Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Thalassemia mutations in Gaziantep, Turkey

Ninety-eight postnatal and six prenatal cases of thalassemia were studied by the reverse dot-blot hybridization technique in the city of Gaziantep, Turkey. We found the following mutations: IVS 1.110 (G>A) in 29.1%, IVS 2.1 (G>A) in 12.3%, IVS 1.1 (G>A) in 7.7%, Codon 8 (-AA) in 5.6%, -30 (T>A) in 4.6%, IVS 1.6 (T>C) in 4.6%, Codon 39 (C>T) in 3.6%, Codon 44 (-C) in 3.1%, IVS 2.745 (C>G) in 1.5%, Codon 8/9 (+G) in 2.1%, Codon 36/37 (-T) in 2.1%, IVS 1.5 (G>C) in 2.1%, Codon 22 (7pb del) in 0.5%, Codon 5 (-CT) in 0.5% while 20.9% were undetermined. 54 of the thalassemia patients were homozygotes, 12 were compound heterozygous and 31 were heterozygotes. In one allele of 5 thalassemia patients, - thalassemia mutation (3.7 single gene deletions in 1 patient, anti-3.7 gene triplication in 4 patients) wasdetermined at the same time. Finally, this is the first comprehensive study in this region and percentage of and - globin genes mutation is 2.6 and 79.4%, respectively

Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Interpolation of SUSY quantum mechanics

Interpolation of two adjacent Hamiltonians in SUSY quantum mechanics $H_s=(1-s)A^{\dagger}A + sAA^{\dagger}$, $0\le s\le 1$ is discussed together with related operators. For a wide variety of shape-invariant degree one quantum mechanics and their `discrete' counterparts, the interpolation Hamiltonian is also shape-invariant, that is it takes the same form as the original Hamiltonian with shifted coupling constant(s).Comment: 18 page

Assessment of fetal adrenal gland enlargement in term and preterm labor cases

Background: The objective of this study was to compare the Fetal Zone Depth (FZD) of fetal adrenal gland in term and preterm labor cases.  Methods: Twenty nine preterm pregnant women at 29-36 weeks of gestation with single pregnancy admitted with the clinical diagnosis of preterm labor and the comparison group of 33 pregnant women at 37-40 weeks with term pregnancy were included in this study. FZD and Total Gland Depth (TGD) of fetal adrenal gland of the entire fetuses in sagittal plane were ultrasonographically measured and FZD/TGD ratios were calculated. Demographic and clinical features, laboratory findings and fetal adrenal gland FZD/TGD ratios were compared between the two groups.Results: No difference was found between the two groups in respect of age, number of pregnancies and delivery method (P >0.05); yet preterm birth history and duration of hospital stay were higher in preterm group (P <0.05). Fetal adrenal gland FZD/TGD ratio was statistically significantly higher in preterm group compared to the term group (55.4% ± 4.9 vs. 47.7% ± 5.6; P <0.001).Conclusion: The growth in FZ as a fetal adaptation mechanism in increased fetal stress in preterm labor cases was at a significant level. Once supported by more comprehensive studies, we think that this result would be beneficial in the prediction of preterm labor in clinical practice.

Method development and validation for the determination of cabergoline in tablets by capillary zone electrophoresis

A capillary zone electrophoresis (CZE) method has been developed for the analysis of cabergoline in its pharmaceutical preparations. Optimized analysis conditions for cabergoline analysis were performed using 110 mM pH 5.0 phosphate buffer containing 30 % acetonitrile as an electrolyte solution. Separation was performed through a fused silica capillary (50 μm i.d., total length 64.5 cm, 50.0 cm effective length) at 30 ºC with an applied voltage of 30 kV and hydrodynamic injection for 4 s. Cabergoline and internal standard verapamil were detected at a wavelength of 220 nm. The calibration was liner from 5.0 to 90.0 μg mL–1 and the limit of detection and quantification were 1.25 and 3.77 μg mL–1 Optimized CE . method was validated on the basis of related ICH guideline and found as an accurate, sensitive, precise and reproducible method for cabergoline determination. Developed method is also successfully applied for the analysis of pharmaceutical preparations containing cabergoline.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Polymorphisms of the DNA repair gene XPD (751) and XRCC1 (399) correlates with risk of hematological malignancies in Turkish population

