4,408 research outputs found
De novo Development and Characterization of Tetranucleotide Microsatellite Loci Markers from a Southeastern Population of the House Finch (Haemorhous mexicanus)
Microsatellites are short tandem repeats (e.g. TAGATAGA) of base pairs in a species’ genome. High mutation rates in these regions produce variation in the number of repeats across individuals that can be utilized to study patterns of population- and landscape-level genetics and to determine parentage genetically. In this project our objective was to develop microsatellite markers for the House Finch, Haemorhous mexicanus. This species has become one of the most well-studied species of songbirds due to its unique geographical, evolutionary, and epidemiological history. Using mist-nets we captured birds on the Arkansas Tech University campus and collected blood samples to obtain genomic DNA. Samples were processed in The Field Museum’s Pritzker Laboratory for Molecular Systematics and Evolution, where we fragmented genomic DNA and isolated fragments that contained potential microsatellites using specially designed biotin labelled probes. These DNA fragments were transformed into competent E. coli cells which were then PCR-amplified and Sanger sequenced. After sequencing DNA fragments from approximately 500 E. coli colonies, we designed and characterized a set of 13 tetranucleotide microsatellite loci. The average number of alleles and heterozygosity found in 12 individuals from Arkansas was 8.69 and 0.80, respectively. This finalized set of microsatellites can be utilized by researchers to determine parentage and characterize genetic differences across House Finch populations
Perceptual-gestural (mis)mapping in serial short-term memory: The impact of talker variability
The mechanisms underlying the poorer serial recall of talker-variable lists (e.g., alternating female–male voices) as compared with single-voice lists were examined. We tested the novel hypothesis that this talker variability effect arises from the tendency for perceptual organization to partition the list into streams based on voice such that the representation of order maps poorly onto the formation of a gestural sequence-output plan assembled in support of the reproduction of the true temporal order of the items. In line with the hypothesis, (a) the presence of a spoken lead-in designed to further promote by-voice perceptual partitioning accentuates the effect (Experiments 1 and 2); (b) the impairment is larger the greater the acoustic coherence is between nonadjacent items: Alternating-voice lists are more poorly recalled than four-voice lists (Experiment 3); and (c) talker variability combines nonadditively with phonological similarity, consistent with the view that both variables disrupt sequence output planning (Experiment 4). The results support the view that serial short-term memory performance reflects the action of sequencing processes embodied within general-purpose perceptual input-processing and gestural output-planning systems
Retrieval from memory: Vulnerable or inviolable?
We show that retrieval from semantic memory is vulnerable even to the mere presence of speech. Irrelevant speech impairs semantic fluency—namely, lexical retrieval cued by a semantic category name—but only if it is meaningful (forward speech compared to reversed speech or words compared to nonwords). Moreover, speech related semantically to the retrieval category is more disruptive than unrelated speech. That phonemic fluency—in which participants are cued with the first letter of words they are to report—was not disrupted by the mere presence of meaningful speech, only by speech in a related phonemic category, suggests that distraction is not mediated by executive processing load. The pattern of sensitivity to different properties of sound as a function of the type of retrieval cue is in line with an interference-by-process approach to auditory distraction
On The Origins of the Cute as a Dominant Aesthetic Category in Digital Culture
In discussions of online culture, nobody has yet given sufficient consideration to the importance of cute animal pictures. While there are perhaps obvious reasons for this aspect of online culture being and remaining understudied, from an objective stance we should consider it both surprising and noteworthy that, once given the means of mass communications and internationally accessible publication, a primary activity that people are interested in and committed to is the sharing of cute and funny pictures, especially of cats. This presumably unforeseeable outcome is made stranger yet by the relative lack of commercial motivation for a communications category that approaches the ubiquity of spam and pornography. This chapter investigates three possible explanations of aspects of these phenomena
The Original Progressive Farmer: The Agricultural Legacy of Thomas Spalding of Sapelo
