Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

The present study established a protocol for suspension cell culture of Stevia rebaudiana and demonstrates the antioxidant enzymes activity during various developing phase of it. Higher concentration of Auxin and Cytokines (3.0 mg L-1 BAP and 4.0 mg L-1 NAA) with Ascorbic acid (1 mg L-1) considered as highly suitable growth regulator combination for growth and development of Stevia rebaudiana suspension culture. Maximum concentration of stevioside (~70 mg G-1 of dry weight of tissue) was obtained on 14th day (exponential phase) which got reduced on 28th day (apoptotic phase) upto ~21 mg G-1 of dry weight of tissue. The amount of MDA reduced initially upto stationary growth phase which showed the adaptation of suspension cells in the culture medium and culture environment. Reduction of Chlorophyll showed the enhancing phenolic content with progressive culture period, while enhanced amount of proline was indicating the generation of defense mechanism with depletion of nutrient (with increased culture duration). The amount of SOD got enhanced with elevating the concentration of H2O2 as well. The concentration of CAT enhanced upto stationary growth phase of suspension cell while APX concentration showed continuous inhibition up to apoptotic phase from exponential phase

QSAR STUDIES, AND IN SILICO ADME PREDICTION OF P-AMINOSALICYLIC ACID DERIVATIVES AS NEURAMINIDASE INHIBITORS

QSAR analysis on a set of  synthesized p-Aminosalysilic Acid derivatives analogues tested growth inhibitory antiviral activity was performed by using MLR procedure. The activity contribution of these compounds were determined from regression equation and the validation procedures to analyze the predictive ability of QSAR models were described.The results are discussed on the basis of statistical data. High agreement between experimental  and predicted antiviral activity inhibitory values are obtained. The results revealed the significant roles of topological, geometrical and substituent electronic descriptor parameters on the inhibitory activitypIc50 of coumarin derivative analogues of the studied moleculesKey Words: QSAR, Antiviral Activity, ML

Prophylactic potential of liposomized integral membrane protein of Plasmodium yoelii nigeriensis against blood stage infection in BALB/c mice

Triton X-114 phase separated integral membrane proteins (IMPs) of a multidrug resistant strain of Plasmodium yoelii nigeriensis (P. yoelii) were screened for their potential to impart protection against malaria infection in BALB/c mice. As revealed by immunoblotting, antibodies present in parasite specific sera from convalescent (protected) as well as immunized (partially protected) animals recognized different membrane proteins. A thorough investigation reveals that P. yoelii specific convalescent sera recognized IMPs with molecular masses ranging from 21 to 81 kDa. Among various membrane proteins, the IMPs corresponding to 81 and 66 kDa molecular weight were highly prominent in the immunoblots probed with the sera from convalescent animals, whereas sera from immunized animals failed to produce impressive band pattern. Immunofluorescence assay revealed that the 66-kDa IMP specific antibodies reacted with fixed smears of mature schizonts and merozoites. Further immunization with 66 kDa IMP (PyIMP) purified through polyclonal IgG sepharose 4B affinity did not impart effective immune response (in its free form) and could provided partial protection only. On the other hand, animals immunized with 66 kDa PyIMP entrapped in phosphatidyl-choline/cholesterol (PC/chol) liposomes protected BALB/c mice against lethal P. yoelii challenge

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

The present day modern formulation practices for drugs are based on newer tools and techniques toward effective utilization. The methods of antibody formulations are to be revolutionized based on techniques of cell engineering and gene editing. In the present review, we have discussed innovations in cell engineering toward production of novel antibodies for therapeutic applications. Moreover, this review deciphers the use of RNAi, ribozyme engineering, CRISPR-Cas-based techniques for better strategies for antibody production. Overall, this review describes the multidisciplinary aspects of the production of therapeutic proteins that has gained more attention due to its increasing demand

A Comprehensive Review on Plant Disease Vectors and Their Management

Plant disease vectors play a critical role in agricultural productivity and ecosystem health. This comprehensive review explores the intricate nature of these vectors and their role in transmitting major plant diseases, including those of viral, bacterial, and fungal origins. It delves into the mechanisms underlying disease transmission, and the significant factors that influence vector efficiency. The socioeconomic and ecological impacts of these vectors are highlighted, with emphasis on crop yield reductions and ecological imbalances. Traditional and emerging vector management methods, such as chemical control, biological control, cultural practices, genetic engineering, and precision agriculture are examined. The review also addresses the challenges inherent to vector management, including resistance development, non-target effects, environmental factors, and socioeconomic barriers. Future perspectives are offered, emphasizing the need for sustainable strategies, exploitation of emerging technologies, enhanced surveillance, community involvement, policy support, and preparation for forthcoming challenges due to climate change, land-use alterations, and global trade. This extensive review presents a critical resource for stakeholders in plant disease vector management, guiding future research directions and policy-making