RAPD markers associated with resistance to blackleg disease in Brassica species

Blackleg, caused by Leptosphaeria maculans, is a serious disease of Brassica species. Genetic analysis of resistance to L. maculans was carried out with 15 accessions from the USDA Brassica germplasm collections, representing diploids (A, C), and tetraploid (AC) genomes, respectively; and 9 cultivars from the National Winter Canola Variety Trials (NWCVT) all carrying AC genomes. All genotypes were screened for blackleg disease at the cotyledonary stage. The results indicated that 46% of the 24 genotypes were resistant, while 54% were susceptible. On the other hand, adult plant screening revealed that all the public genotypes were resistant. In an effort to identify molecularmarkers associated with resistance to blackleg disease, all genotypes were screened with 13 RAPD and 8 SSR markers producing 169 amplified products. Six RAPD markers (OPB01, OPE03, OPE16, OPF10, OPE12, and OPI01) were polymorphic, while the SSR markers were monomorphic. Chi-square analysis indicated that 5 amplified fragments (OPE03-4000, OPE16-1100, OPE16-1300, OPE16-1900, and OPI01- 280) from RAPD primers were significantly associated with blackleg resistance. Thus this study demonstrated that RAPD primers could be effectively used to identify DNA markers that are associatedwith blackleg disease resistance, and that resistance to L. maculans might also exist in the A and C genomes

Phylogenetic relationships within and among Brassica species from RAPD loci associated with blackleg resistance

The genus Brassica comprises economically important oilseed and vegetable crops. Their susceptibility to fungal diseases such as blackleg causes yield loss. In this study, thirty accessionsfrom USDA germplasm collection representing two diploid Brassica species (Brassica rapa and Brassica oleracea var. virids) and fifteen tetraploid cultivars (Brassica napus) from the national wintercanola variety trials (NWCVT) were evaluated using 13 sets of random amplified polymorphic DNA (RAPD) associated with blackleg resistance in Brassica nigra. 126 highly polymorphic bands with an average of 10 per primer were detected. A UPGMA dendrogram showed B. rapa as highly diverse and was supported from three different basal branches, while B. napus accessions were generally monophyletic. Similarly, all of B. oleraceae accessions were supported from the same basal node.Generally, the three species were reciprocally paraphyletic, suggesting that the RAPD markers showed both functional relationships as well as homology, possibly due to selection at the RAPD loci associated with blackleg resistance. Consequently, two potentially susceptible B. napus accessions were identified. The high polymorphic information content (PIC) and number of phylogenetically informative bands established RAPD as a useful tool for phylogenetic reconstruction, quantification ofgenetic diversity for conservation, cultivar classification and molecular breeding in Brassica

COVID-19 pandemic:Nigerian University lecturers’ response to virtual orientation

The effect of the COVID-19 pandemic globally and the disruptions occasioned by it has far-reaching implications on societies in general and most especially the educational sector with governments across the globe ordering lockdowns including the closure of education institutions thereby necessitating alternative teaching and learning methods other than the usual face-to-face interaction to avoid a total collapse of the education sector. This study investigates Nigerian University lecturers’ perspective and response to virtual learning as an alternative to face-to-face teaching method during the pandemic. Relying on primary data source collected using questionnaires, a total number of 435 lecturers responded across both public and private universities and their responses analysed using SPSS. The study found that lecturers from private universities responded to virtual teaching than those from public universities; that the presence of infrastructural orientation influences virtual orientation; and that a negative relationship exists between the sociodemographic/occupational variables (gender, current position, years of experience) and virtual orientation of lecturers of Nigerian universities

