Ermittlung von Hintergrundwerten für Grubenwässer des Steinkohlenbergbaus im Saarland

Am Beispiel der Grubenwässer des Saarlandes wurden für die einzelnen Steinkohlengruben Hintergrundwerte mittels der Wahrscheinlichkeitsnetzmethode abgeleitet. Obwohl es sich bei den Grubenwässern um Mischwässer handelt, ist die Ableitung nach dieser Methode technisch möglich und liefert plausible Ergebnisse. Bei einigen Bergwerken wurden insbesondere für die Parameter elektrische Leitfähigkeit und Chlorid zwei Populationen festgestellt. Die Grubenwässer westlich der Saar sind gegenüber denen nordöstlich des Vorfluters durch höhere Mineralisationen gekennzeichnet.Abstract: Using the example of the mine waters of the Saarland, background values were derived for the individual coal mines using the probability network method. Despite the fact that the mine waters are mixed waters, deriving values using this method is technically feasible and yields plausible results. In some mines, two populations were identified, particularly for the parameters of electrical conductivity and chloride. The mine waters west of the Saar River are characterized by higher mineralizations compared to those located northeast of the watercourse.researc

Nuclear localization signal and protein context both mediate importin α specificity of nuclear import substrates

The 'classical' nuclear protein import pathway depends on importin alpha and importin beta. Importin alpha binds nuclear localization signal (NLS)-bearing proteins and functions as an adapter to access the importin beta dependent import pathway. In humans only one importin beta is known to interact with importin alpha, while six alpha importins have been described. Various experimental approaches provided evidence that several substrates are transported specifically by particular alpha importins. Whether or not the NLS is sufficient to mediate importin alpha specificity is unclear. To address this question, we exchanged the NLS of two well-characterized import substrates, the seven-bladed propeller protein RCC1, preferentially transported into the nucleus by importin alpha3, and the less specifically imported substrate nucleoplasmin. In vitro binding studies and nuclear import assays revealed that both NLS and protein context contribute to specificity of importin alpha binding and transport