The proinflammatore cytokine production by peripheral blood cells in children with juvenile idiopathic arthritis

Children and teenagers with juvenile idiopathic arthritis (n=100) and conditionally healthy children (n=31) were investigated. The concentration of IL-1β, IL-6, IL-8, IF-γ и TNF-α in supernatants of spontaneous and stimulated by phitogemmagglutinin peripheral blood cells cultures were defined by ELISA. It was found that the spontaneous production of IL-1β, IL-8, IF-γ и TNF-α was more intensive in patients with arthritis in comparison with control, and it was the evidence of important role of these cytokines in pathogenesis of juvenile arthritis. Low mitogenstimulated levels of IL-1β, IL-8 and IF-γ in children with arthritis in comparison with healthy children indicates depletion of immunocompetent cells functional reserves.Обследованы дети и подростки 2—17 лет с ювенильным идиопатическим артритом (n=100) и условно здоровые дети соответствующего возраста (контроль, n=31). Методом ИФА определяли концентрацию ИЛ-1β, ИЛ-6, ИЛ-8, ИНФ-γ и ФНО-α в супернатантах спонтанных и стимулированных фитогемагглютинином культур клеток периферической крови. Выявлена более интенсивная спонтанная продукция клетками крови ИЛ-1β, ИЛ-8, ФНОα и ИНФγ у больных по сравнению с контрольной группой, что отражает участие данных цитокинов в патогенезе ювенильного артрита. Снижение выработки ИЛ-1β, ИЛ-8 и ИНФγ при стимуляции митогеном у детей с ювенильным идиопатическим артритом по сравнению со здоровыми детьми свидетельствует об истощении функциональных резервов иммунокомпетентных клеток

Myeloablative conditioning for allo-HSCT in pediatric ALL: FTBI or chemotherapy?—A multicenter EBMT-PDWP study

Although most children with acute lymphoblastic leukemia (ALL) receive fractionated total body irradiation (FTBI) as myeloablative conditioning (MAC) for allogeneic hematopoietic stem cell transplantation (allo-HSCT), it is an important matter of debate if chemotherapy can effectively replace FTBI. To compare outcomes after FTBI versus chemotherapy-based conditioning (CC), we performed a retrospective EBMT registry study. Children aged 2-18 years after MAC for first allo-HSCT of bone marrow (BM) or peripheral blood stem cells (PBSC) from matched-related (MRD) or unrelated donors (UD) in first (CR1) or second remission (CR2) between 2000 and 2012 were included. Propensity score weighting was used to control pretreatment imbalances of the observed variables. 3.054 patients were analyzed. CR1 (1.498): median follow-up (FU) after FTBI (1.285) and CC (213) was 6.8 and 6.1 years. Survivals were not significantly different. CR2 (1.556): median FU after FTBI (1.345) and CC (211) was 6.2 years. Outcomes after FTBI were superior as compared with CC with regard to overall survival (OS), leukemia-free survival (LFS), relapse incidence (RI), and nonrelapse mortality (NRM). However, we must emphasize the preliminary character of the results of this retrospective "real-world-practice" study. These findings will be prospectively assessed in the ALL SCTped 2012 FORUM trial.Transplantation and immunomodulatio

Fragmentation and Multifragmentation of 10.6A GeV Gold Nuclei

We present the results of a study performed on the interactions of 10.6A GeV gold nuclei in nuclear emulsions. In a minimum bias sample of 1311 interac- tions, 5260 helium nuclei and 2622 heavy fragments were observed as Au projec- tile fragments. The experimental data are analyzed with particular emphasis of target separation interactions in emulsions and study of criticalexponents. Multiplicity distributions of the fast-moving projectile fragments are inves- tigated. Charged fragment moments, conditional moments as well as two and three -body asymmetries of the fast moving projectile particles are determined in terms of the total charge remaining bound in the multiply charged projectile fragments. Some differences in the average yields of helium nuclei and heavier fragments are observed, which may be attributed to a target effect. However, two and three-body asymmetries and conditional moments indicate that the breakup mechanism of the projectile seems to be independent of target mass. We looked for evidence of critical point observable in finite nuclei by study the resulting charged fragments distributions. We have obtained the values for the critical exponents gamma, beta and tau and compare our results with those at lower energy experiment (1.0A GeV data). The values suggest that a phase transition like behavior, is observed.Comment: latex, revtex, 28 pages, 12 figures, 3tables, submitted to Europysics Journal

