321 research outputs found
The Cyclin-Dependent Kinase 5 Inhibitor Peptide Inhibits Herpes Simplex Virus Type 1 Replication
© 2019, The Author(s). In order to evaluate the influence of CDK5 inhibitory peptide (CIP) on Human alphaherpesvirus 1 (HSV-1) replication, we constructed two recombinant adeno-associated-virus 2 (rAAV2) vectors encoding CIP fused with cyan-fluorescent-protein (CFP), with or without nuclear localization signal. A third vector encoding non-fused CIP and CFP was also constructed. HeLa and HEK 293T cells were infected with the rAAV-CIP vectors at multiplicity of infection (MOI) of 5000, in the absence or presence of a recombinant HSV-1 that encodes a yellow-fluorescent-protein (rHSV48Y; MOI = 1). Cells co-infected with rHSV48Y and rAAV vectors that did not express the CIP gene (rAAV-CFP-Neo) served as controls. At 24 h after infection, the effect of CIP on rHSV48Y replication was assessed by PCR, qRT-PCR, Western-blot, flow-cytometry, epifluorescence and confocal microscopy. We show that in cultures co-infected with rAAV-CFP-Neo, 27% of the CFP-positive cells present rHSV48Y replication compartments. By contrast, in cultures co-infected with CIP-encoding rAAV2 vectors and rHSV48Y only 6–20% of the cells positive for CIP showed rHSV48Y replication compartments, depending on the CIP variant. Flow-cytometry showed that less than 40% of the rHSV48Y/rAAV-CIP, and more than 75% of rHSV48Y/rAAV-CFP-Neo co-infected cells were positive for both transgene products. The microscopy and flow-cytometry data support the hypothesis that CIP is inhibiting HSV-1 replication
Stem cell therapies in preclinical models of stroke. Is the aged brain microenvironment refractory to cell therapy?
© 2017.Stroke is a devastating disease demanding vigorous search for new therapies. Initial enthusiasm to stimulate restorative processes in the ischemic brain by means of cell-based therapies has meanwhile converted into a more balanced view recognizing impediments that may be related to unfavorable age-associated environments. Recent results using a variety of drug, cell therapy or combination thereof suggest that, (i) treatment with Granulocyte-Colony Stimulating Factor (G-CSF) in aged rats has primarily a beneficial effect on functional outcome most likely via supportive cellular processes such as neurogenesis; (ii) the combination therapy, G-CSF with mesenchymal cells (G-CSF + BM-MSC or G-CSF + BM-MNC) did not further improve behavioral indices, neurogenesis or infarct volume as compared to G-CSF alone in aged animals; (iii) better results with regard to integration of transplanted cells in the aged rat environment have been obtained using iPS of human origin; (iv) mesenchymal cells may be used as drug carriers for the aged post-stroke brains. Conclusion: While the middle aged brain does not seem to impair drug and cell therapies, in a real clinical practice involving older post-stroke patients, successful regenerative therapies would have to be carried out for a much longer time
Global Existence and Regularity for the 3D Stochastic Primitive Equations of the Ocean and Atmosphere with Multiplicative White Noise
The Primitive Equations are a basic model in the study of large scale Oceanic
and Atmospheric dynamics. These systems form the analytical core of the most
advanced General Circulation Models. For this reason and due to their
challenging nonlinear and anisotropic structure the Primitive Equations have
recently received considerable attention from the mathematical community.
In view of the complex multi-scale nature of the earth's climate system, many
uncertainties appear that should be accounted for in the basic dynamical models
of atmospheric and oceanic processes. In the climate community stochastic
methods have come into extensive use in this connection. For this reason there
has appeared a need to further develop the foundations of nonlinear stochastic
partial differential equations in connection with the Primitive Equations and
more generally.
In this work we study a stochastic version of the Primitive Equations. We
establish the global existence of strong, pathwise solutions for these
equations in dimension 3 for the case of a nonlinear multiplicative noise. The
proof makes use of anisotropic estimates, estimates on the
pressure and stopping time arguments.Comment: To appear in Nonlinearit
First results of the air shower experiment KASCADE
The main goals of the KASCADE (KArlsruhe Shower Core and Array DEtector)
experiment are the determination of the energy spectrum and elemental
composition of the charged cosmic rays in the energy range around the knee at
ca. 5 PeV. Due to the large number of measured observables per single shower a
variety of different approaches are applied to the data, preferably on an
event-by-event basis. First results are presented and the influence of the
high-energy interaction models underlying the analyses is discussed.Comment: 3 pages, 3 figures included, to appear in the TAUP 99 Proceedings,
Nucl. Phys. B (Proc. Suppl.), ed. by M. Froissart, J. Dumarchez and D.
Vignau
Electron, Muon, and Hadron Lateral Distributions Measured in Air-Showers by the KASCADE Experiment
Measurements of electron, muon, and hadron lateral distributions of extensive
air showers as recorded by the KASCADE experiment are presented. The data cover
the energy range from about 5x10^14 eV up to almost 10^17 eV and extend from
the inner core region to distances of 200 m. The electron and muon
distributions are corrected for mutual contaminations by taking into account
the detector properties in the experiment. All distributions are well described
by NKG-functions. The scale radii describing the electron and hadron data best
are approx. 30 m and 10 m, respectively. We discuss the correlation between
scale radii and `age' parameter as well as their dependence on shower size,
zenith angle, and particle energy threshold.Comment: 28 pages, 14 figures, Accepted for publication in Astroparticle
Physic
CONSTRUCTION EVOLUTION OF DEEP SOIL LOOSENING EQUIPMENT IN ROMANIA IN THE COUNTRY SPECIFIC SOIL CONDITIONS
This paper presents the main achievements of the largest manufacturers of deep soil loosening machines and equipment, highlighting the specific characteristics of each device, so that they achieve a very good job loosening in depth, using an low energy source for the same working width
KCDC - The KASCADE Cosmic-ray Data Centre
KCDC, the KASCADE Cosmic-ray Data Centre, is a web portal, where data of
astroparticle physics experiments will be made available for the interested
public. The KASCADE experiment, financed by public money, was a large-area
detector for the measurement of high-energy cosmic rays via the detection of
air showers. KASCADE and its extension KASCADE-Grande stopped finally the
active data acquisition of all its components including the radio EAS
experiment LOPES end of 2012 after more than 20 years of data taking. In a
first release, with KCDC we provide to the public the measured and
reconstructed parameters of more than 160 million air showers. In addition,
KCDC provides the conceptional design, how the data can be treated and
processed so that they are also usable outside the community of experts in the
research field. Detailed educational examples make a use also possible for
high-school students and early stage researchers.Comment: 8 pages, accepted proceeding of the ECRS-symposium, Kiel, 201
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