Utjecaj promijenjene mineralne ishrane na iglice Pančićeve omorike (Picea omorika Pančić) – istraživanje pojedinačnog slučaja

A widespread conifer forest decline in Europe and North America has been expressed through various combinations of symptoms. In our study, unspecific biochemical indicators and histological findings were compared with visually assessed damage symptoms in order to get a detailed diagnosis of a single declining Picea omorika Pančić tree. By measuring the content of photosynthetic pigments and proteins as well as total guaiacol peroxidase activity we confirmed an altered physiological state of the investigated tree. Histological analysis showed specific patterns of decline for Mg and K deficient trees.Sveprisutno propadanje šuma u Europi i sjevernoj Americi manifestira se kao kombinacija različitih simptoma. U provedenom istraživanju usporedili smo nespecifične biokemijske indikatore i histološke nalaze s vizualnom procjenom oštećenja, a s ciljem postavljanja detaljne dijagnoze propadanja jednog stabla Pančićeve omorike (Picea omorika Pančić). Odre|ivanjem sadržaja fotosintetskih pigmenata, proteina i aktivnosti ukupnih gvajacilnih peroksidaza potvr|ena je promjena fiziološkog statusa biljke. Histološke analize pokazale su specifičnosti karakteristične za stabla deficijentna magnezijem i kalijem

Utjecaj promijenjene mineralne ishrane na iglice Pančićeve omorike (Picea omorika Pančić) – istraživanje pojedinačnog slučaja

A widespread conifer forest decline in Europe and North America has been expressed through various combinations of symptoms. In our study, unspecific biochemical indicators and histological findings were compared with visually assessed damage symptoms in order to get a detailed diagnosis of a single declining Picea omorika Pančić tree. By measuring the content of photosynthetic pigments and proteins as well as total guaiacol peroxidase activity we confirmed an altered physiological state of the investigated tree. Histological analysis showed specific patterns of decline for Mg and K deficient trees.Sveprisutno propadanje šuma u Europi i sjevernoj Americi manifestira se kao kombinacija različitih simptoma. U provedenom istraživanju usporedili smo nespecifične biokemijske indikatore i histološke nalaze s vizualnom procjenom oštećenja, a s ciljem postavljanja detaljne dijagnoze propadanja jednog stabla Pančićeve omorike (Picea omorika Pančić). Odre|ivanjem sadržaja fotosintetskih pigmenata, proteina i aktivnosti ukupnih gvajacilnih peroksidaza potvr|ena je promjena fiziološkog statusa biljke. Histološke analize pokazale su specifičnosti karakteristične za stabla deficijentna magnezijem i kalijem

Total soluble proteins amount in vegetative buds and needles of norway spruce during the bursting time

The vegetative buds and later on young needles as well as needles formed in spring previous year of Picea abieswere used in the experimental work. Extracted proteins were determined spectrophotometrically and the amount of dry weight was measured. The data revealed that the dry weight of needles formed in spring previous year was about three times higher than in the vegetative buds at the beginning of investigations. During the sampling period the dry weight in those needles was keeping nearly the same level (39-54%). The increase in dry weight was noticed in the young needles 5-6 weeks after vegetative buds burst. The amount of total soluble proteins in the needles formed previous year was about 140 mg/g of dry weight and it was uniformed during the investigation period. In the vegetative buds the amount of proteins was three times higher than in needles. But, the concentration starts to decrease just before bursting of the vegetative buds as well as during next 2 or 3 weeks when young shoot proliferated. After this decreasing period amount of proteins in newly formed needles briefly reached (in 2 weeks period) the level as it was in vegetative buds. In the following period the decrease of water amount as well as the protein amount in young needles was observed

Metal Bioavailability in the Sava River Water

Metals present one of the major contamination problems for freshwater systems, such as the Sava River, due to their high toxicity, persistence, and tendency to accumulate in sediment and living organisms. The comprehensive assessment of the metal bioavailability in the Sava River encompassed the analyses of dissolved and DGT-labile metal species of nine metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in the river water, as well as the evaluation of the accumulation of five metals (Cd, Cu, Fe, Mn, and Zn) in three organs (liver, gills, and gastrointestinal tissue) of the bioindicator organism, fish species European chub (Squalius cephalus L.).This survey was conducted mainly during the year 2006, in two sampling campaigns, in April/May and September, as periods representative for chub spawning and post-spawning. Additionally, metal concentrations were determined in the intestinal parasites acanthocephalans, which are known for their high affinity for metal accumulation. Metallothionein concentrations were also determined in three chub organs, as a commonly applied biomarker of metal exposure. Based on the metal concentrations in the river water, the Sava River was defined as weakly contaminated and mainly comparable with unpolluted rivers, which enabled the analyses of physiological variability of metal and metallothionein concentrations in the chub organs, as well as the establishment of their constitutive levels