статистические данные по эксплуатационным затратам от управляющих компаний, т. к. некоторые дома эксплуатируются более 10 лет.In the Urals, a unique experience has been accumulated in electric heating of multi-story residential buildings. More than 10 multi-story buildings with electric heating were built in Yekaterinburg and Chelyabinsk. There is an operational experience of more than 10 years

Ингаляционный оксид азота при первичной легочной гипертензии: влияние на легочную гемодинамику, газообмен и транспорт кислорода

The study assess effects of inhaled nitric oxide on pulmonary haemodynamics, gas exchange and oxygen transport in patients with primary pulmonary hypertension (PPH) and evaluates factors predicting the response to inhaled nitric oxide.Ten patients with PPH (8 females, 2 males) were observed.Oxygenation and haemodynamic variables were measures and calculated at room air and then 15 min after each sequential addition of 10, 20 and 40 ppm nitric oxide to the gas mixture. Patients were considered responders if the mean pulmonary artery pressure or pulmonary vascular resistance decreased by 20%. An electrochemical gas sensor device PrinterNOx provided continuous analysis of nitric oxide and oxidative nitric oxide products.There was a dose-dependent improvement in haemodynamic variables that was maximal at 40 ppm nitric oxide (mean pulmonary artery pressure decreased from 67±7 to 56±5 mm Hg, pulmonary vascular resistance index decreased from 1335±302 to 790±102 dyne sec/cm-5, and cardiac index increased from 2.3 to 2.7 L/min/m2, all p<0.05). There was a significant improvement in oxygen transport at 20 and 40 ppm nitric oxide (DO2 improved from 684±108 to 791±110 and 835±98, respectively, p<0.05). Five patients (50%) responded to NO. The responders differed from non-responders in such factors as distance during 6-minute walking test, pa02 and paC02 (all p≤0.01).Our data show an improvement in pulmonary haemodynamics and oxygen transport in patients with primary pulmonary hypertension.В статье изучены эффекты ингаляционного оксида азота (N0) на легочную гемодинамику, газообмен и транспорт кислорода у больных с первичной легочной гипертензией (ПЛГ) и факторы-предикторы ответа на N0. Наблюдали 10 больных (8 женщин, 2 мужчин) с ПЛГ.Параметры гемодинамики и оксигенации измерялись до начала исследования и последовательно в конце сеансов ингаляции N0 в концентрациях 10, 20, и 40 ppm (через 15 мин). Пациенты считались “ответчиками” при наличии снижения среднего давления в легочной артерии или легочного сосудистого сопротивления более чем на 20%. Для постоянного контроля N0 и N02 был использован электрохимический анализатор PrinterNOx.На фоне терапии N0 наблюдалось дозозависимое улучшение легочной гемодинамики, максимальный эффект отмечен при N0 40 ppm (среднее легочное давление снизилось с 67±7 до 56±5 л/мин/м2, легочное сосудистое сопротивление – с 335±302 до 790±102 дин-с/см-5 и сердечный индекс увеличился с 2,3 до 2,7 L/min/m2, р<0,05). При N0 20 и 40 ppm наблюдалось значимое улучшение транспорта кислорода (от 684±108 до 791 ± 110 и 835±98 соответственно, р< 0,05). Пять больных (50%) ответили на терапию N0. “Ответчики” отличались от "неответчиков" по таким показателям, как дистанция во время теста с 6-минутной ходьбой, ра02 и раС02 (р<0,01).Ингаляционный оксид азота достоверно улучшает показатели легочной гемодинамики и газообмена у больных с ПЛГ