Nicotinic receptor activation induces NMDA‐receptor independent long‐term potentiation of glutamatergic signalling in hippocampal oriens interneurons

Many interneurons located in stratum oriens of the rodent hippocampus exhibit a form of long‐term potentiation (LTP) of glutamatergic transmission that does not depend on NMDA receptors for its induction but, instead, requires Ca2+‐permeable AMPA receptors and group I metabotropic glutamate receptors. A role for cholinergic signalling has also been reported. However, electrical stimulation of presynaptic axons, conventionally used to evoke synaptic responses, does not allow the relative roles of glutamatergic and cholinergic synapses in the induction of LTP to be distinguished. Here, we show that repetitive optogenetic stimulation confined to cholinergic axons is sufficient to trigger a lasting potentiation of glutamatergic signalling. This phenomenon shows partial occlusion with LTP induced by electrical stimulation, and is sensitive to postsynaptic Ca2+ chelation and blockers of nicotinic receptors. ACh release from cholinergic axons is thus sufficient to trigger heterosynaptic potentiation of glutamatergic signalling to oriens interneurons in the hippocampus

T-type calcium channels contribute to NMDA receptor-independent synaptic plasticity in hippocampal regular-spiking oriens-alveus interneurons.

NMDA receptor-independent long-term potentiation (LTP) in hippocampal stratum oriens-alveus (O/A) interneurons requires co-activation of postsynaptic group I metabotropic glutamate receptors (mGluRs) and Ca(2+) -permeable AMPA receptors. The rectification properties of such AMPA receptors contribute to the preferential induction of LTP at hyperpolarized potentials. A persistent increase in excitatory transmission can also be triggered by exogenous activation of group I mGluRs while the interneuron is hyperpolarized, or by postsynaptic trains of action potentials in the absence of presynaptic stimulation. Here we identify low-threshold transient (T-type) channels as a further source of Ca(2+) that contributes to synaptic plasticity. T-type Ca(2+) currents were detected in mouse regular-spiking O/A interneurons. Blocking T-type currents pharmacologically prevented LTP induced by high-frequency stimulation of glutamatergic axons, or by application of the group I mGluR agonist dihydroxyphenylglycine (DHPG), paired with postsynaptic hyperpolarization. T-type current blockade also prevented synaptic potentiation induced by postsynaptic action potential trains. Several sources of Ca(2+) thus converge on NMDA receptor-independent LTP induction in O/A interneurons. This article is protected by copyright. All rights reserved

ON THE DEVELOPMENT OF A DATASET PUBLICATION GUIDELINE: DATA REPOSITORIES AND KEYWORD ANALYSIS IN ISPRS DOMAIN

The FAIR principle (find, access, interoperability, reuse) forms a sustainable resource for scientific exchange between researchers. Currently, the implementation of this principle is an important process for future research projects. To support this process in the ISPRS community, the usage of data repositories for dataset publication has the potential to bring closer the achievement of the FAIR principle. Therefore, we (1) analysed available data repositories, (2) identified common keywords in ISPRS publications and (3) developed a tool for searching appropriate repositories. Thus, infrastructures from the field of geosciences, that can already be used, become more accessible

Induction of Anti-Hebbian LTP in CA1 Stratum Oriens Interneurons: Interactions between Group I Metabotropic Glutamate Receptors and M1 Muscarinic Receptors

An anti-Hebbian form of LTP is observed at excitatory synapses made with some hippocampal interneurons. LTP induction is facilitated when postsynaptic interneurons are hyperpolarized, presumably because Ca(2+) entry through Ca(2+)-permeable glutamate receptors is enhanced. The contribution of modulatory transmitters to anti-Hebbian LTP induction remains to be established. Activation of group I metabotropic receptors (mGluRs) is required for anti-Hebbian LTP induction in interneurons with cell bodies in the CA1 stratum oriens. This region receives a strong cholinergic innervation from the septum, and muscarinic acetylcholine receptors (mAChRs) share some signaling pathways and cooperate with mGluRs in the control of neuronal excitability.We therefore examined possible interactions between group I mGluRs and mAChRs in anti-Hebbian LTP at synapses which excite oriens interneurons in rat brain slices. We found that blockade of either group I mGluRs or M1 mAChRs prevented the induction of anti-Hebbian LTP by pairing presynaptic activity with postsynaptic hyperpolarization. Blocking either receptor also suppressed long-term effects of activation of the other G-protein coupled receptor on interneuron membrane potential. However, no crossed blockade was detected for mGluR or mAchR effects on interneuron after-burst potentials or on the frequency of miniature EPSPs. Paired recordings between pyramidal neurons and oriens interneurons were obtained to determine whether LTP could be induced without concurrent stimulation of cholinergic axons. Exogenous activation of mAChRs led to LTP, with changes in EPSP amplitude distributions consistent with a presynaptic locus of expression. LTP, however, required noninvasive presynaptic and postsynaptic recordings