Anthocyanin management in fruits by fertilization

Anthocyanins are water-soluble vacuolar plant pigments that are mainly synthesized in epidermal layers and the flesh of fruits such as apples, cherries, grapes, and other berries. Because of their attractive red to purple coloration and their health-promoting potential, anthocyanins are significant determinants for the quality and market value of fruits and fruit-derived products. In crops, anthocyanin accumulation in leaves can be caused by nutrient deficiency which is usually ascribed to insufficient nitrogen or phosphorus fertilization. However, it is a little-known fact that the plant’s nutrient status also impacts anthocyanin synthesis in fruits. Hence, strategic nutrient supply can be a powerful tool to modify the anthocyanin content and consequently the quality and market value of important agricultural commodities. Here we summarize the current knowledge of the influence of plant nutrients on anthocyanin synthesis in fruits of major global market value and discuss the underlying cellular processes that integrate nutrient signaling with fruit anthocyanin formation. It is highlighted that fertilization that is finely tuned in amount and timing has the potential to positively influence the fruit quality by regulating anthocyanin levels. We outline new approaches to enrich plant based foods with health-promoting anthocyanins

Regional situation analysis on out-of-school children in the Eastern and Southern Africa region

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Being a young Muslim woman in Southern Ghana: intersections of nation, religion and gender

This paper draws upon recent research that explored Muslim youth identity constructions in southern Ghana. At a time when Muslim youth are often demonised and Islam is represented as incompatible with western democratic ideals, the research explored the intersections of nation, religion, ethnicity and gender in identity narratives of female students in high school and higher education in southern Ghana. After describing the Ghanaian socio-historical context, we outline the post-structural and post-colonial theories used to understand key concepts such as identity and youth. We then describe our methodological approach before presenting our intersectional analysis of data from the focus group discussions. This highlights female youths’ deep commitments to nation and religion, the marginalisation of young Muslim females in this Christian-majority context, and finally, the identity tensions articulated by participants between different Islamic traditions. We further show how all of these identity constructions were intersected by gender

Examining the Effect of Community Participation on Beneficiary Satisfaction with the work of Water Management Committee in Urban Community-based Operated Water Schemes

Community participation (CP) has become popular in development discourse and practice, particularly in relation to water resource management. Greater involvement of beneficiaries in decision-making and implementation of water management practices is expected to increase efficiency and equity. However, the lingering question remains—Does CP necessarily result in outcomes of greater beneficiary satisfaction? Relatively little is known about the linkage. This article seeks to examine the relationship between community participation and beneficiary satisfaction with the work of the water management committee. Four urban-based community operated water schemes in the city of Kisumu, Kenya, are used as empirical referent. We applied quantitative research methodology by conducting a detailed survey and logistic regression analytic technique to analyze the data. We used seven parameters/indicators to operationalize participation and one to ascertain satisfaction with the work of the water management committee. Our results indicate that five participatory variables correlate with beneficiary satisfaction with the work of the water management committees including provision of labor (p \u3c .05), willingness to intervene against vandalism (p \u3c .05), meeting attendance (p \u3c .05), financial contribution (p \u3c .05) and payments of water bills on time (p \u3c .05). These findings suggest that managers of community water supplies projects together with development partners need to encourage the identified participatory variables as a means of augmenting beneficiary satisfaction and most importantly improving effectiveness/sustainability

Electricity Supply, and Access to Water and Improved Sanitation as Determinants of Gender-Based Inequality in Educational Attainment in Africa

The central hypothesis of this study is that gender-based inequality in education in Africa depends to a significant degree on electricity supply, and access to water and improved sanitation. Gender-based educational inequality is operationalized in terms of the proportion of females to males within any given group of educated people. Three groups, people with basic literacy skills, people with primary education, and people with a secondary education, are considered. Logarithmically-transformed multiple regression analyses, with R2 values ranging from .26 to .55, confirmed the central hypothesis in all but one instance. The instance concerns the hypothesized positive link between access to improved sanitation and females with a secondary education. An analysis of the data revealed this relationship as negative. This paradoxical revelation is explained as follows. Recent positive trends in African economies have occasioned improvements in sanitation that are unmatched by a corresponding increase in female secondary education. The confirmed positive link between access to basic services and female education is easy to explain. The availability of basic services facilitates execution of domestic chores hence, free up time for girls and women to pursue educational opportunities. The article’s significance resides in its empirical validation of the following widely-held but hardly interrogated view. The progress of women in Africa is significantly retarded by the fact that they are overburdened by domestic chores