During the first half of the nineteenth century, a thriving plantation emerged on Sapelo Island, Georgia. The plantation’s owner was Thomas Spalding (1774-1851), who was one of Georgia’s foremost planters, and yet his substantial contributions to Georgia’s agricultural development have gone unnoticed by most historians. Spalding built a prosperous enterprise around staple crops such as Sea Island cotton; however, he was better known for his experiments with novel crops such as sugar cane, as well as his innovations in the areas of crop rotation and diversification and the successful implementation of tabby as a viable construction material on the Georgia coast. This paper analyzes these areas of Spalding’s career with particular attention given to his extensive writings on the subjects in the leading agricultural journals of the period. Spalding’s accomplishments are many, and this study seeks to shed new light on his most noteworthy agricultural endeavors and to demonstrate that his life and career deserve further investigation by historians
Validation of Subject Specific Computed Tomography-based Finite Element Models of the Human Proximal Tibia using Full-field Experimental Displacement Measurements from Digital Volume Correlation
Quantitative computed tomography-based finite element (QCT-FE) modeling is a computational tool for predicting bone’s response to applied load, and is used by musculoskeletal researchers to better understand bone mechanics and their role in joint health. Decisions made at the modeling stage, such as the method for assigning material properties, can dictate model accuracy. Predictions of surface strains/stiffness from QCT-FE models of the proximal tibia have been validated against experiment, yet it is unclear whether these models accurately predict internal bone mechanics (displacement). Digital volume correlation (DVC) can measure internal bone displacements and has been used to validate FE models of bone; though, its use has been limited to small specimens.
The objectives of this study were to 1) establish a methodology for high-resolution peripheral QCT (HR-pQCT) scan acquisition and image processing resulting in low DVC displacement measurement error in long human bones, and 2) apply different density-modulus relationships and material models from the literature to QCT-FE models of the proximal tibia and identify those approaches which best predicted experimentally measured internal bone displacements and related external reaction forces, with highest explained variance and least error.
Using a modified protocol for HR-pQCT, DVC displacement errors for large scan volumes were less than 19ÎĽm (0.5 voxels). Specific trabecular and cortical models from the literature were identified which resulted in the most accurate QCT-FE predictions of internal displacements (RMSE%=3.9%, R2>0.98) and reaction forces (RMSE%=12.2%, R2=0.78). This study is the first study to quantify experimental displacements inside a long human bone using DVC. It is also the first study to assess the accuracy of QCT-FE predicted internal displacements in the tibia. Our results indicate that QCT-FE models of the tibia offer reasonably accurate predictions of internal bone displacements and reaction forces for use in studying bone mechanics and joint health
Social Media and the Organization Man
On new dynamics in organizational psychology, self- and group-identity, character, and integrity in an age of social media, Organizations may then have a similar relation to our integrity as does our character. Our character is formed by a history of actions and interactions, but we may not identify with the actions that it brings us to habitually perform. When we recognize our vices—e.g., intemperance—and seek to act in accordance with our values and beliefs, we act against our character and contribute thereby to reforming our habits and character to better align with the version of ourselves with which we identify. Organizations may similarly bring us, through their own form of inertia and habituation, to act in ways contrary to our values and beliefs. A confrontation with this contradiction through context collapse may help us to better recognize the organization’s vices and to act according to the version of ourselves, in that organizational context, with which we identify—and contribute thereby to reforming our organization to better align with our values, and with its values as well
Against Strong Copyright in E-Business
As digital media give increasing power to users—power to reproduce, share, remix, and otherwise make use of content—businesses based on content provision are forced to either turn to technological and legal means of disempowering users, or to change their business models. By looking at Lockean and Kantian theories as applied to intellectual property rights, we see that business is not justified in disempowering users in this way, and that these theories obligate e-business to find new business models. Utilitarian considerations support disempowering users in this way in some circumstances and for the time being, but also show that there is a general obligation to move to new business models. On these moral bases, as well as on practical bases, e-business ought to refrain from using the legally permitted strong copyright protections, and should instead find ways of doing business which support, value, and respect the technical capabilities that users have gained
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