Polymorphisms that occur in DNA repair genes affect DNA repair capacity and constitute a risk factor in hematological malignancies. This study, was aimed to investigate whether xeroderma pigmentosum complementation group D (XPD) and x-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms were involved in the susceptibility to different hematological malignancies. The genotype and allele frequencies were obtained by analyzing XPD gene codon 751 in a total of 80 patients and XRCC1 gene codon 399 polymorphism in a total of 100 patients with hematological malignancies and 100 healthy controls. Mean age was 45 (range: 16 to 75) and 46 (range: 16 to 82) in the patients groups and 39.5 (range: 18 to 67) in the control group, respectively. Additionally, distribution of genotypes and alleles were compared in the patient and control groups. In the comparison of genotype and allele frequencies in hematological malignancies and healthy controls, XPD-751Gln variant was arranged and compared according to age and sex and Gln/Gln genotype was reported to be a protector, which was decreased significantly in acute myeloblastic leukemia (AML) (p = 0.042). No relationship was determined between allele frequencies (p = 0.054). In XRCC1-399, it was shown that Gln/Gln genotype was decreased significantly in AML (p = 0.014) plus all hematological malignancies (p = 0.033) and that Gln allele was present at a lower ratio in AML (p = 0.046). The distribution of polymorphism of both genes was not statistically significant in terms of age and sex. In leukemia with early relapse, XPD 751 Lys/Lys genotype was determined at a statistically higher ratio (p = 0.042). In the evaluation of both genes together, a decrease was noted in Gln/Gln + Lys/Gln haplotype frequency in hematological malignancies (p = 0.048). In this study, it was demonstrated that a decrease in Gln/Gln genotype and Gln allele acted as a protector in XPD codon 751 and XRCC1 codon 399 polymorphisms in acute myeloblastic leukemia (AML) and that an increase in Lys/Lys genotype in acute leukemia was associated with early relapse

CHANGES OF THYROID HORMONES IN DIFFERENT PHYSIOLOGICAL PERIODS IN WHITE GOATS

ABSTRACT The levels of thyroid hormones are important indicator of metabolic activity. The knowledge of the metabolic activity in different physiological periods, animals readjusted dietary regimen. Therefore, in this study, changes of triiodotironine (T3) and thyroxine (T4) hormones in the blood serum of female (n=14) and male (n=9) white goats were studied for a duration of one year through different physiological periods [breeding (September-October), gestation (November to March), postpartum-sucking (April-May) and milking (June to August)]. Results show that the differences in T3 and T4 hormone levels between sexes are statistically unimportant in breeding, gestation, postpartum-sucking and milking periods, while the changes for each sex between the physiological periods are important (p&lt;0.05). Also, it has been found out that the thyroid hormone levels in different physiological periods are under the influence of the environmental temperature changes

Do Peripheral and Extra Musculoskeletal Manifestations Have An Impact on Biologic Dmard Prescribing Patterns in Axial Spondyloarthritis: the Results of Treasure Experience

[No Abstract Available

Tool flank wear prediction using high-frequency machine data from industrial edge device

Tool flank wear monitoring can minimize machining downtime costs while increasing productivity and product quality. In some industrial applications, only a limited level of tool wear is allowed to attain necessary tolerances. It may become challenging to monitor a limited level of tool wear in the data collected from the machine due to the other components, such as the flexible vibrations of the machine, dominating the measurement signals. In this study, a tool wear monitoring technique to predict limited levels of tool wear from the spindle motor current and dynamometer measurements is presented. High-frequency spindle motor current data is collected with an industrial edge device while the cutting forces and torque are measured with a rotary dynamometer in drilling tests for a selected number of holes. Feature engineering is conducted to identify the statistical features of the measurement signals that are most sensitive to small changes in tool wear. A neural network based on the long short-term memory (LSTM) architecture is developed to predict tool flank wear from the measured spindle motor current and dynamometer signals. It is demonstrated that the proposed technique predicts tool flank wear with good accuracy and high computational efficiency. The proposed technique can easily be implemented in an industrial edge device as a real-time predictive maintenance application to minimize the costs due to manufacturing downtime and tool underuse or overuse.Comment: The first four authors have equal contributio