Investigation of NF-B1 and NF-BIA Gene Polymorphism in Non-Small Cell Lung Cancer

Lung cancer is a complex, multifactorial disease which is the leading cause of cancer death in both men and women. NF-B is a transcription factor which is known to affect the expression of more than 150 genes related to inflammation, lymphocyte activation, cell proliferation, differentiation, and apoptosis, as well as contributing to cell apoptosis and survival. However, NF-BIA (I B ) is the inhibitor of the transcription factor. The -94ins/delATTG polymorphism of the NF-B1 gene promoter region which causes a functional effect and NF-BIA 3 UTR A → G polymorphism has been shown to be related to various inflammatory diseases and cancer. Ninety-five NSCLC patients and 99 healthy controls were included in study. The NF-B1 -94ins/delATTG and NF-BIA 3 UTR A → G polymorphism have been studied by using PCR-RFLP method. It was found that the NF-B1 -94ins/delATTG DD genotype and D allele frequencies were higher in patients than healthy controls and the presence of the DD genotype has a 3.5-fold increased risk of the disease (P: 0.014). This study is the first to investigate the NF-B1 -94ins/delATTG and NF-BIA 3 UTR A → G polymorphism together in the Turkish population. According to the results, the NF-B1 -94ins/del ATTG promoter polymorphism may have a role in lung carcinogenesis and prognosis

Synthesis and A Suggestion Mechanism on Biological Evaluation of Amino Acid-Schiff Base Ligands and Co(II), Cu(II) and Ni(II) Complexes

This study aimed to investigate of the antimicrobial activities of some amino acid-Schiff bases complexes as theoretical aspects. Co(II), Cu(II) and Ni(II) complexes of N, N'-(1,4-phenylenedimetiliden) bis DL-Alanine and N, N'-(1,4-phenylendimetiliden) bis DL-Glisine were been prepared and characterized. The antibacterial and antifungal activities were measured by Disc diffusion and MIC method against gram-positive bacteria i. e. Psydomamonas aeruginosa ATCC 29212, Bacillus subtilis RSKK 244, Bacillus megaterium(clinical isolate), gram-negative bacteria Micrococcus Luteus NRRLB and as fungus Candida albicans. The antibiogram tests of amino acid-Schiff bases complexes showed better results than some known antibiotics. Especially Cu(II) complexes were more potent bacteridal than all of the substances synthesized. Furthermore a mechanism of reaction was offered in the explanation of these observation. Some of the compounds exhibited activity comparable to Ketoconazole, Ampicillin, Tetracycline, Penicillin, Gentamisin and Chloroamphenicol.Gazi University Scientific Research FundGazi University [05/2007-02, 05/2010-03]The authors thank to the Gazi University Scientific Research Fund (Project number: 05/2007-02 and 05/2010-03) for the financial support provided for this study and Ozge Cicek, Esra Yildirim, Yasemin Sahin and Refiye Tekiner for carryings out laboratory studies

Some novel antimicrobial therapeutic agents for acetylcholinesterase inhibitors; synthesis of hydroxyquinoline ester involving amino acid

The aim of this work was to investigate the new effective agents candidate for treatment of the Alzheimer’s disease. So, a series of new and highly active acetylcholinesterase inhibitors derived from hydroxyquinoline ester containing amino acid were synthesized. Antibacterial activities of the molecules were studied by the well-diffusion method against Listeria monocytogenes 4b, Staphylococcus aureus, Escherichia coli, Salmonella typhi H, Brucella abortus, Staphylococcus epidermis sp., Micrococcus luteus, Shigella dysenteria type 10, Bacillus cereus, Pseudomonas putida and antifungal activity against Candida albicans. All the synthesized compounds behave as inhibitors against acetylcholinesterase enzyme. CysEs and MetEs show more inhibition potency. © 2015, Gazi University Eti Mahallesi. All rights reserved

Long-term efficacy of different doses of steroid in hospitalized COVID-19 patients: A prospective cohort study

Introduction The aim of our study was to investigate the long-term effects of different doses of steroid therapy given in the acute period in hospitalized coronavirus disease 2019 (COVID-19) patients. Methods A total of 78 patients who were followed up in the hospital due to COVID-19 pneumonia and were discharged from the hospital more than 3 months ago, were included in our study. The patients were divided into 3 groups according to the steroid doses they received. Results The value of peak expiratory flow (PEF) was significantly lower in pulse-dose steroid group compared to high-dose steroid group (p=0.040). There was a significant difference in the values of carbon monoxide diffusion capacity (DLCO) and diffusion coefficient of alveolar volume (DLCO/VA) among all groups (p=0.027, p=0.048). The values of DLCO and DLCO/VA were significantly lower in the pulse-dose steroid group compared to the high-dose steroid group (p=0.022, p=0.049). The computed tomography (CT) score was significantly higher in the pulse-dose steroid group compared to both the nonsteroid group and high-dose steroid group (p=0.027, p=0.043). Conclusions Pulse-dose steroid therapy given in the acute period of COVID-19 pneumonia may negatively affect pulmonary function and recovery in the long-term. In addition, high-dose steroids were found to have a positive effect on pulmonary function compared to pulse-steroid therapy and nonsteroid therapy

Demographic and clinical characteristics and bone-mineral metabolism of pediatric cerebral palsy patients [Serebral palsili hastalarin klinik bulgulari ve kemik mineral metabolizmalarinin degerlendirilmesi]

The aim of the study is to evaluate clinic and demographic properties, detect bone mineral metabolism problems and raise awareness of these problems of cerebral palsy (CP) patients who are followed up in Çukurova University Pediatric Neurology Department. 102 patients aged 2-18 years who are regularly followed up in Çukurova University Pediatric Neurology Department, between January 2014-January 2015 are involved in this study. Demographic characteristics, etiologies, clinic and laboratory findings and associated problems of these patients are evaluated. Mean age of patients were 96.83 ± 53.54 months (24-207 months), male/female ratio was 1,2. 23.5% of the percents were born term and 50% patients' birth weight was above 2500 gr. Spastic Quadriplegia was the most frequent type (38.2%). Most frequent etiologic factor was natal factors (56.9%). In detailed etiologic evaluation, asphyxia (49%) and low birth weight (28.4%) were most seen. Most frequent cerebral MRI abnormality was periventricular leukomalacia (25%). Other accompanying problems were mental retardation 88.2%, speech problems (72.5%), malnutrition (67.6%), epilepsy (46.4%), visual problems (28.4%). Levels of osteocalcin, IGF-1, IGFBP-3 and PTH were better in patients who were on regular physiotherapy (p 0.05). Enough and correct sun exposure and regular, dynamic physiotherapy may improve bone health. Regular follow-up of neuromotor development in patients with complicated birth and perinatal asphyxia may prevent delayed diagnosis and treatment