DIABETIChESKAYa RETINOPATIYa ? PRIChINA SLEPOTY I INVALIDNOSTI

Цель. Изучение клинико-эпидемиологических особенностей инвалидности по зрению вследствие СД в г.Москве в течение 1994-2000 гг. Материалы и методы. Объектом исследования явились лица впервые (ВПИ) и повторно (ППИ) признанные инвалидами по зрению вследствие офтальмологических осложнений (00) СД в указанный период времени. Методом сплошного наблюдения проведена выкопировка актов освидетельствования этих лиц в специализированных офтальмологических бюро МСЭ Москвы. Методом интервью был проведен опрос освидетельствуемых лиц. Объем наблюдений составил 515 лиц ВПИ и 1462 лица ППИ. Результаты. Распространенность первичной инвалидности по зрению вследствие СД в популяции зарегистрированных больных СД составила в среднем (в 1994 ?2000 гг.) в Москве 47,6 на 100 тыс. больных. В 97% случаев лицам ВПИ по зрению при СД были установлены 1-я и 2-я группа инвалидности. Это свидетельствует о быстрой и тяжелой инвалидизации лиц с ОО СД. 27,2% лиц при первичном освидетельствовании и признании их инвалидами в связи с НО СД не были диагностированы ОО СД, которые давали основания для установления более тяжелой группы инвалидности. В 26,0% случаев была установлена 3-я группа инвалидности по соматическому заболеванию, а по состоянию органа зрения можно было установить 2-ю группу. В 1,2% случаев была установлена 2-я группа инвалидности по соматическому заболеванию, а по состоянию органа зрения могла быть установлена 1-я группа. Выводы. Показатели распространенности первичной инвалидности по зрению при СД в Москве на протяжении 1994-2000 гг. имеют тенденцию к снижению. Среди лиц ВПИ вследствие ОО СД больных трудоспособного возраста составляют 1/3. При первичном освидетельствовании больны: с ОО СД в 97% случаев больным устанавливание 1-я и 2-я группы инвалидности, что свидетельствует о тяжелой первичной инвалидизации. В настоящее время в Москве существует невыявленная первичная инвалидность по зрению при СД. При медико-социальной экспертизе больных НО СД следует учитывать офтальмологический статус этих лиц и проводить офтальмологическую МСЭ

Nutritional status and tissue composition in children after hematopoietic stem cell transplantation

Hematopoietic stem cells transplantation (HSCT) is currently widely used for solid tumors, leukemia and autoimmune diseases therapy. Complications in post-transplant period, as well as specific therapy of these complications lead to nutritional status changes, which worsen post-transplant period and influence of outcome. To analyze nutritional status and tissue composition characteristics and determine the value of bioimpedance methods for complex examination of nutritional status in children after HSCT are the goal of this study. Changes in nutritional status and tissue composition of 34 children during HSCT due to various malignant and non-malignant diseases were analyzed. Bioimpedance measurements and somatometry was used to assess tissue composition. Significant worsening of nutritional status, skeletal muscle mass loss with a relatively stable adipose tissue to day +100 has been shown. A significant decrease of active cell mass and phase angle in all study phases were revealed. A high correlation between body fat mass index and skin-fat folds size over the triceps (r = 0.86), as well as between skeletal muscle mass index and shoulder muscles circle size (r = 0.82) was revealed. It is concluded that in the early post-transplant period (up to 100 days) nutritional status significantly worsened and tissue imbalance developed with a relative predominance of fat component and somatic protein pool reduction. Importance of comprehensive evaluation and monitoring of nutritional status to develop a strategy for nutritional support in children after HSCT is discussed.</p

LYMPHOID NEOPLASMS AFTER ALLOGENEIC HEMATOPOIETIC STEM CELLS TRANSPLANTATION. THE CASE OF SECONDARY HODGKIN’S LYMPHOMA, DIFFERENTIAL DIAGNOSIS WITH POST-TRANSPLANT LYMPHOPROLIFERATIVE DISEASE

Secondary malignant diseases are dangerous long-term complications of allogeneic hematopoietic stem cell transplantation (HSCT). This article describes the group of secondary lymphoid neoplasms and presents a rare case of secondary Hodgkin disease in a 21-year patient 6 years after HSCT due to acute myeloid leukemia. This complication should be distinguished from the Hodgkin-like posttransplant lymphoproliferative disease, because of the different therapy approaches and outcomes. We describe their main features and differential diagnostic procedures

Biological Characteristics of Mesenchymal Stem Cells during Ex Vivo Expansion

Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The objective of this study was to examine the kinetics of growth (MSC from 4-3 passages had significantly higher proliferative activity compared with cultures of 10-12 passages), changes in immunophenotypic characteristics of human MSC in early (3-4) and late (10-12) passages when cultured in vitro as well as study of genetic stability. In the analyzed cultures of MSC was found normal karyotype (46, XY or 46, XX). In most of cultures studied MSC karyotype and the level of aneuploidy remained unchanged even after prolonged culturing. The author's experience of ex vivo expansion of MSC using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition of Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of the type of medium on the growth and immunophenotype of MSC is analyzed. The study found that when cultured in serum-free medium proliferative potential of MSCs was lower

SECONDARY SOLID TUMORS IN CHILDREN AFTER ALLOGENEIC HEMATOPOIETIC STEM CELLS TRANSPLANTATION: CASE REPORTS AND LITERATURE REVIEW

Allogeneic hematopoietic stem cell transplantation (HSCT) is an effective approach to cure numerous malignant and nonmalignant diseases. This method is more available now, respectively the number of survivors – recipients of HSCT increases every year. Among long-term complications of HSCT secondary solid cancers are one of the most life-threatening. We discuss different types of secondary solid tumours after HSCT and describe international recommendations of their screening and diagnostic. Several case reports from the single center could illustrate these serious late effects