The effects of Kaempferia parviflora on anti-internalization activity of Helicobacter pylori to HEp-2 cells

Helicobacter pylori, an etiological agent of active chronic gastritis and peptic ulcer disease, is now considered to be an invasive enteropathogen. Anti-adhesion and anti-internalization are new strategies for prevention and treatment of bacterial infection including the alternative of medicinal plants. In this study, four parts of Kaempferia parviflora’s extracts composing of volatile oil, hexane, ethyl acetate and methanol were examined for their antibacterial and anti-internalization activities of H. pylori against HEp-2 cells. All extracts except volatile oil showed significant antibacterial activity and had a minimum inhibitory concentration (MIC) ranging from 32 - 64 ìg/ml. The most active extract of ethyl acetateexhibited significant anti-internalization activity which corresponded to dose and time of treatment. Moreover, K. parviflora’s ethyl acetate extract could significantly inhibit the invasion of both H. pylori virulent strains (cagA+) and non-virulent strains (cagA-) in HEp-2 cells. Thus, K. parviflora is one of the effective herbs for potential prevention and treatment of H. pylori infection

Evaluation of the Antidiabetic and Antibacterial Activity of Cissus sicyoides

In this work we investigated the antidiabetic and antibacterial effect of Cissus sicyoides (CS) from Brazil. Diabetic rats that received water (A group) or extracts from the aerial parts of the plant (Cs group) during four weeks were employed. After this period, serum levels of glucose, cholesterol and triglycerides were measured. Glycemia was not affected by treatment with CS. However, there was an increased cholesterol and triglyceride level in Cs group. In addition, bioassay-guided fractionation of methanolic extract from aerial parts of CS was performed for isolation of antibacterial compounds.beta-Sitosterol and sitosterol-beta-D-glucopyranoside isolated showed antibacterial activity against Bacillus subtilis with minimal inhibitory concentrations (MICs) of 50 mug/ml and 100 mug/ml, respectively. In spite of popular belief, CS did not show antidiabetic activity. However, two compounds isolated from aerial parts of the plant (beta-sitosterol and sitosterol-beta-D-glucopyranoside) showed antibacterial activity.<br>No presente trabalho foram investigados os efeitos antibacteriano e antidiabético da planta Cissus sicyoides (CS) coletada no Brasil. Ratos diabéticos receberam água (grupo A) ou extratos da parte aérea da planta (grupo CS) durante 4 semanas. Após este período, os níveis séricos de glicose, colesterol e triglicerídeos dos ratos foram determinados. A glicemia não foi afetada pelo tratamento com CS. Entretanto, houve aumento nos níveis de colesterol e triglicerídeos nos ratos do grupo CS. Em adição, fracionamento bio-monitorado foi realizado para o isolamento de compostos com atividade antibacteriana. beta-Sitosterol e sitosterol-beta-D-glucopiranosídeo isolados mostram atividade antibacteriana contra Bacillus subtilis com concentrações mínimas inibitórias (MICs) de 50 mig/ml e 100 mig/ml, respectivamente. Apesar da crença popular, CS não mostrou atividade antidiabética. Entretanto, dois compostos isolados da parte aérea da planta (beta-sitosterol e sitosterol-beta-D-glucopiranosídeo) apresentaram fraca atividade antibacteriana