Estandarización de dos PCR multiplex como base de un test de genotipado para pejerrey : Odontesthes bonariensis

El pejerrey bonaerense es una especie de gran importancia económica en Argentina. La piscicultura de pejerrey data de varias décadas en las que se ha poblado o repoblado ambientes con larvas. A pesar de la importancia de esta especie, la información sobre la diversidad genética de sus poblaciones, que sirva para la mejora de su producción o para la mitigación del impacto de las resiembras, es escasa. En este trabajo se propone el desarrollo de un test de genotipado basado en dos reacciones de PCR multiplex utilizando marcadores microsatélites. Se seleccionaron 13 marcadores descriptos en la bibliografía que fueron estandarizados. Luego, los marcadores se evaluaron en una población silvestre (n=20). Se extrajo el ADN a partir de tejido de cada individuo y se amplifico por PCR los 13 marcadores microsatélites. Los productos de amplificación fueron resueltos en geles de poliacrilamida teñidos con plata. Para cada individuo se obtuvo un genotipo. Para cada locus, se determinó el número de alelos, heterocigocidad observada y esperada, frecuencia de alelos nulos, contenidos de información polimórfica (PIC), y probabilidad de exclusión. Teniendo en cuenta estos parámetros, se seleccionaron 7 loci que fueron combinados en dos PCR multiplex M1 y M2 de acuerdo a los tamaños de los fragmentos que produce su amplificación. Los cebadores se marcaron fluorescentemente, y se estandarizaron las condiciones para M1 y M2. Las amplificaciones se evaluaron por electroforesis capilar. Dichas multiplex constituyen la base del Test de genotipado y grado de parentesco para pejerrey. Esta herramienta permitirá evaluar la diversidad genética y el grado de parentesco existente entre los reproductores de los centros de cultivo evitando la depresión consanguíneaFil: Sapino, Román. Facultad de Ciencias y Bioquímicas - Universidad Nacional de RosarioFil: Arranz, Silvia E. Facultad de Ciencias y Bioquímicas - Universidad Nacional de RosarioFil: Diaz, Juan. Facultad de Ciencias y Bioquímicas - Universidad Nacional de RosarioFil: Faggiani, Mariano. Centro Científico, Tecnológico y Educativo "Acuario del Río Paraná"Fil: Villanova, Gabriela V. Centro Científico, Tecnológico y Educativo "Acuario del Río Paraná

Modelling low velocity impact induced damage in composite laminates

The paper presents recent progress on modelling low velocity impact induced damage in fibre reinforced composite laminates. It is important to understand the mechanisms of barely visible impact damage (BVID) and how it affects structural performance. To reduce labour intensive testing, the development of finite element (FE) techniques for simulating impact damage becomes essential and recent effort by the composites research community is reviewed in this work. The FE predicted damage initiation and propagation can be validated by Non Destructive Techniques (NDT) that gives confidence to the developed numerical damage models. A reliable damage simulation can assist the design process to optimise laminate configurations, reduce weight and improve performance of components and structures used in aircraft construction

Modelling low velocity impact induced damage in composite laminates

The paper presents recent progress on modelling low velocity impact induced damage in fibre reinforced composite laminates. It is important to understand the mechanisms of barely visible impact damage (BVID) and how it affects structural performance. To reduce labour intensive testing, the development of finite element (FE) techniques for simulating impact damage becomes essential and recent effort by the composites research community is reviewed in this work. The FE predicted damage initiation and propagation can be validated by Non Destructive Techniques (NDT) that gives confidence to the developed numerical damage models. A reliable damage simulation can assist the design process to optimise laminate configurations, reduce weight and improve performance of components and structures used in aircraft construction

Merging blue green Infrastructure and biotechnology: the case of glyphosate in Santa Lucia’s sub-basin

In combination with Blue Green Infrastructure, microorganisms can be used as bioremediation agents for glyphosate, facilitated by immobilization on inert matrices for subsequent removal. In the present work, the usefulness hydrological tools in targeting and designing a glyphosate bioremediation strategy was evaluated together with the isolation of glyphosate-degrading bacteria

On the feasibility of measuring the internet through smartphone-based crowdsourcing

The large base of Internet-enabled smartphones provides an excellent opportunity for a fine-grained observation of the structure of the Internet and a quantitative evaluation of its characteristics. Smartphones can operate as active mobile monitors and, coordinated by a central entity, they can probe the network on a local scale. Then the results produced by a large number of participants can be merged to obtain a detailed graph of the Internet. Besides the design of the measurement framework, this paper describes the implementation and validation of a traceroute-like tool that is compatible with the Android platform. This confirms that smartphone-based crowdsourcing of network properties can be a viable strategy