The "Service" model of the competences of the municipal employee as the basis of vocational training: Russian experience

Competences of the municipal employee are considered in a context of the “service” concept of the public and municipal administration. The results of the sociological researches reflecting estimates and expectations are the basis for a model of the competences, connected with local authorities. In the article the technology and the results of the selection of the applicants for a reserve for the municipal positions are presented on the basis of the offered model of the competences also. From the point of view of the purposes of the vocational training the offered model of the competences can act as a basis for a formation of the curriculum

Hantaviral proteins: Structure, functions, and role in hantavirus infection

© 2015 Muyangwa, Martynova, Khaiboullina, Morzunov and Rizvanov. Hantaviruses are the members of the family Bunyaviridae that are naturally maintained in the populations of small mammals, mostly rodents. Most of these viruses can easily infect humans through contact with aerosols or dust generated by contaminated animal waste products. Depending on the particular Hantavirus involved, human infection could result in either hemorrhagic fever with renal syndrome or in Hantavirus cardiopulmonary syndrome. In the past few years, clinical cases of the Hantavirus caused diseases have been on the rise. Understanding structure of the Hantavirus genome and the functions of the key viral proteins are critical for the therapeutic agents' research. This paper gives a brief overview of the current knowledge on the structure and properties of the Hantavirus nucleoprotein and the glycoproteins

Structural, Semantic and Linguocultural Parameters of Concept Sphere of FASHION INDUSTRY (Modern English Discourse)

This author’s development of the concept sphere model of FASHION INDUSTRY is presented in the article. Lexical units, nominations of objects, subjects and events of the fashion industry, as well as text fragments obtained from modern English-language artistic discourse, media and blogosphere are used as empirical material. The relevance of the study is due to the high rate of transformation of this conceptual field under the influence of a number of extralinguistic factors that make certain changes in the lexical composition of the language, the value dominants of society and the mechanisms of their verbalization. It is shown in the paper that the structure of the concept sphere is anthropocentric, as it is organized around the concept PARTICIPANTS. This concept is not only interconnected with other components of the FASHION INDUSTRY cognitive space — OBJECTS, EVENTS and PROPERTIES — but also determines their semantic content and linguistic and cultural specificity. Particular attention is paid to the actualization of the PROPERTIES component incorporated into other concepts.  Cognitive modeling of the structural and content parameters of the concept sphere made it possible to determine the most highlighted value dominants in the current discourse: innovation, comfort, universality, democracy, mediation with the transition to digital format, economic feasibility and environmental friendliness

Human adipose derived stem cells do not alter cytokine secretion in response to the genetic modification with pEGFP-N2 plasmid DNA

Adipose tissue contain progenitor cells with regenerative (angiogenic, neuroprotection, trophic etc.) potential and treatment based on adipose tissue-derived stem cells (ADSCs) transplantation may constitute a promising therapy. Genetic modification of stem cells with genes encoding growth factors and other biologically active molecules might further increase therapeutic efficiency. However, there is a question about the advantages and disadvantages of such gene/stem cell therapy. For a better understanding of the mechanisms occurring in organism after cell transplantation we should first study cell behavior in vitro. Here we report results of ADSCs transfection with plasmid DNA pEGFP-N2 and subsequent study of cytokines and chemokines secretion using Luminex technology. We demonstrated that genetic modification of ADSCs with commonly used control plasmid vector, encoding enhanced green fluorescent protein (EGFP), did not affect secretion of cytokines/chemokines IFN-{filled circle}, IL-1{filled circle}, IL-2, IL-8, IL-10, IL-12, MCP-1. Thus, genetic modification procedure on its own might have little effect on stem cell properties, making it useful for gene-stem cell therapy applications. © IDOSI Publications, 2013