CURRENT STATUS OF THE BENCHMARK DATABASE BEMEDA

Open science is an important attribute for developing new approaches. Especially, the data component plays a significant role. The FAIR principle provides a good orientation towards open data. One part of FAIR is findability. Thus, domain specific dataset search platforms were developed: the Earth Observation Database and our Benchmark Metadata Database (BeMeDa). In addition to the search itself, the datasets found by this platforms can be compared with each other with regard to their interoperability. We compare these two platforms and present an update of our platform BeMeDa. This update includes additional location information about the datasets and a new frontend design with improved usability. We rely on user feedback for further improvements and enhancements

Long-term potentiation in hippocampal oriens interneurons: postsynaptic induction, presynaptic expression and evaluation of candidate retrograde factors

Several types of hippocampal interneurons exhibit a form of long-term potentiation (LTP) that depends on Ca(2+)-permeable AMPA receptors and group I metabotropic glutamate receptors. Several sources of evidence point to a presynaptic locus of LTP maintenance. The retrograde factor that triggers the expression of LTP remains unidentified. Here, we show that trains of action potentials in putative oriens-lacunosum-moleculare interneurons of the mouse CA1 region can induce long-lasting potentiation of stimulus-evoked excitatory postsynaptic currents that mimics LTP elicited by high-frequency afferent stimulation. We further report that blockers of nitric oxide production or TRPV1 receptors failed to prevent LTP induction. The present results add to the evidence that retrograde signalling underlies N-methyl-d-aspartate (NMDA) receptor-independent LTP in oriens interneurons, mediated by an unidentified factor

Preparation of dissociated mouse primary neuronal cultures from long-term cryopreserved brain tissue

BACKGROUND: Dissociated primary neuronal cultures are widely used as a model system to investigate the cellular and molecular properties of diverse neuronal populations and mechanisms of action potential generation and synaptic transmission. Typically, rodent primary neuronal cultures are obtained from freshly-dissociated embryonic or postnatal brain tissue, which often requires intense animal husbandry. This can strain resources when working with genetically modified mice. NEW METHOD: Here we describe an experimental protocol for frozen storage of mouse hippocampi, which allows fully functional dissociated primary neuronal cultures to be prepared from cryopreserved tissue. RESULTS: We show that thawed hippocampal neurons have functional properties similar to those of freshly dissociated neurons, including neuronal morphology, excitability, action potential waveform and synaptic neurotransmitter release, even after cryopreservation for several years. COMPARISON TO THE EXISTING METHODS: In contrast to the existing methods, the protocol described here allows for efficient long-term storage of samples, allowing researchers to perform functional experiments on neuronal cultures from brain tissue collected in other laboratories. CONCLUSIONS: We anticipate that this method will facilitate collaborations among laboratories based at distant locations and will thus optimise the use of genetically modified mouse models, in line with the 3Rs (Replacement, Reduction and Refinement) recommended for scientific use of animals in research

Dynamical ejecta of neutron star mergers with nucleonic weak processes II: Kilonova emission

The majority of existing results for the kilonova (or macronova) emission from material ejected during a neutron-star (NS) merger is based on (quasi-)one-zone models or manually constructed toy-model ejecta configurations. In this study we present a kilonova analysis of the material ejected during the first ~10ms of a NS merger, called dynamical ejecta, using directly the outflow trajectories from general relativistic smoothed-particle hydrodynamics simulations including a sophisticated neutrino treatment and the corresponding nucleosynthesis results, which have been presented in Part I of this study. We employ a multi-dimensional two-moment radiation transport scheme with approximate M1 closure to evolve the photon field and use a heuristic prescription for the opacities found by calibration with atomic-physics based reference results. We find that the photosphere is generically ellipsoidal but augmented with small-scale structure and produces emission that is about 1.5-3 times stronger towards the pole than the equator. The kilonova typically peaks after 0.7-1.5days in the near-infrared frequency regime with luminosities between 3-7x10^40erg/s and at photospheric temperatures of 2.2-2.8x10^3K. A softer equation of state or higher binary-mass asymmetry leads to a longer and brighter signal. Significant variations of the light curve are also obtained for models with artificially modified electron fractions, emphasizing the importance of a reliable neutrino-transport modeling. None of the models investigated here, which only consider dynamical ejecta, produces a transient as bright as AT2017gfo. The near-infrared peak of our models is incompatible with the early blue component of AT2017gfo.Comment: 23 pages, 15 figures, 1 table, accepted to MNRAS; updated for correct version of Fig. A1, left panel, and corrected Eqs. 7 and

Data clustering and noise undressing for correlation matrices

We discuss a new approach to data clustering. We find that maximum likelihood leads naturally to an Hamiltonian of Potts variables which depends on the correlation matrix and whose low temperature behavior describes the correlation structure of the data. For random, uncorrelated data sets no correlation structure emerges. On the other hand for data sets with a built-in cluster structure, the method is able to detect and recover efficiently that structure. Finally we apply the method to financial time series, where the low temperature behavior reveals a non trivial clustering.Comment: 8 pages, 5 figures, completely rewritten and enlarged version of cond-mat/0003241. Submitted to Phys. Rev.