Partial persistence of exercise-induced myocardial angiogenesis following 4-week detraining in the rat

Enhanced angiogenesis, or capillary growth, has a prominent role among the various beneficial effects of exercise training on the myocardium. The aim of the present study is to assess if training-induced increases in capillarity and vascularization persist after 4 weeks of detraining. Adult male rats were trained to run on a treadmill for 10 weeks at approximately 60% VO(2max), which did not induce cardiac hypertrophy, but increased (P < 0.05) the soleus/body weight ratio, left ventricle capillarity and von Willebrand-positive cell density (n = 6). In another group of animals (n = 6) subjected to training followed by 4-week detraining, the soleus/body weight ratio returned to normal, with only partial reversal of left ventricle capillarity and von Willebrand-positive cell density. Markers of angiogenesis (VEGF, KDR/VEGF-R2 and HIF-1alpha mRNA, studied by real-time RT-PCR) were upregulated at the end of training, and returned to baseline value after detraining. Electron microscopy highlighted some morphological features in trained hearts (endothelial cell sprouting and bridges and pericyte detachment), suggestive of endothelial cell proliferation and capillary growth that were absent in untrained and detrained hearts. We conclude that the training-induced increase in cardiac capillarity and vascularization are retained for some time upon cessation of the training program even in the absence of angiogenic stimuli

Inside the "People of the Wheelbarrows": participation between online and offline dimension in the post-quake social movement

The aim of this paper is to analyze a social movement which emerged after the earthquake in L'Aquila (Italy): "The People of the Wheelbarrows". With triangulation of semi-structured interviews, offline ethnography and content analysis of the movement's Facebook group we tried to answer to the following research question: how is the movement's participation articulated between online and the offline dimensions? Our findings show the importance of the local level, the continuous interplay between the online and the offline and the simultaneous use of a variety of online platforms. The results highlight the role that the Web has played in the coordination of the movement and in the management of meetings and events and show that when physical spaces collapse, online spaces acquire a vital importance

Anatomy of the Italian Web TV ecosystem.

The aim of this article is to provide an overview of the emergent Italian Web TV ecosystem. We begin by sketching a summary of the Italian media scenario, focusing on three related aspects: the Rai-Mediaset duopoly, the Berlusconi anomaly and digital evolution of the TV system. We then switch to the Italian digital resistance scenario and describe some of the most interesting experiences developed in the Italian context. In the third part, we dissect and analyze the phenomenon of Italian Web TVs, exploring its roots, legal status, producers and audiences. We conclude by providing a reflection on Italian Web TVs as an ecosystem, both by pointing out some future challenges it will face within the Italian media scenario and by focusing on the role of active citizens and unprofessional producers in changing the scenario and in advocating pluralism and creativeness

Nucleolar size and activity are related to pRb and p53 status in human breast cancer

Cell proliferation is tightly coordinated with cell growth. The oncosuppressor proteins pRb and p53 may exert a key role in coupling growth and proliferation by controlling both ribosome biogenesis and cell cycle progression. In the present study we evaluated the relationship between the pRb and p53 status and rRNA transcriptional activity in histological sections of 343 human primary breast carcinomas. Ribosomal biogenesis was quantified by morphometric analysis of silver-stained interphase nucleolar organizer regions (AgNORs). pRb and p53 status was assessed by immunohistochemistry. Twenty-four tumors were considered to be pRb deleted, 260 tumors showed a phosphorylated-pRb labeling index (LI) up to 25%, and 55 tumors an LI >25%. Tumors with deleted pRb or phosphorylated-pRb-LI > or =25% were characterized by significantly greater mean AgNOR area values than those with unaltered pRb (p&lt;0.001). In the 71 tumors with mutated p53 the NOR area mean value was greater than in the 272 tumors with normal p53 (p&lt;0.001). Our results demonstrate, for the first time in vivo, that pRb and p53 status is related to the ribosome biogenesis rate and suggest that in tumors with altered pRb and p53 function the up-regulation of rRNA synthesis may always assure an adequate growth to cancer cells with uncontrolled cell cycle progression