History of ZIKV infections in India and management of disease outbreaks

© 2007-2018 Frontiers Media S.A. All Rights Reserved. Zika virus (ZIKV) is an emerging arbovirus infection endemic in multiple countries spread from Asia, Africa to the Americas and Europe. Previously known to cause rare and fairly benign human infections, ZIKV has become a major international public health emergency after being linked to unexpected neurological complications, that includes fetal brain damage/death and microcephaly in babies born to infected mothers and Guillain-Barre syndrome (GBS) in adults. It appears that a single genetic mutation in the ZIKV genome, likely acquired during explosive ZIKV outbreak in French Polynesia (2013), made virus causing mild disease to target fetus brain. The Aedes mosquitoes are found to be the main carrier of ZIKV, passing the virus to humans. Originally isolated from patients in Africa in 1954 (African lineage), virus disseminated to Southeast Asia (Asian lineage), establishing new endemic foci, including one in India. Numerous cases of ZIKV infection have been reported in several locations in India and neighboring countries like Pakistan and Bangladesh since mid of the last century, suggesting that the virus reached this part of Asia soon after it was first discovered in Uganda in 1947. Although, the exact means by which ZIKV was introduced to India remains unknown, it appears that the ZIKV strain circulating in India possibly belongs to the "Asian lineage, " which has not yet been associated with microcephaly and other neurological disorders. However, there still exists a threat that the contemporary ZIKV virulent strain from South America, carrying a mutation can return to Asia, posing a potential crisis to newborns and adult patients. Currently there is no specific vaccine or antiviral medication to combat ZIKV infection, thus, vector control and continuous monitoring of potential ZIKV exposure is essential to prevent the devastating consequences similar to the ones experienced in Brazil. However, the major obstacle faced by Indian healthcare agencies is that most cases of ZIKV infection have been reported in rural areas that lack access to rapid diagnosis of infection. In this review, we attempt to present a comprehensive analysis of what is currently known about the ZIKV infection in India and the neighboring countries

Testing Genes Implicated in the Novel Case of Familial Hemiplegic Migraine

© 2016, Springer Science+Business Media New York.Familial hemiplegic migraine (FHM) is a rare monogenetic form of migraine associated with aura and motor dysfunctions. Molecular mechanisms of FHM still remain unknown; however, genetic predisposition to the disease has been suggested. We report several cases of FHM diagnosed in three generations of ethnic Tatars, Russian Federation. Apart from clear family character, patients presented with typical features of FHM including aura and ataxia. In order to determine disease genetic markers in these cases, we sequenced several genes (CACNA1A, ATP1A2, and SCN1A) previously reported in FHM. Sequence analysis demonstrated absence of the previously identified mutations in genes studied. This is the first genetic study of FHM in the Tatar population. The lack of known FHM associated mutation in these patients suggests presence of yet unidentified mutations in some other genes. Further studies including full genome sequencing are needed to clarify the genetic background of FHM cases in the Tatar population

Systemic and local cytokine profile following spinal cord injury in rats: A multiplex analysis

© 2017 Mukhamedshina, Akhmetzyanova, Martynova, Khaiboullina, Galieva and Rizvanov. Our study of the changes in cytokine profile in blood serum and in the spinal cord after traumatic spinal cord injury (SCI) has shown that an inflammatory reaction and immunological response are not limited to the CNS, but widespread. This fact was confirmed by changes detected in a cytokine profile in blood serum samples [MIP-1α, interleukin 1 (IL-1) α, IL-2, IL-5, IL-1β, MCP-1, RANTES]. There were also changes in the levels of MIP-1α, IL-1α, IL-2, IL-5, IL-18, GM-colony-stimulating factor, IL-17α, IFN-γ, IL-10, IL-13, MCP-1, and GRO KC CINC-1 in samples of the rat injured spinal cord. The results underscore the complex cytokine network imbalance exhibited after SCI and show significant changes in the concentrations of 14 cytokines/chemokines with different inflammatory and immunological activities

Inflammatory cytokines kinetics define the severity and phase of nephropathia epidemica

© 2014 Future Medicine Ltd. Aims: Nephropathia epidemica (NE) is a form of hemorrhagic fever with renal syndrome associated with the Puumala virus species of Hantavirus. The pathogenesis of NE is not well understood; therefore, investigating the inflammatory cytokine response to infection may provide useful knowledge in deciphering the pathophysiology of NE. Materials & methods: Using Luminex and ELISA, we analyzed the serum of 137 NE cases and 44 controls to investigate if serum cytokines associate with different clinical presentations. Results: Serum levels of TNF-α and IL-1β are associated with disease severity while upregulation of IL-6, CXCL10, CCL2 and CCL3 are associated with clinical presentation. Conclusion: Inflammatory cytokine kinetics associate with the severity and phase of NE. Our data support a role for inflammatory cytokines in the pathophysiology of NE