Purification, crystallization and preliminary X-ray analysis of the lytic transglycosylase MltA from Escherichia coli

The lytic transglycosylase MltA from Escherichia coli with its membrane anchor and signal sequence deleted has been purified to homogeneity by means of cation-exchange chromatography. The enzyme was crystallized using the hanging-drop vapour-diffusion method. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a=b=103.70, c=109.84 Angstrom and one molecule per asymmetric unit. Crystals diffract to 2.2 Angstrom resolution on a synchrotron-radiation source

Vervaardiging en nauwkeurigheid van het LGN2 - grondgebruiksbestand : achtergrondinformatie bij gebruik van het bestand

De ontwikkelingen op het gebied van milieu, waterbeheer en ruimtelijke ordening vragen om actuele gegevens over het grondgebruik. Het tweede landelijke grondgebruiksbestand van Nederland (LGN2) is vervaardigd uit satellietbeelden van 1990, 1992 en 1994 gecombineerd met het Basisbestand Ruimtelijke Structuren (BARS) van de Rijksplanologische Dienst, ondersteund met topografische kaarten, luchtfoto's, landbouwstatistieken van het Centraal Bureau voor de Statistiek en referentiegegevens uit het veld. Deinformatie is opgeslagen in rastervorm met cellen van 25 m x 25 m. De nauwkeurigheid en betrouwbaarheid van de 5 hoofdklassen is 90 en van de 25 subklassen 70-90

Welk gras Is groener? Persoonlijke verhalen van gepromoveerden over hun loopbanen binnen en buiten de wetenschap

Merit, Expertise and Measuremen

Which grass is greener? Personal stories from PhDs about their careers within and outside academia

Merit, Expertise and Measuremen

Diagnostic procedures for non-small-cell lung cancer (NSCLC): recommendations of the European Expert Group

Background There is currently no Europe-wide consensus on the appropriate preanalytical measures and workflow to optimise procedures for tissue-based molecular testing of non-small-cell lung cancer (NSCLC). To address this, a group of lung cancer experts (see list of authors) convened to discuss and propose standard operating procedures (SOPs) for NSCLC. Methods Based on earlier meetings and scientific expertise on lung cancer, a multidisciplinary group meeting was aligned. The aim was to include all relevant aspects concerning NSCLC diagnosis. After careful consideration, the following topics were selected and each was reviewed by the experts: surgical resection and sampling; biopsy procedures for analysis; preanalytical and other variables affecting quality of tissue; tissue conservation; testing procedures for epidermal growth factor receptor, anaplastic lymphoma kinase and ROS proto-oncogene 1, receptor tyrosine kinase (ROS1) in lung tissue and cytological specimens; as well as standardised reporting and quality control (QC). Finally, an optimal workflow was described. Results Suggested optimal procedures and workflows are discussed in detail. The broad consensus was that the complex workflow presented can only be executed effectively by an interdisciplinary approach using a well-trained team. Conclusions To optimise diagnosis and treatment of patients with NSCLC, it is essential to establish SOPs that are adaptable to the local situation. In addition, a continuous QC system and a local multidisciplinary tumour-type-oriented board are essential