The Making of the English People

"The particular subject of this article is the making of the English people…

Genetic and Functional Analyses of PptA, a Phospho-Form Transferase Targeting Type IV Pili in Neisseria gonorrhoeae

The PilE pilin subunit protein of Neisseria gonorrhoeae undergoes unique covalent modifications with phosphoethanolamine (PE) and phosphocholine (PC). The pilin phospho-form transferase A (PptA) protein, required for these modifications, shows sequence relatedness with and architectural similarities to lipopolysaccharide PE transferases. Here, we used regulated expression and mutagenesis as means to better define the relationships between PptA structure and function, as well as to probe the mechanisms by which other factors impact the system. We show here that pptA expression is coupled at the level of transcription to its distal gene, murF, in a division/cell wall gene operon and that PptA can act in a dose-dependent fashion in PilE phospho-form modification. Molecular modeling and site-directed mutagenesis provided the first direct evidence that PptA is a member of the alkaline phosphatase superfamily of metalloenzymes with similar metal-binding sites and conserved structural folds. Through phylogenetic analyses and sequence alignments, these conclusions were extended to include the lipopolysaccharide PE transferases, including members of the disparate Lpt6 subfamily, and the MdoB family of phosphoglycerol transferases. Each of these enzymes thus likely acts as a phospholipid head group transferase whose catalytic mechanism involves a trans-esterification step generating a protein-phospho-form ester intermediate. Coexpression of PptA with PilE in Pseudomonas aeruginosa resulted in high levels of PE modification but was not sufficient for PC modification. This and other findings show that PptA-associated PC modification is governed by as-yet-undefined ancillary factors unique to N. gonorrhoeae

High export of dissolved silica from the Greenland Ice Sheet

Silica is an essential element for marine life and plays a key role in the biogeochemistry of the ocean. Glacial activity stimulates rock weathering, generating dissolved silica that is exported to coastal areas along with meltwater. The magnitude of the dissolved silica export from large glacial areas such as the Greenland Ice Sheet is presently poorly quantified and not accounted for in global budgets. Here we present data from two fjord systems adjacent to the Greenland Ice Sheet which reveal a large export of dissolved silica by glacial meltwater relative to other macronutrients. Upscaled to the entire Greenland Ice Sheet, the export of dissolved silica equals 22 ± 10 Gmol Si yr−1. When the silicate-rich meltwater mixes with upwelled deep water, either inside or outside Greenland's fjords, primary production takes place at increased silicate to nitrate ratios. This likely stimulates the growth of diatoms relative to other phytoplankton groups

ØKOKYST – DP Norskehavet Sør, Årsrapport 2022

Prosjektleder: Elianne EggeOvervåkingsprogrammet "Økosystemovervåking i Kystvann – Økokyst" har til hensikt å overvåke miljøtilstanden langs norskekysten i henhold til vannforskriften. Økokyst delprogram Norskehavet Sør dekker kyststrekningen fra Ulsteinvik i sør til Helgeland i nord. Av de ni vannforekomstene som kunne klassifiseres i 2022 fikk én samlet «svært god» tilstand, sju «god» tilstand, mens én fikk «moderat» tilstand, der oksygenverdier i bunnvann trakk ned. De to ålegress-stasjonene var lokalisert i samme vannforekomst og fikk begge “god” tilstand. I tillegg til parameterne brukt til klassifisering ble klimaparameterne dyreplankton, lys og organisk materiale overvåket ved én stasjon i vannforekomst Steinsfjorden nær Ulsteinvik.MiljødirektoratetpublishedVersio

Simulating MADMAX in 3D: Requirements for dielectric axion haloscopes

We present 3D calculations for dielectric haloscopes such as the currently envisioned MADMAX experiment. For ideal systems with perfectly flat, parallel and isotropic dielectric disks of finite diameter, we find that a geometrical form factor reduces the emitted power by up to 30 % compared to earlier 1D calculations. We derive the emitted beam shape, which is important for antenna design. We show that realistic dark matter axion velocities of 10-3 c and inhomogeneities of the external magnetic field at the scale of 10 % have negligible impact on the sensitivity of MADMAX. We investigate design requirements for which the emitted power changes by less than 20 % for a benchmark boost factor with a bandwidth of 50 MHz at 22 GHz, corresponding to an axion mass of 90 µ eV. We find that the maximum allowed disk tilt is 100 µ m divided by the disk diameter, the required disk planarity is 20 µ m (min-to-max) or better, and the maximum allowed surface roughness is 100 µ m (min-to-max). We show how using tiled dielectric disks glued together from multiple smaller patches can affect the beam shape and antenna coupling. © 2021 The Author(s)