Rapid micropropagation of cocoyam (Xanthosoma sagittifolium) Schott

1993 Spring.Covers not scanned.Includes bibliographical references.Cocoyam (Xanthosoma sagittifolium Schott) was rapidly micropropagated through early subcultures at four and six week intervals and division of the microshoots. Shoot tips of approximately 3-5mm in size were used in the initiation of cultures on a modified B5 basal salt medium for six weeks. Cultures were subsequently micro propagated successfully through several procedures, which included the use of a range of growth regulator levels. Levels of 0.05μM NAA with 5, 10 and 20μM BAP as well as 1, 2 and 4μM TDZ singly or in combinations at the initiation and multiplication stages, as well as on agitated and stationary liquid media. Cultures initiation on solid media or supported on filter paper bridges was unsuccessful. However, solid media were beneficial in shoot multiplication, elongation and rooting stages. The level of 2μM TDZ alone and in combination with 20μM BAP significantly enhanced shoot proliferation, producing an average of 30 microshoots/culture, but repressed root formation. However, root initiation and development was possible in media containing only BAP at all levels tested. Shoot proliferation, elongation and rooting continued on media devoid of plant growth regulators and was independent of the culture vessel employed. A 100% survival of plantlets transferred into the greenhouse was achieved, irrespective of the method of acclimatization and size of the plantlet. Plantlets acclimatized in the humidity tent were less stressed, produced more and shed fewer leaves after two weeks from acclimatization. Differences in level of leaf injury were evident with control plants transferred directly to the open bench, which sustained the highest injury. The high level of survival of plantlets upon transfer to the greenhouse was attributed, in part, to the reduced numbers of stomates found on both abaxial and adaxial leaf surfaces of in vitro cultured plantlets, the high wax content and high rate of rooting

An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium (L) Schott]

Sprouted corm sections of “South Dade” white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3–5 mm comprising the apical meristem with 4–6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg's B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA) were used throughout the study. Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used. Erlenmeyer flasks and test tubes were used for growing cultures. The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested. Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures. All concentrations tested (5–20 μM BAP and 1–4 μM TDZ) produced more axillary shoots per shoot tip than the control without cytokinins. Greater proliferation rates were obtained through the use of 20 μM BAP and 2 μM TDZ, respectively, 12 weeks from initiation. Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted. The use of stationary liquid media is recommended for economic reasons

Growth of wildtype and mutant E. coli strains in minimal media for optimal production of nucleic acids for preparing labeled nucleotides

Since RNAs lie at the center of most cellular processes, there is a need for synthesizing large amounts of RNAs made from stable isotope-labeled nucleotides to advance the study of their structure and dynamics by nuclear magnetic resonance (NMR) spectroscopy. A particularly effective means of obtaining labeled nucleotides is to harvest these nucleotides from bacteria grown in defined minimal media supplemented with 15NH4Cl and various carbon sources. Given the high cost of carbon precursors required for labeling nucleic acids for NMR studies, it becomes important to evaluate the optimal growth for commonly used strains under standard minimal media conditions. Such information is lacking. In this study, we characterize the growth for Escherichia coli strains K12, K10zwf, and DL323 in three minimal media with isotopic-labeled carbon sources of acetate, glycerol, and glycerol combined with formate. Of the three media, the LeMaster-Richards and the Studier media outperform the commonly used M9 media and both support optimal growth of E. coli for the production of nucleotides. However, the growth of all three E. coli strains in acetate is reduced almost twofold compared to growth in glycerol. Analysis of the metabolic pathway and previous gene array studies help to explain this differential growth in glycerol and acetate. These studies should benefit efforts to make selective 13C-15N isotopic-labeled nucleotides for synthesizing biologically important RNAs

A Major Ingredient of Green Tea Rescues Mice from Lethal Sepsis Partly by Inhibiting HMGB1

Background. The pathogenesis of sepsis is mediated in part by bacterial endotoxin, which stimulates macrophages/ monocytes to sequentially release early (e.g., TNF, IL-1, and IFN-c) and late (e.g., HMGB1) pro-inflammatory cytokines. Our recent discovery of HMGB1 as a late mediator of lethal sepsis has prompted investigation for development of new experimental therapeutics. We previously reported that green tea brewed from the leaves of the plant Camellia sinensis is effective in inhibiting endotoxin-induced HMGB1 release. Methods and Findings. Here we demonstrate that its major component, (-)-epigallocatechin-3-gallate (EGCG), but not catechin or ethyl gallate, dose-dependently abrogated HMGB1 release in macrophage/monocyte cultures, even when given 2–6 hours post LPS stimulation. Intraperitoneal administration of EGCG protected mice against lethal endotoxemia, and rescued mice from lethal sepsis even when the first dose was given 24 hours after cecal ligation and puncture. The therapeutic effects were partly attributable to: 1) attenuation of systemic accumulation of proinflammatory mediator (e.g., HMGB1) and surrogate marker (e.g., IL-6 and KC) of lethal sepsis; and 2) suppression of HMGB1-mediated inflammatory responses by preventing clustering of exogenous HMGB1 on macrophage cell surface. Conclusions. Taken together, these data suggest a novel mechanism by which the major green tea component, EGCG, protects against lethal endotoxemia and sepsis

Global linear stability analysis of kinetic Trapped Ion Mode (TIM) turbulence in tokamak plasma using spectral method

Trapped ion modes (TIM) which belong to the family of ion temperature gradient (ITG) modes, is one of the important ingredients in heat turbulent transport at the ion scale in tokamak plasmas. It is essential to properly estimate their linear growth rate to understand their influence on ion-scale turbulent transport. A global linear analysis of a reduced gyro-bounce kinetic model for trapped particle modes is performed, and a spectral method is proposed to solve the dispersion relation. Importantly, the radial profile of the particle drift velocity is taken into account in the linear analysis by considering the exact magnetic flux {\psi} dependency of the equilibrium Hamiltonian H_{eq}({\psi}) in the quasi-neutrality equation and equilibrium gyro-bounce averaged distribution function F_{eq} . Using this spectral method, linear growth-rates of TIM instability in presence of different temperature profiles and precession frequencies of trapped ions, with an approximated constant Hamiltonian and the exact {\psi} dependent equilibrium Hamiltonian, are investigated. The growth-rate depends on the logarithmic gradient of temperature \kappa_{T} , density \kappa_{n} and equilibrium Hamiltonian \kappa_{\Lambda} . With the exact {\psi} dependent Hamiltonian, the growth rates and potential profiles are modified significantly, compared to the cases with approximated constant Hamiltonian. All the results from the global linear analysis agree with a semi-Lagrangian based linear Vlasov solver with a good accuracy. This spectral method is very fast and requires very less computation resources compared to a linear version of Vlasov-solver based on a semi-Lagrangian scheme

Therapeutic potential of HMGB1-targeting agents in sepsis

Sepsis refers to a systemic inflammatory response syndrome resulting from a microbial infection. The inflammatory response is partly mediated by innate immune cells (such as macrophages, monocytes and neutrophils), which not only ingest and eliminate invading pathogens but also initiate an inflammatory response upon recognition of pathogen-associated molecular patterns (PAMPs). The prevailing theories of sepsis as a dysregulated inflammatory response, as manifested by excessive release of inflammatory mediators such as tumour necrosis factor and high-mobility group box 1 protein (HMGB1), are supported by extensive studies employing animal models of sepsis. Here we review emerging evidence that support extracellular HMGB1 as a late mediator of experimental sepsis, and discuss the therapeutic potential of several HMGB1-targeting agents (including neutralising antibodies and steroid-like tanshinones) in experimental sepsis